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[186876_at]

Description C14A4.3 /REP_DB=WormBase Gene ID /WP=CE02144 /TR=SW:P54002 /GB=CAA90107.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 20      6grp_total > 40      4grp_total > 62      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 272
1 2 3 4 range
A 534.8
(3)
P 		380.9
(-3)
A 		492.6
(1.4)
A 		394.2
(-2.5)
A 		154
B 617.1
(3)
P 		530.6
(1.1)
A 		499.7
(0.4)
M 		345
(-3)
M 		272
C 581.3
(2.4)
P 		485.2
(-0.5)
A 		601.2
(3)
A 		401.8
(-3)
P 		199
avr 577.7
(3) 		465.6
(-0.4) 		531.2
(1.6) 		380.3
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 417
1 2 3 4 5 6 range
A 667.1
(0.4)
P 		709.8
(1.3)
P 		788.2
(3)
P 		619.5
(-0.6)
P 		509.1
(-3)
A 		540.7
(-2.3)
P 		279
B 528.9
(-1.2)
P 		742.5
(2.1)
P 		587.3
(-0.3)
M 		803.7
(3)
A 		411
(-3)
P 		643.7
(0.6)
A 		393
C 576.1
(0.7)
M 		586
(0.9)
P 		587.8
(0.9)
P 		695.3
(3)
P 		387.2
(-3)
P 		436.2
(-2)
P 		308
avr 590.7
(0.4) 		679.4
(2.4) 		654.4
(1.9) 		706.2
(3) 		435.8
(-3) 		540.2
(-0.7) 		270
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 194.7
(3)
P 		82.7
(-1.9)
A 		128.8
(0.1)
A 		57.7
(-3)
A 		137
B 196.8
(3)
P 		131
(-0.2)
A 		115.8
(-0.9)
M 		73.5
(-3)
M 		123
C 192.4
(2.3)
P 		139.5
(0)
A 		209.5
(3)
A 		67.8
(-3)
P 		142
avr 194.6
(3) 		117.7
(-0.6) 		151.4
(1) 		66.3
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 123
(-3)
P 		252.4
(3)
P 		171.6
(-0.7)
P 		157.1
(-1.4)
P 		153.6
(-1.6)
A 		131.6
(-2.6)
P 		129
B 184
(-0.6)
P 		247.1
(3)
P 		142.6
(-3)
M 		212.9
(1)
A 		167
(-1.6)
P 		240
(2.6)
A 		105
C 213.5
(3)
M 		178.8
(0.6)
P 		149.6
(-1.3)
P 		139.8
(-2)
P 		125.3
(-3)
P 		146
(-1.6)
P 		88
avr 173.5
(-1.1) 		226.1
(3) 		154.6
(-2.5) 		169.9
(-1.4) 		148.6
(-3) 		172.5
(-1.1) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186876_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2724
Sequence Type Exemplar sequence
Representative Public ID C14A4.3 NCBI
Target Description C14A4.3 /REP_DB=WormBase Gene ID /WP=CE02144 /TR=SW:P54002 /GB=CAA90107.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063881
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
C14A4.3 ENSEMBL cdna:known chromosome:CEL140:II:10584255:10587839:1 gene:C14A4.3 None
SNAP00000002485 ENSEMBL cdna:SNAP chromosome:CEL140:II:10584255:10592469:1 None
GENEFINDER00000002500 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10584255:10587791:1 None
NM_063881 NCBI Caenorhabditis elegans C14A4.3 (C14A4.3) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10584251-10587605(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C14A4.3
Entrez Gene 174633 Entrez gene
SwissProt P54002 EMBL-EBI
WormBase CE32796 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256110_AT curculin-like (mannose-binding) lectin family protein at
CANINE:1599318_AT similar to disrupted in bipolar disorder 1 cfa
CANINE_2:CFA.8267.1.A1_AT similar to disrupted in bipolar disorder 1 cfa
CANINE_2:CFAAFFX.21808.1.S1_S_AT similar to disrupted in bipolar disorder 1 cfa
DROSOPHILA_2:1624152_AT dm
DROSGENOME1:154932_AT dm
HG-U133_PLUS_2:219374_S_AT asparagine-linked glycosylation 9 homolog (yeast, alpha 1,2 mannosyltransferase) hs
HG-U133A:219374_S_AT asparagine-linked glycosylation 9 homolog (yeast, alpha 1,2 mannosyltransferase) hs
HG-U133A_2:219374_S_AT asparagine-linked glycosylation 9 homolog (yeast, alpha 1,2 mannosyltransferase) hs
U133_X3P:G13376061_3P_A_AT asparagine-linked glycosylation 9 homolog (yeast, alpha 1,2 mannosyltransferase) hs
