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[186872_s_at]

Description T21D12.9A /REP_DB=WormBase Gene ID /WP=CE28415 /TR=O16779 /GB=AAC48096.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 73      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 64      6grp_total > 74      4grp_total > 14      6grp_high1_sub > 03_04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 835
1 2 3 4 range
A 709.6
(0.9)
P 		290.9
(-3)
P 		321
(-2.7)
P 		930.3
(3)
P 		639
B 768
(0.5)
P 		391.6
(-2.2)
P 		277.1
(-3)
P 		1112.2
(3)
P 		835
C 907
(1.8)
P 		350
(-2.8)
P 		329.3
(-3)
P 		1045.3
(3)
P 		716
avr 794.9
(1) 		344.2
(-2.7) 		309.1
(-3) 		1029.3
(3) 		720

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 582
1 2 3 4 5 6 range
A 488.6
(-3)
P 		837.5
(0.9)
P 		793.3
(0.4)
P 		1032.3
(3)
P 		802.8
(0.5)
P 		891.4
(1.4)
P 		544
B 582.8
(-3)
P 		778.4
(-0.4)
P 		885.9
(1)
P 		1034.9
(3)
P 		677.2
(-1.7)
P 		856.9
(0.6)
P 		452
C 546.6
(-3)
P 		702.4
(-1.2)
P 		888.4
(0.9)
P 		1071
(3)
P 		677.6
(-1.5)
P 		940.5
(1.5)
P 		524
avr 539.3
(-3) 		772.8
(-0.2) 		855.9
(0.7) 		1046.1
(3) 		719.2
(-0.9) 		896.3
(1.2) 		507
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 237
1 2 3 4 range
A 258.4
(3)
P 		63.1
(-3)
P 		83.9
(-2.4)
P 		136.2
(-0.8)
P 		195
B 244.9
(3)
P 		96.7
(-1.9)
P 		64.2
(-3)
P 		237
(2.7)
P 		181
C 300.2
(3)
P 		100.6
(-3)
P 		114.8
(-2.6)
P 		176.2
(-0.7)
P 		200
avr 267.8
(3) 		86.8
(-3) 		87.6
(-3) 		183.1
(0.2) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 230
1 2 3 4 5 6 range
A 90.1
(-3)
P 		297.8
(3)
P 		172.8
(-0.6)
P 		261.8
(2)
P 		242.2
(1.4)
P 		217
(0.7)
P 		208
B 202.8
(-3)
P 		259.1
(-0.1)
P 		215.1
(-2.4)
P 		274.1
(0.7)
P 		275.1
(0.7)
P 		319.6
(3)
P 		117
C 202.6
(-3)
P 		214.3
(-2.4)
P 		226.1
(-1.7)
P 		215.4
(-2.3)
P 		219.3
(-2.1)
P 		314.8
(3)
P 		112
avr 165.2
(-3) 		257.1
(1.6) 		204.7
(-1) 		250.4
(1.3) 		245.5
(1.1) 		283.8
(3) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186872_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11147
Sequence Type Exemplar sequence
Representative Public ID T21D12.9A NCBI
Target Description T21D12.9A /REP_DB=WormBase Gene ID /WP=CE28415 /TR=O16779 /GB=AAC48096.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067495(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067495 NCBI Caenorhabditis elegans T21D12.9a (T21D12.9) mRNA, complete cds. 11/11 A 
GENEFINDER00000019195 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:274626:279815:1 11/11 None
T21D12.9a ENSEMBL cdna:known chromosome:CEL140:IV:274626:279938:1 gene:T21D12.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019189 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176849 3 1 NM_067494 186885_at (11)
NM_067495 174086_s_at (11), 186872_s_at (11)
NM_067496 174086_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:275502-279815(+) 100.0 100.0
Public Domain and Genome References
Ensembl T21D12.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK72063 Hypothetical protein T21D12.9a [Caenorhabditis elegans] ref|NP_499896.1| T21D12.9a [Caenorhabditis elegans] 0.0
blast T28714 hypothetical protein T21D12.9a - Caenorhabditis elegans 0.0
blast T28715 hypothetical protein T21D12.9b - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0017
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.092
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.014
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.16
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0080
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.15
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0013
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 7.7E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.036
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0068
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0024
Pfam IPR000372 EMBL-EBI Cysteine-rich flanking region, N-terminal 1.4E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 7.1E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.0E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499896.1 1 839-861
T21D12.9a 1 839-861
GENEFINDER00000019195 1 839-861
Sequence
Target Sequence 
>C. ELEGANS:186872_S_AT
aaagccctgatttaagcccgatgctgattaaaaaaagagacgcattaaaaatagattgct
cttgtgatcttatttccagtcgacagagaatggtgtggaaacggcagcaacaagttatac
ttttcttaaagaaagcaaagttctcagacaatgatcaaattctgactctaacccagacga
cgttctctgattctggagaatactcgtgtgaattatgggttgatgatacgatactgatga
gaaaaattacaatggtgaaggtggtaaacgaagatgaatttgtttcttcggaagccacct
taactcaacgtgtacaacgcatccgagcaatggcctcaaaattagtagataatatcaaat
ctacagggaatggaatttatctcatggcttcttgtagtgcatttggactaatgattctgg
gagttatcacttcaatttgcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCCCTGATTTAAGCCCGATGCT 591 123 1877 Antisense
GCTCTTGTGATCTTATTTCCAGTCG 377 301 1934 Antisense
AGAATGGTGTGGAAACGGCAGCAAC 391 75 1963 Antisense
ATTCTGACTCTAACCCAGACGACGT 150 5 2035 Antisense
CAGACGACGTTCTCTGATTCTGGAG 679 205 2050 Antisense
CGGAAGCCACCTTAACTCAACGTGT 656 241 2165 Antisense
CTCAACGTGTACAACGCATCCGAGC 259 227 2180 Antisense
GCATCCGAGCAATGGCCTCAAAATT 605 307 2195 Antisense
GGAATTTATCTCATGGCTTCTTGTA 414 533 2248 Antisense
TGGCTTCTTGTAGTGCATTTGGACT 688 545 2261 Antisense
GGGAGTTATCACTTCAATTTGCATT 192 495 2295 Antisense
Sequence Source Affymetrix Proprietary Database