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[186874_s_at]

Description Y65B4BL.3 /REP_DB=WormBase Gene ID /WP=CE25537 /TR=Q9N305 /GB=AAF60850.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 17      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 5.6
(-3)
A 		47.6
(1.5)
A 		9.6
(-2.6)
A 		61.4
(3)
A 		56
B 3.7
(-3)
A 		4.3
(-2.9)
A 		9.9
(-2)
A 		41
(3)
A 		37
C 12.2
(-3)
A 		13.1
(-2.9)
A 		21.2
(-2.5)
A 		119.8
(3)
A 		108
avr 7.2
(-3) 		21.7
(-1.7) 		13.6
(-2.4) 		74.1
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 12.4
(-2.8)
A 		18.1
(-2.7)
A 		7.2
(-3)
A 		37.7
(-2.1)
A 		146
(1)
A 		213.8
(3)
M 		207
B 5.3
(-3)
A 		6.2
(-3)
A 		47.7
(-1.2)
A 		40.5
(-1.5)
A 		130.1
(2.3)
A 		147.5
(3)
P 		142
C 6.2
(-2.9)
A 		6.8
(-2.9)
A 		4.2
(-3)
A 		63
(-1.3)
A 		162.4
(1.5)
A 		216.7
(3)
P 		213
avr 8
(-3) 		10.4
(-2.9) 		19.7
(-2.6) 		47.1
(-1.7) 		146.2
(1.5) 		192.7
(3) 		185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 2
(-3)
A 		10.3
(3)
A 		2.5
(-2.6)
A 		9
(2.1)
A 		8
B 1.2
(-2.9)
A 		1.1
(-3)
A 		2.3
(-2.1)
A 		8.7
(3)
A 		8
C 4
(-2.9)
A 		3.8
(-3)
A 		7.4
(-1.7)
A 		20.2
(3)
A 		16
avr 2.4
(-3) 		5.1
(-1.4) 		4.1
(-2) 		12.6
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 2.3
(-2.9)
A 		6.4
(-2.4)
A 		1.6
(-3)
A 		9.6
(-2)
A 		44.1
(2.1)
A 		52
(3)
M 		50
B 1.9
(-3)
A 		2.1
(-3)
A 		11.6
(-1.9)
A 		10.7
(-2)
A 		52.9
(2.8)
A 		55
(3)
P 		53
C 2.3
(-2.9)
A 		2.1
(-2.9)
A 		1.1
(-3)
A 		12.7
(-2)
A 		52.5
(1.3)
A 		72.5
(3)
P 		71
avr 2.2
(-3) 		3.5
(-2.9) 		4.8
(-2.7) 		11
(-2.1) 		49.8
(2) 		59.8
(3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186874_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2564
Sequence Type Exemplar sequence
Representative Public ID Y65B4BL.3 NCBI
Target Description Y65B4BL.3 /REP_DB=WormBase Gene ID /WP=CE25537 /TR=Q9N305 /GB=AAF60850.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058339(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058339 NCBI Caenorhabditis elegans Y65B4BL.3 (Y65B4BL.3) mRNA, complete cds. 11/11 None
SNAP00000040874 ENSEMBL cdna:SNAP chromosome:CEL140:I:496233:502022:-1 10/11 None
Y65B4BL.3 ENSEMBL cdna:known chromosome:CEL140:I:496208:502046:-1 gene:Y65B4BL.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040890 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:496232-502033(-) 88.01 100.0
Public Domain and Genome References
Gene Title
Ensembl Y65B4BL.3
Entrez Gene 171640 Entrez gene
SwissProt Q9N305 EMBL-EBI
WormBase CE34420 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60850 Hypothetical protein Y65B4BL.3 [Caenorhabditis elegans] ref|NP_490740.2| Y65B4BL.3 [Caenorhabditis elegans] 0.0
blast AAF60850 Hypothetical protein Y65B4BL.3 [Caenorhabditis elegans] ref|NP_490740.2| Y65B4BL.3 [Caenorhabditis elegans] 1.0E-135
blast CAE74829 Hypothetical protein CBG22667 [Caenorhabditis briggsae] 1.0E-93
blast CAE74829 Hypothetical protein CBG22667 [Caenorhabditis briggsae] 4.0E-93
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490740.2 5 101-120,133-155,175-197,209-231,241-263
Y65B4BL.3 5 101-120,133-155,175-197,209-231,241-263
SNAP00000040874 4 34-56,105-124,137-159,174-196
Sequence
Target Sequence 
>C. ELEGANS:186874_S_AT
aatttgccacgtttcatgatttcgggcgaatttttcgtggaaaagtgtgcatatctgctc
attttcttcttcatcagctgcacgatgtttctgtatgcggtgtatttggtgaaacatgag
actgagctgaagattcgatatcgagcgattttgacgttgcccagcatcttgaaatggcga
atttcgtttgcgcctaagaattttcttcnnnnnnnnnnnnnnnnnnnnnncgccgaattc
aatttttatcagccagcttcattagtttttgtcgattttggttttttttcaccgttagtg
atccactttgccacattgtactacattatgatctacccggttattccactgtcgatttac
acagtatcgaatgcgaattactcgacactgaactacgggcagcgaggcggaagtggaaga
agcaatcattatgagactctaaacgccggtccactgttgagcgactcgtacaggctgtct
gtgaataattggcaagctgccaacgagacacgtggcaccgtaagctcctactctcaaatc
cgcggatggaacaacacgatccggtcgacgaacacgatgcggc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTTGCCACGTTTCATGATTTCGG 309 173 265 Antisense
GTGTGCATATCTGCTCATTTTCTTC 28 489 309 Antisense
CAGCCAGCTTCATTAGTTTTTGTCG 309 203 514 Antisense
TGATCCACTTTGCCACATTGTACTA 581 565 563 Antisense
ATGATCTACCCGGTTATTCCACTGT 158 45 592 Antisense
ATTACTCGACACTGAACTACGGGCA 213 3 641 Antisense
TAAACGCCGGTCCACTGTTGAGCGA 352 639 704 Antisense
GCGACTCGTACAGGCTGTCTGTGAA 470 289 725 Antisense
ACGAGACACGTGGCACCGTAAGCTC 613 91 767 Antisense
TCCTACTCTCAAATCCGCGGATGGA 691 591 790 Antisense
ATCCGGTCGACGAACACGATGCGGC 359 39 823 Antisense
Sequence Source Affymetrix Proprietary Database