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[186857_at]

Description Y116A8C.12 /REP_DB=WormBase Gene ID /WP=CE24147 /TR=Q9U2S4 /GB=CAB55153.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 65      4grp_high_8_8 > 05      6grp_highest_nonorm > 82      6grp_total > 55      4grp_total > 11      6grp_cgc > 46      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 389
1 2 3 4 range
A 258.2
(-2.5)
P 		382.6
(0.5)
P 		236.6
(-3)
M 		483.6
(3)
P 		247
B 394.7
(-0.5)
P 		229.7
(-3)
P 		273.1
(-2.3)
P 		618.3
(3)
P 		389
C 306
(-2.9)
P 		305.7
(-3)
P 		319.6
(-0.2)
P 		335.3
(3)
P 		30
avr 319.6
(-1.7) 		306
(-2.1) 		276.4
(-3) 		479.1
(3) 		203

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 453
1 2 3 4 5 6 range
A 335.7
(0)
P 		217.9
(-3)
P 		450.5
(3)
P 		268.2
(-1.7)
P 		276.1
(-1.5)
P 		282.3
(-1.3)
P 		233
B 420.1
(0.5)
P 		302.4
(-1.4)
P 		452.5
(1)
P 		349.8
(-0.6)
P 		199
(-3)
P 		578.8
(3)
P 		380
C 126
(-3)
P 		303.4
(-0.4)
P 		247.1
(-1.2)
P 		221.5
(-1.6)
P 		175.4
(-2.3)
P 		538.3
(3)
P 		412
avr 293.9
(-1.1) 		274.6
(-1.6) 		383.4
(1) 		279.8
(-1.5) 		216.8
(-3) 		466.5
(3) 		250
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 94
(3)
P 		83.1
(1)
P 		61.9
(-3)
M 		70.8
(-1.3)
P 		32
B 125.9
(2.5)
P 		56.7
(-3)
P 		63.3
(-2.5)
P 		131.7
(3)
P 		75
C 101.3
(1.9)
P 		87.9
(0.4)
P 		111.4
(3)
P 		56.5
(-3)
P 		55
avr 107.1
(3) 		75.9
(-3) 		78.9
(-2.4) 		86.3
(-1) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 61.9
(-3)
P 		77.5
(-0.4)
P 		98.1
(3)
P 		68
(-2)
P 		83.3
(0.5)
P 		68.7
(-1.9)
P 		36
B 146.2
(-0.1)
P 		100.7
(-2.1)
P 		109.9
(-1.7)
P 		92.7
(-2.5)
P 		80.9
(-3)
P 		215.8
(3)
P 		135
C 46.7
(-2.9)
P 		92.6
(-0.9)
P 		62.9
(-2.2)
P 		44.6
(-3)
P 		56.8
(-2.5)
P 		180.2
(3)
P 		136
avr 84.9
(-1.9) 		90.3
(-1.5) 		90.3
(-1.5) 		68.4
(-3) 		73.7
(-2.6) 		154.9
(3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186857_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10263
Sequence Type Exemplar sequence
Representative Public ID Y116A8C.12 NCBI
Target Description Y116A8C.12 /REP_DB=WormBase Gene ID /WP=CE24147 /TR=Q9U2S4 /GB=CAB55153.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070610(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070610 NCBI Caenorhabditis elegans ADP-Ribosylation Factor related family member (arf-6) (arf-6) mRNA, complete cds. 11/11 None
GENEFINDER00000004184 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16947086:16948484:1 9/11 None
Y116A8C.12.1 ENSEMBL cdna:known chromosome:CEL140:IV:16947086:16949541:1 gene:Y116A8C.12 11/11 None
Y116A8C.12.2 ENSEMBL cdna:known chromosome:CEL140:IV:16947086:16949506:1 gene:Y116A8C.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004080 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16947091-16949425(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol arf-6
Ensembl Y116A8C.12
Entrez Gene 191608 Entrez gene
SwissProt Q9U2S4 EMBL-EBI
WormBase CE24147 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603859_AT similar to ADP-ribosylation factor 6 cfa
CANINE_2:CFAAFFX.21943.1.S1_AT similar to ADP-ribosylation factor 6 cfa
CANINE_2:CFAAFFX.21943.1.S1_S_AT similar to ADP-ribosylation factor 6 cfa
DROSOPHILA_2:1624208_S_AT ADP ribosylation factor 51F dm
DROSGENOME1:143716_AT ADP ribosylation factor 51F dm
CHICKEN:GGA.2511.1.S1_AT ADP-ribosylation factor 6 gga
HG-U133_PLUS_2:203311_S_AT ADP-ribosylation factor 6 hs
HG-U133A:203311_S_AT ADP-ribosylation factor 6 hs
HG-U133A_2:203311_S_AT ADP-ribosylation factor 6 hs
HG-U133_PLUS_2:203312_X_AT ADP-ribosylation factor 6 hs
HG-U133A:203312_X_AT ADP-ribosylation factor 6 hs
HG-U133A_2:203312_X_AT ADP-ribosylation factor 6 hs
HU35KSUBA:RC_AA291260_AT ADP-ribosylation factor 6 hs
