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[186853_s_at]

Description C05B5.3 /REP_DB=WormBase Gene ID /WP=CE17369 /TR=SW:P34291 /GB=CAA83596.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 0      6grp_highest_nonorm > 90      6grp_total > 03      4grp_total > 46      6grp_cgc > 50      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 235
1 2 3 4 range
A 304.8
(3)
P 		183.3
(-0.1)
P 		162.4
(-0.6)
M 		69.5
(-3)
A 		235
B 292.4
(3)
P 		98.6
(-3)
A 		129
(-2.1)
A 		150.5
(-1.4)
A 		194
C 279.3
(3)
P 		179.1
(-0.9)
M 		124.1
(-3)
P 		156.2
(-1.8)
A 		155
avr 292.2
(3) 		153.7
(-2) 		138.5
(-2.5) 		125.4
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 224.2
(3)
P 		219
(2.8)
P 		148.3
(-0.6)
P 		176.1
(0.7)
P 		138.7
(-1)
P 		97.1
(-3)
P 		127
B 212.1
(3)
P 		171.8
(0.6)
P 		179.3
(1)
P 		194.8
(2)
P 		199.8
(2.3)
P 		112.2
(-3)
P 		100
C 214.4
(0.7)
P 		190
(-0.6)
P 		171.7
(-1.5)
P 		257.3
(3)
P 		143.8
(-3)
P 		150.8
(-2.6)
P 		114
avr 216.9
(3) 		193.6
(1.6) 		166.4
(-0.1) 		209.4
(2.5) 		160.8
(-0.5) 		120
(-3) 		97
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 111
(3)
P 		39.8
(-1.2)
P 		42.5
(-1.1)
M 		10.2
(-3)
A 		101
B 93.2
(3)
P 		24.4
(-3)
A 		29.9
(-2.5)
A 		32.1
(-2.3)
A 		69
C 92.5
(3)
P 		51.5
(-0.7)
M 		43.3
(-1.5)
P 		26.3
(-3)
A 		66
avr 98.9
(3) 		38.6
(-1.8) 		38.6
(-1.8) 		22.9
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 41.3
(-1)
P 		77.9
(3)
P 		32.3
(-2)
P 		44.7
(-0.7)
P 		41.9
(-1)
P 		23.6
(-3)
P 		54
B 73.8
(1.9)
P 		57.2
(-0.7)
P 		43.5
(-2.7)
P 		51.6
(-1.5)
P 		81.2
(3)
P 		41.8
(-3)
P 		39
C 79.5
(3)
P 		58
(-0.6)
P 		43.7
(-3)
P 		51.7
(-1.7)
P 		46.5
(-2.5)
P 		50.5
(-1.9)
P 		36
avr 64.9
(3) 		64.4
(2.9) 		39.8
(-2.7) 		49.3
(-0.6) 		56.5
(1.1) 		38.6
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186853_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7055
Sequence Type Exemplar sequence
Representative Public ID C05B5.3 NCBI
Target Description C05B5.3 /REP_DB=WormBase Gene ID /WP=CE17369 /TR=SW:P34291 /GB=CAA83596.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066816(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066816 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-8) (pqn-8) mRNA, complete cds. 11/11 None
C05B5.3 ENSEMBL cdna:known chromosome:CEL140:III:9996154:9997811:-1 gene:C05B5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026741 8/11 Cross Hyb Matching Probes None
GENEFINDER00000026765 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9996149-9997807(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pqn-8
Ensembl C05B5.3
Entrez Gene 176411 Entrez gene
SwissProt P34291 EMBL-EBI
WormBase CE17369 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83596 Hypothetical protein C05B5.3 [Caenorhabditis elegans] ref|NP_499217.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-8) [Caenorhabditis elegans] sp|P34291|PQN8_CAEEL Protein pqn-8 2.0E-94
blast S43574 C05B5.3 protein (clone C05B5) - Caenorhabditis elegans 2.0E-59
blast CAE65073 Hypothetical protein CBG09927 [Caenorhabditis briggsae] 2.0E-19
Sequence
Target Sequence 
>C. ELEGANS:186853_S_AT
caacatccgggttctctaaccaacaaacttctaatcaaaactctggatttttcaataatc
aaaaccaacaaaacggacaaaattttggaaactctgggaatcaaaatggagtcaatccct
attctggagcattttcaaacgggcaaaatcaaaatcaacaaggattctttggaaataatc
aaaataatcaacaaaattccaatggtcaagttcaaggatcacaaaataatcaaatttgga
atcaaaatcaaaacccaaatattttaccatttggacccaatctagtgaacagtaatacac
agttcgggccacaaccgtttcaaccaatccaggttggctctattcgtcaaccattttcaa
acaaggactggaattttcagcccggcggtcaagctgttcaatttgtgggaggaggtggaa
gtgggggagcaccgggatcgcaatccgtaccgatgactgatgaagcacagcagattgctg
ttcaaattcaggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACATCCGGGTTCTCTAACCAACA 176 189 620 Antisense
GGAGTCAATCCCTATTCTGGAGCAT 599 517 727 Antisense
TCTGGAGCATTTTCAAACGGGCAAA 366 593 742 Antisense
ATATTTTACCATTTGGACCCAATCT 15 19 878 Antisense
TACACAGTTCGGGCCACAACCGTTT 379 641 915 Antisense
CACAACCGTTTCAACCAATCCAGGT 476 191 929 Antisense
CAATCCAGGTTGGCTCTATTCGTCA 139 183 944 Antisense
TGGCTCTATTCGTCAACCATTTTCA 592 545 954 Antisense
GTGGAAGTGGGGGAGCACCGGGATC 553 491 1034 Antisense
TCGCAATCCGTACCGATGACTGATG 395 599 1057 Antisense
GCAGATTGCTGTTCAAATTCAGGCA 80 313 1089 Antisense
Sequence Source Affymetrix Proprietary Database