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[186924_at]

Description F37C12.12 /REP_DB=WormBase Gene ID /WP=CE00825 /TR=Q20127 /GB=AAC48300.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 37      4grp_total > 02      6grp_cgc > 15      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 74.4
(0.6)
P 		74.2
(0.5)
A 		53.6
(-3)
A 		88.6
(3)
A 		35
B 47.7
(-3)
A 		69.7
(0.2)
A 		86
(2.6)
A 		88.9
(3)
P 		41
C 74.2
(-0.7)
A 		40.5
(-3)
A 		52.8
(-2.2)
A 		128.8
(3)
P 		88
avr 65.4
(-2.4) 		61.5
(-3) 		64.1
(-2.6) 		102.1
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 132.9
(3)
A 		52.9
(-3)
A 		60
(-2.5)
A 		82.2
(-0.8)
A 		56.2
(-2.8)
A 		94.8
(0.1)
A 		80
B 82.4
(3)
M 		57.8
(0.2)
A 		47.7
(-1)
A 		29.8
(-3)
A 		71.1
(1.7)
A 		54.4
(-0.2)
A 		53
C 42.7
(-3)
A 		117.9
(3)
A 		70.2
(-0.8)
A 		90.2
(0.8)
A 		51.9
(-2.3)
A 		67.4
(-1)
A 		75
avr 86
(3) 		76.2
(0.8) 		59.3
(-3) 		67.4
(-1.2) 		59.7
(-2.9) 		72.2
(-0.1) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 27.1
(3)
P 		16.1
(-1.7)
A 		14
(-2.6)
A 		13
(-3)
A 		14
B 15.2
(-3)
A 		17.2
(-0.4)
A 		19.9
(3)
A 		18.9
(1.7)
P 		5
C 24.6
(3)
A 		11.6
(-3)
A 		18.4
(0.1)
A 		21.7
(1.7)
P 		13
avr 22.3
(3) 		15
(-3) 		17.4
(-1) 		17.9
(-0.6) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 24.5
(3)
A 		18.8
(0)
A 		13.1
(-3)
A 		20.9
(1.1)
A 		17
(-0.9)
A 		23.1
(2.3)
A 		11
B 28.7
(2.9)
M 		19.2
(0.2)
A 		11.6
(-1.9)
A 		7.9
(-3)
A 		28.9
(3)
A 		20.3
(0.5)
A 		21
C 15.8
(-3)
A 		36
(3)
A 		17.9
(-2.4)
A 		18.1
(-2.3)
A 		16.8
(-2.7)
A 		22.6
(-1)
A 		20
avr 23
(2) 		24.7
(3) 		14.2
(-3) 		15.6
(-2.2) 		20.9
(0.8) 		22
(1.5) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186924_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7791
Sequence Type Exemplar sequence
Representative Public ID F37C12.12 NCBI
Target Description F37C12.12 /REP_DB=WormBase Gene ID /WP=CE00825 /TR=Q20127 /GB=AAC48300.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066179(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066179 NCBI Caenorhabditis elegans MEChanosensory abnormality family member (mec-14) (mec-14) mRNA, complete cds. 11/11 None
SNAP00000030597 ENSEMBL cdna:SNAP chromosome:CEL140:III:7190290:7192868:-1 11/11 None
GENEFINDER00000030607 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7190290:7192868:-1 11/11 None
F37C12.12 ENSEMBL cdna:known chromosome:CEL140:III:7190131:7192916:-1 gene:F37C12.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7190287-7192866(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mec-14
Ensembl F37C12.12
Entrez Gene 185423 Entrez gene
SwissProt Q20127 EMBL-EBI
WormBase CE00825 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:253307_AT L-galactose dehydrogenase (L-GalDH) at
DROSOPHILA_2:1626248_AT dm
DROSGENOME1:149722_AT dm
Protein Similarities
Method ID Description E-Value
blast AAC48300 Mechanosensory abnormality protein 14 [Caenorhabditis elegans] ref|NP_498580.1| MEChanosensory abnormality family member (mec-14) [Caenorhabditis elegans] 0.0
blast CAE70151 Hypothetical protein CBG16615 [Caenorhabditis briggsae] 0.0
blast XP_624237 PREDICTED: similar to GA14985-PA [Apis mellifera] 4.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 1.3E-19
Sequence
Target Sequence 
>C. ELEGANS:186924_AT
gattaggcacattggtctgacgggatatccattgggaaaacttgtgtactcgtttagata
tgtttggaatgcgccaaaattatatttttcagtcaacttgtcgattactcgaaatattgc
agtaatcaattctggagcactatgttggggtttgctcacagaaaagggtccaccgccttg
gcatccagcaagtgatgaaattaaagaagcttgcttagcagccactacttactgttcatc
taagaatataagcatatcaaaactagctctagactatgctctcaatttcccgaacgttat
ttgttgtctggttggaatggatagtgtgcaacaagtattagacaacctggaattatccaa
cttttcacgaataacagatgtagagcagagagttcgagatcggataatgcgaaggtatct
agacagactggaaaatgccggttgggaaggcgtagatgtcgctcaatattggaaaaagtt
gaagaagttgggattaaccgcgttggcaactcatcgtcattcatcagttgaaagtcttgc
aagcacattgaacggattttctttatattcttcaaactcgagctccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTAGGCACATTGGTCTGACGGGA 270 421 711 Antisense
GCACTATGTTGGGGTTTGCTCACAG 228 317 847 Antisense
CACAGAAAAGGGTCCACCGCCTTGG 209 147 867 Antisense
CCGCCTTGGCATCCAGCAAGTGATG 539 261 883 Antisense
GAAGCTTGCTTAGCAGCCACTACTT 107 343 916 Antisense
GCAGCCACTACTTACTGTTCATCTA 272 315 928 Antisense
AACTAGCTCTAGACTATGCTCTCAA 634 137 971 Antisense
TGCTCTCAATTTCCCGAACGTTATT 94 579 987 Antisense
TGGGATTAACCGCGTTGGCAACTCA 388 551 1199 Antisense
TGGCAACTCATCGTCATTCATCAGT 195 547 1214 Antisense
TATATTCTTCAAACTCGAGCTCCGA 487 661 1274 Antisense
Sequence Source Affymetrix Proprietary Database