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[186927_s_at]

Description Y65B4BR.6 /REP_DB=WormBase Gene ID /WP=CE25541 /TR=Q9N2Z3 /GB=AAF60859.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 43      4grp_highest_8_8 > 02      4grp_high_8_8 > 54      6grp_highest_nonorm > 33      6grp_total > 0      4grp_total > 49      6grp_total_sub > Hat      6grp_highest_sub > 02      6grp_cgc > 54      6grp_high1_sub > 02      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4924
1 2 3 4 range
A 173.5
(-3)
P 		4154.2
(2.2)
P 		4807.3
(3)
P 		193.9
(-3)
P 		4634
B 191.8
(-3)
P 		4384.5
(2.1)
P 		5095.3
(3)
P 		198.3
(-3)
P 		4904
C 171.3
(-3)
P 		4247.4
(3)
P 		4275
(3)
P 		220.3
(-2.9)
P 		4104
avr 178.9
(-3) 		4262
(2.4) 		4725.9
(3) 		204.2
(-3) 		4547

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3141
1 2 3 4 5 6 range
A 426.4
(-3)
P 		3384.1
(3)
P 		1546.1
(-0.7)
P 		1472.6
(-0.9)
P 		1405
(-1)
P 		1649.1
(-0.5)
P 		2958
B 243.4
(-3)
P 		3354.4
(3)
P 		1912.6
(0.2)
P 		1222.5
(-1.1)
P 		1228.1
(-1.1)
P 		1051.1
(-1.4)
P 		3111
C 283.2
(-3)
P 		3134.5
(3)
P 		1966.6
(0.5)
P 		994
(-1.5)
P 		1826.8
(0.2)
P 		969
(-1.6)
P 		2851
avr 317.7
(-3) 		3291
(3) 		1808.4
(0) 		1229.7
(-1.2) 		1486.6
(-0.6) 		1223.1
(-1.2) 		2973
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1462
1 2 3 4 range
A 63.2
(-2.8)
P 		901.8
(1.3)
P 		1256.7
(3)
P 		28.4
(-3)
P 		1228
B 61.2
(-2.9)
P 		1082.9
(2.5)
P 		1180.7
(3)
P 		42.3
(-3)
P 		1138
C 56.7
(-2.9)
P 		1221.2
(1.9)
P 		1490
(3)
P 		37.2
(-3)
P 		1453
avr 60.4
(-2.9) 		1068.6
(1.9) 		1309.1
(3) 		36
(-3) 		1273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1125
1 2 3 4 5 6 range
A 78.6
(-3)
P 		1203.6
(3)
P 		336.7
(-1.6)
P 		373.5
(-1.4)
P 		424
(-1.2)
P 		401.5
(-1.3)
P 		1125
B 84.7
(-3)
P 		1116.5
(3)
P 		464.4
(-0.8)
P 		323.8
(-1.6)
P 		499
(-0.6)
P 		392
(-1.2)
P 		1032
C 105
(-3)
P 		956.4
(3)
P 		500.5
(-0.2)
P 		199.9
(-2.3)
P 		591.1
(0.4)
P 		324.4
(-1.5)
P 		851
avr 89.4
(-3) 		1092.2
(3) 		433.9
(-0.9) 		299.1
(-1.7) 		504.7
(-0.5) 		372.6
(-1.3) 		1003
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186927_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2574
Sequence Type Exemplar sequence
Representative Public ID Y65B4BR.6 NCBI
Target Description Y65B4BR.6 /REP_DB=WormBase Gene ID /WP=CE25541 /TR=Q9N2Z3 /GB=AAF60859.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058353(11), NM_182075(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058353 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-16) (grl-16) mRNA, complete cds. 11/11 None
NM_182075 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-16) (grl-16) mRNA, complete cds. 11/11 None
SNAP00000036983 ENSEMBL cdna:SNAP chromosome:CEL140:I:572440:575069:-1 11/11 None
GENEFINDER00000036990 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:572440:575069:-1 11/11 None
Y65B4BR.6a ENSEMBL cdna:known chromosome:CEL140:I:572180:575122:-1 gene:Y65B4BR.6 11/11 None
Y65B4BR.6b ENSEMBL cdna:known chromosome:CEL140:I:572440:575069:-1 gene:Y65B4BR.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:572439-575069(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y65B4BR.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60859 Ground-like (grd related) protein 16, isoform a [Caenorhabditis elegans] ref|NP_490754.1| GRound-Like (grd related) family member (grl-16) [Caenorhabditis elegans] 1.0E-114
blast CAE61293 Hypothetical protein CBG05117 [Caenorhabditis briggsae] 1.0E-81
blast AAO21416 Ground-like (grd related) protein 16, isoform b [Caenorhabditis elegans] ref|NP_871875.1| GRound-Like (grd related) family member (grl-16) [Caenorhabditis elegans] 4.0E-70
blast AAO21416 Ground-like (grd related) protein 16, isoform b [Caenorhabditis elegans] ref|NP_871875.1| GRound-Like (grd related) family member (grl-16) [Caenorhabditis elegans] 5.0E-67
blast AAF60859 Ground-like (grd related) protein 16, isoform a [Caenorhabditis elegans] ref|NP_490754.1| GRound-Like (grd related) family member (grl-16) [Caenorhabditis elegans] 5.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 4.7E-21
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 4.7E-21
Sequence
Target Sequence 
>C. ELEGANS:186927_S_AT
ggataccaatcgggaccagtcagctctatccaggtgacccaagtctctcagggaggaaac
taccaagctccaccagagcaccaggctgttcaagaaatcgtcgctgagactgaagcacca
gtcgtcgttgcagagaccgaagccccagccgtcgttgagaccgaggctccagttgtgatc
gaggagaccgaggcgccagttgtcgttgaagagactccagctccagttgtcgaggagact
caggctccagttgccgctgccaaggaggagagcacctatgatgatattgttgaggagcag
ccatccaccgccaccgagaccgccaccgagcaagttgatactgttgaggagaccaacgaa
gctgtcgagtacgccgatgaggaaggagacgagggaaactgcgacgatccagagctccgc
gccatcgttgaggccgctttgaagaacgagaaggacaacctcgaggccgccagaaagatc
gagggagacgcctcggccaagttcggaggacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATACCAATCGGGACCAGTCAGCT 684 507 574 Antisense
CAGGTGACCCAAGTCTCTCAGGGAG 267 207 604 Antisense
GAAACTACCAAGCTCCACCAGAGCA 23 357 629 Antisense
CTGAAGCACCAGTCGTCGTTGCAGA 174 237 683 Antisense
CCCAGCCGTCGTTGAGACCGAGGCT 99 259 717 Antisense
GACCGAGGCTCCAGTTGTGATCGAG 509 381 732 Antisense
CCGAGGCGCCAGTTGTCGTTGAAGA 34 265 761 Antisense
CTCCAGTTGCCGCTGCCAAGGAGGA 603 233 818 Antisense
ACCGCCACCGAGCAAGTTGATACTG 700 89 892 Antisense
GGGAAACTGCGACGATCCAGAGCTC 333 493 966 Antisense
ACGCCTCGGCCAAGTTCGGAGGACG 218 89 1061 Antisense
Sequence Source Affymetrix Proprietary Database