HG-U95E:76266_AT asparagine-linked glycosylation 9 homolog (yeast, alpha 1,2 mannosyltransferase) hs
MG-U74BV2:116678_AT disrupted in bipolar disorder 1 homolog (human) mm
MOE430A:1418844_AT disrupted in bipolar disorder 1 homolog (human) mm
MOUSE430A_2:1418844_AT disrupted in bipolar disorder 1 homolog (human) mm
MOUSE430_2:1418844_AT disrupted in bipolar disorder 1 homolog (human) mm
MOE430B:1430420_AT disrupted in bipolar disorder 1 homolog (human) mm
MOUSE430_2:1430420_AT disrupted in bipolar disorder 1 homolog (human) mm
MU19KSUBA:TC14734_AT Disrupted in bipolar disorder 1 homolog (human) (Dibd1), mRNA mm
RG-U34C:RC_AI236397_AT disrupted in bipolar affective disorder 1 (predicted) rn
RAE230B:1392422_AT disrupted in bipolar affective disorder 1 (predicted) rn
RAT230_2:1392422_AT disrupted in bipolar affective disorder 1 (predicted) rn
YEAST_2:1774151_AT Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Sc
Protein Similarities
Method ID Description E-Value
blast CAA90107 Hypothetical protein C14A4.3 [Caenorhabditis elegans] ref|NP_496282.2| C14A4.3 [Caenorhabditis elegans] sp|P54002|YTH3_CAEEL Putative glycosyl transferase C14A4.3 in chromosome II 0.0
blast CAA90108 Hypothetical protein C14A4.4a [Caenorhabditis elegans] ref|NP_496283.1| Cell-death-Related Nuclease family member (crn-3) [Caenorhabditis elegans] gb|AAP57299.1| cell death-related nuclease 3 [Caenorhabditis elegans] 0.0
blast CAE59646 Hypothetical protein CBG03060 [Caenorhabditis briggsae] 0.0
blast CAE59647 Hypothetical protein CBG03061 [Caenorhabditis briggsae] 1.0E-168
blast XP_798829 PREDICTED: similar to disrupted in bipolar disorder 1 homolog [Strongylocentrotus purpuratus] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR002121 EMBL-EBI HRDC domain 1.5E-27
Pfam IPR005599 EMBL-EBI Plasmid Maintenance Protein 1.0E-126
Pfam IPR002562 EMBL-EBI 3-5 exonuclease 2.0E-75
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496282.2 9 109-126,131-153,165-187,207-229,242-264,305-327,339-361,371-390,403-422
C14A4.3 9 109-126,131-153,165-187,207-229,242-264,305-327,339-361,371-390,403-422
SNAP00000002485 9 109-126,131-153,165-187,207-229,242-264,305-327,339-361,371-390,403-422
GENEFINDER00000002500 9 109-126,131-153,165-187,207-229,242-264,305-327,339-361,371-390,403-422
Sequence
Target Sequence 
>C. ELEGANS:186876_AT
tccatgatccaattcgtgtatgcgttggaaaagaatggcaccgcttcccttcatcgttct
tcattccacaaacagtttctgatgggaaaaaagtggaaatgcgatttattcaaagtgaat
tccgcggacttttacctaaaccattcttaaaatcggacaagttggtggaagttacacgtc
atataccaacagaaatgaataatctgaatcaagaagaaatatcgagatatgtggatttag
attcatgcgattatgtggttgatgttgatatgccacagtcggatagagaacctgatttca
ggaaaatgcgacaaaactacaaaagaaaaacaaaaaaaaaatggaaaagaattgaaaacg
cgattctacaattttggctaaattccctgtttgaggaaatcaaatttttaaattcaaatt
ttcattctttcccattttttttcgtttttgacaattttggattatttcaagcatactcac
actactccttcctgaagataactggaaaccagtggattcgcttccattcatcgatgtctc
caaatcaacaggattccatgggcttcttcgtgcattttatgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCATGATCCAATTCGTGTATGCGT 683 589 1361 Antisense
TGGAAAAGAATGGCACCGCTTCCCT 424 547 1386 Antisense
GATGTTGATATGCCACAGTCGGATA 154 411 1621 Antisense
AAACGCGATTCTACAATTTTGGCTA 279 127 1716 Antisense
ATTTTGGCTAAATTCCCTGTTTGAG 200 13 1731 Antisense
ATTTCAAGCATACTCACACTACTCC 707 15 1824 Antisense
CACACTACTCCTTCCTGAAGATAAC 473 195 1838 Antisense
TGGATTCGCTTCCATTCATCGATGT 254 551 1873 Antisense
TTCATCGATGTCTCCAAATCAACAG 180 689 1887 Antisense
ATCAACAGGATTCCATGGGCTTCTT 310 27 1904 Antisense
GGGCTTCTTCGTGCATTTTATGTTC 580 493 1920 Antisense
Sequence Source Affymetrix Proprietary Database