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[186885_at]

Description T21D12.9B /REP_DB=WormBase Gene ID /WP=CE28416 /TR=O16779 /GB=AAC48095.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 105.8
(3)
A 		94.4
(1.9)
A 		42.5
(-3)
A 		103.8
(2.8)
A 		63
B 163.9
(3)
A 		72.8
(-3)
A 		87.7
(-2)
A 		153.1
(2.3)
A 		91
C 128.3
(2.6)
A 		131.5
(3)
A 		77.9
(-3)
A 		89.4
(-1.7)
A 		54
avr 132.7
(3) 		99.6
(-0.1) 		69.4
(-3) 		115.4
(1.4) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 196.2
(3)
A 		176
(1.4)
M 		172.5
(1.1)
P 		183.8
(2)
P 		121.8
(-3)
A 		181.2
(1.8)
A 		74
B 152.6
(-2.4)
M 		252.7
(3)
P 		150.9
(-2.5)
A 		182.2
(-0.8)
P 		154.8
(-2.3)
A 		141.6
(-3)
A 		111
C 156.3
(-1.3)
M 		196.8
(3)
M 		166.4
(-0.2)
A 		179.4
(1.1)
M 		140.6
(-3)
A 		186.3
(1.9)
P 		56
avr 168.4
(-0.5) 		208.5
(3) 		163.3
(-0.9) 		181.8
(0.7) 		139.1
(-3) 		169.7
(-0.4) 		69
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 38.5
(3)
A 		20.5
(-0.9)
A 		11.1
(-3)
A 		15.2
(-2.1)
A 		27
B 52.3
(3)
A 		18
(-3)
A 		20.3
(-2.6)
A 		32.6
(-0.4)
A 		34
C 42.5
(3)
A 		37.8
(2)
A 		27.1
(-0.4)
A 		15.1
(-3)
A 		27
avr 44.4
(3) 		25.4
(-1.6) 		19.5
(-3) 		21
(-2.6) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 36.2
(-3)
A 		62.6
(3)
M 		37.6
(-2.7)
P 		46.6
(-0.6)
P 		36.7
(-2.9)
A 		44.1
(-1.2)
A 		26
B 53.1
(-0.9)
M 		84.1
(3)
P 		36.6
(-3)
A 		48.3
(-1.5)
P 		62.9
(0.3)
A 		52.8
(-1)
A 		48
C 57.9
(2)
M 		60
(2.5)
M 		42.3
(-1.6)
A 		36.1
(-3)
M 		45.5
(-0.9)
A 		62.4
(3)
P 		26
avr 49.1
(-0.9) 		68.9
(3) 		38.8
(-3) 		43.7
(-2) 		48.4
(-1.1) 		53.1
(-0.1) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186885_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11180
Sequence Type Exemplar sequence
Representative Public ID T21D12.9B NCBI
Target Description T21D12.9B /REP_DB=WormBase Gene ID /WP=CE28416 /TR=O16779 /GB=AAC48095.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067494(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067494 NCBI Caenorhabditis elegans T21D12.9b (T21D12.9) mRNA, complete cds. 11/11 A 
SNAP00000019191 ENSEMBL cdna:SNAP chromosome:CEL140:IV:283938:287029:1 11/11 None
GENEFINDER00000019196 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:283647:287029:1 11/11 None
T21D12.9b ENSEMBL cdna:known chromosome:CEL140:IV:283647:287201:1 gene:T21D12.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176849 3 1 NM_067494 186885_at (11)
NM_067495 174086_s_at (11), 186872_s_at (11)
NM_067496 174086_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:275502-286905(+) 96.95 96.95
Public Domain and Genome References
Ensembl T21D12.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK72064 Hypothetical protein T21D12.9b [Caenorhabditis elegans] ref|NP_499895.2| T21D12.9b [Caenorhabditis elegans] 0.0
blast T28715 hypothetical protein T21D12.9b - Caenorhabditis elegans 0.0
blast CAA97442 Hypothetical protein R13.3 [Caenorhabditis elegans] ref|NP_502007.2| R13.3 [Caenorhabditis elegans] gb|AAR99658.1| bestrophin 1 [Caenorhabditis elegans] sp|Q21973|BES1_CAEEL Bestrophin-1 1.0E-145
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499895.2 5 36-55,70-92,99-116,230-252,272-294
T21D12.9b 5 36-55,70-92,99-116,230-252,272-294
SNAP00000019191 2 148-170,190-212
GENEFINDER00000019196 5 36-55,70-92,99-116,230-252,272-294
Sequence
Target Sequence 
>C. ELEGANS:186885_AT
ggatcagctgccacttatgaaacccaagaaaacgagatcatcatgcgaccccacgttgat
acaaacgacatggaagatggcggtgatgacgtggaaggatgcaatccacgtcaactctca
cgtttcgtgtccgtggtgtctgttaatagaaattgtgagagccggacgagtctatcgagt
cggcggaatccgacgatcatcgaacggttgagaagtcagtttggaggatcgaaagtcaac
aggccgggcaaattattcggcagctcgcagttttccatcaatactgcgatgggagataac
gacattggaagctgtgcaagtatcctgggtgagcttgccgaagagtccaacaaagcatca
caaggtctcttaacacctgaagaatacgcaggctctccacgtcgaaatacccctatcgat
atgctgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCAGCTGCCACTTATGAAACCC 680 509 3466 Antisense
AAAACGAGATCATCATGCGACCCCA 601 131 3494 Antisense
TCCACGTCAACTCTCACGTTTCGTG 283 589 3570 Antisense
TTTCGTGTCCGTGGTGTCTGTTAAT 114 671 3588 Antisense
GAGAGCCGGACGAGTCTATCGAGTC 1 395 3622 Antisense
GGCGGAATCCGACGATCATCGAACG 229 541 3647 Antisense
AATTATTCGGCAGCTCGCAGTTTTC 694 173 3716 Antisense
GAAGCTGTGCAAGTATCCTGGGTGA 273 343 3773 Antisense
GTGAGCTTGCCGAAGAGTCCAACAA 458 483 3794 Antisense
TGAAGAATACGCAGGCTCTCCACGT 366 575 3843 Antisense
GAAATACCCCTATCGATATGCTGAC 129 363 3869 Antisense
Sequence Source Affymetrix Proprietary Database