HU35KSUBD:RC_AA237077_AT ADP-ribosylation factor 6 hs
HUGENEFL:M57763_AT ADP-ribosylation factor 6 hs
U133_X3P:G6996000_3P_A_AT ADP-ribosylation factor 6 hs
U133_X3P:G178988_3P_A_AT ADP-ribosylation factor 6 hs
U133_X3P:HS.180777.0.A1_3P_AT ADP-ribosylation factor 6 hs
HG-U133_PLUS_2:224788_AT ADP-ribosylation factor 6 hs
HG-U133B:224788_AT ADP-ribosylation factor 6 hs
HG-U95AV2:33152_AT ADP-ribosylation factor 6 hs
HG-U95B:43480_AT ADP-ribosylation factor 6 hs
HG-U95B:54829_S_AT ADP-ribosylation factor 6 hs
HG-U95B:54831_R_AT ADP-ribosylation factor 6 hs
HG-U95AV2:37984_S_AT ADP-ribosylation factor 6 hs
HG-U133A_2:214182_AT ADP-ribosylation factor 6 hs
HG-FOCUS:214182_AT ADP-ribosylation factor 6 hs
HG-U133_PLUS_2:214182_AT ADP-ribosylation factor 6 hs
HG-U133A:214182_AT ADP-ribosylation factor 6 hs
U133_X3P:HS.89474.3.A1_3P_AT ADP-ribosylation factor 6 hs
MG-U74AV2:100462_AT ADP-ribosylation factor 6 mm
MU11KSUBA:D87903_S_AT ADP-ribosylation factor 6 mm
MOE430A:1418822_A_AT ADP-ribosylation factor 6 mm
MOUSE430A_2:1418822_A_AT ADP-ribosylation factor 6 mm
MOUSE430_2:1418822_A_AT ADP-ribosylation factor 6 mm
MOE430A:1418824_AT ADP-ribosylation factor 6 mm
MOUSE430A_2:1418824_AT ADP-ribosylation factor 6 mm
MOUSE430_2:1418824_AT ADP-ribosylation factor 6 mm
MOE430A:1418823_AT ADP-ribosylation factor 6 mm
MOUSE430A_2:1418823_AT ADP-ribosylation factor 6 mm
MOUSE430_2:1418823_AT ADP-ribosylation factor 6 mm
MU11KSUBA:AA174512_S_AT ADP-ribosylation factor 6, mRNA (cDNA clone MGC:5936 IMAGE:2655889) mm
RAE230A:1369998_AT ADP-ribosylation factor 6 rn
RAT230_2:1369998_AT ADP-ribosylation factor 6 rn
RG-U34A:RC_AA944324_AT ADP-ribosylation factor 6 rn
RAE230A:1376268_AT ADP-ribosylation factor 6 rn
RAT230_2:1376268_AT ADP-ribosylation factor 6 rn
RG-U34B:RC_AI044614_AT ADP-ribosylation factor 6 rn
RG-U34C:RC_AI146036_AT ADP-ribosylation factor 6 rn
YEAST_2:1770423_AT Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB55153 Hypothetical protein Y116A8C.12 [Caenorhabditis elegans] ref|NP_503011.1| ADP-Ribosylation Factor related family member (arf-6) [Caenorhabditis elegans] 1.0E-87
blast CAE57387 Hypothetical protein CBG00335 [Caenorhabditis briggsae] 2.0E-87
blast AAH64293 Unknown (protein for MGC:77665) [Danio rerio] ref|NP_956287.1| hypothetical protein LOC336063 [Danio rerio] 2.0E-79
blast CAB55153 Hypothetical protein Y116A8C.12 [Caenorhabditis elegans] ref|NP_503011.1| ADP-Ribosylation Factor related family member (arf-6) [Caenorhabditis elegans] 4.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 1.0E-116
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 4.4E-97
Sequence
Target Sequence 
>C. ELEGANS:186857_AT
attaatgctcggactcgatgcggcgggtaaaaccacaattctgtacaaactgaagctcgg
gcaatcagtgaccacaattccgacggtgggcttcaatgtggagactgtcacgtataaaaa
tatcaaattcaacgtttgggacgtcggcggacaggacaaaattcgacccctctggcgaca
ttattacacaggaactcaggcgctcatttttgtgatggacgccgctgatagagacagagt
cgacgaggcgcgaatggagcttcaccgaataattaatgatcgggaaatgaaggaggcgat
tattttggtttttgccaataaacaggatcttgccgatgcgatgaaaccacacgaaattca
ggataaattgggattgacgaggattcgagacaggaattggtacgtccaaccatcatgtgc
ttcaaccggagacggcctacacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTAATGCTCGGACTCGATGCGGCG 139 5 60 Antisense
GAAGCTCGGGCAATCAGTGACCACA 309 343 111 Antisense
GACCACAATTCCGACGGTGGGCTTC 27 381 129 Antisense
GGTGGGCTTCAATGTGGAGACTGTC 333 499 144 Antisense
GACAGGACAAAATTCGACCCCTCTG 238 367 209 Antisense
TTACACAGGAACTCAGGCGCTCATT 566 681 243 Antisense
GTGATGGACGCCGCTGATAGAGACA 106 485 271 Antisense
CGACGAGGCGCGAATGGAGCTTCAC 582 251 300 Antisense
GGATCTTGCCGATGCGATGAAACCA 364 511 384 Antisense
TACGTCCAACCATCATGTGCTTCAA 485 647 460 Antisense
CTTCAACCGGAGACGGCCTACACGA 144 217 479 Antisense
Sequence Source Affymetrix Proprietary Database