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[186851_at]

Description B0285.5 /REP_DB=WormBase Gene ID /WP=CE00644 /TR=SW:P46555 /GB=CAA84297.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 76      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 81      6grp_total > 76      4grp_total > 23      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 269
(3)
P 		173.2
(-3)
P 		215.3
(-0.4)
P 		259.7
(2.4)
P 		96
B 179
(0.4)
P 		198.8
(3)
P 		153.2
(-3)
P 		188.6
(1.7)
P 		46
C 323.7
(3)
P 		213.1
(-3)
P 		256.9
(-0.6)
P 		257.9
(-0.6)
P 		111
avr 257.2
(3) 		195
(-3) 		208.5
(-1.7) 		235.4
(0.9) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 177.7
(-2.7)
P 		294.9
(2)
P 		169.1
(-3)
P 		272.6
(1.1)
P 		281.5
(1.4)
P 		321.1
(3)
P 		152
B 209.5
(-2.4)
P 		282.9
(1.3)
P 		197.1
(-3)
P 		272.4
(0.8)
P 		227.2
(-1.5)
P 		317.2
(3)
P 		120
C 272.5
(-0)
P 		244.6
(-1.8)
P 		269.1
(-0.2)
P 		310.4
(2.4)
P 		225.4
(-3)
P 		319.9
(3)
P 		95
avr 219.9
(-2.5) 		274.1
(0.5) 		211.8
(-3) 		285.1
(1.1) 		244.7
(-1.2) 		319.4
(3) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 97.9
(3)
P 		37.6
(-3)
P 		56.3
(-1.1)
P 		38
(-3)
P 		60
B 57.1
(3)
P 		49.1
(0.8)
P 		35.5
(-3)
P 		40.2
(-1.7)
P 		22
C 107.2
(3)
P 		61.3
(-1.3)
P 		89.5
(1.3)
P 		43.5
(-3)
P 		64
avr 87.4
(3) 		49.3
(-1.9) 		60.4
(-0.5) 		40.6
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 32.7
(-3)
P 		104.9
(3)
P 		36.8
(-2.7)
P 		69.1
(0)
P 		84.9
(1.3)
P 		78.2
(0.8)
P 		72
B 72.9
(-0.9)
P 		94.2
(0.9)
P 		47.9
(-3)
P 		72.1
(-0.9)
P 		92.3
(0.8)
P 		118.3
(3)
P 		70
C 101
(2.2)
P 		74.6
(-1.4)
P 		68.5
(-2.2)
P 		62.4
(-3)
P 		73
(-1.6)
P 		107.1
(3)
P 		45
avr 68.9
(-0.9) 		91.2
(1.8) 		51.1
(-3) 		67.9
(-1) 		83.4
(0.9) 		101.2
(3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186851_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7269
Sequence Type Exemplar sequence
Representative Public ID B0285.5 NCBI
Target Description B0285.5 /REP_DB=WormBase Gene ID /WP=CE00644 /TR=SW:P46555 /GB=CAA84297.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065475(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065475 NCBI Caenorhabditis elegans Heparan Sulfate-glucuronic acid-5-Epimerase family member (hse-5) (hse-5) mRNA, complete cds. 11/11 None
SNAP00000026084 ENSEMBL cdna:SNAP chromosome:CEL140:III:4345311:4349713:1 11/11 None
GENEFINDER00000026094 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4345311:4348962:1 11/11 None
B0285.5 ENSEMBL cdna:known chromosome:CEL140:III:4345308:4350027:1 gene:B0285.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4345221-4348962(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hse-5
Ensembl B0285.5
Entrez Gene 175562 Entrez gene
SwissProt P46555 EMBL-EBI
WormBase CE36681 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1602937_AT similar to D-glucuronyl C5-epimerase cfa
CANINE_2:CFA.6771.1.S1_AT similar to D-glucuronyl C5-epimerase cfa
CANINE_2:CFAAFFX.26782.1.S1_AT similar to D-glucuronyl C5-epimerase cfa
CANINE_2:CFAAFFX.26782.1.S1_S_AT similar to D-glucuronyl C5-epimerase cfa
DROSOPHILA_2:1628914_AT dm
DROSGENOME1:146683_AT dm
CHICKEN:GGAAFFX.5062.1.S1_AT similar to D-glucuronyl C5 epimerase gga
HG-U133_PLUS_2:213552_AT glucuronyl C5-epimerase hs
HG-U133A_2:213552_AT glucuronyl C5-epimerase hs
HG-U133A:213552_AT glucuronyl C5-epimerase hs
HU35KSUBA:RC_AA461463_AT glucuronyl C5-epimerase hs
U133_X3P:HS.183006.0.S1_3P_X_AT glucuronyl C5-epimerase hs
U133_X3P:HS.183006.0.S1_3P_AT glucuronyl C5-epimerase hs
HG-U95AV2:33296_AT glucuronyl C5-epimerase hs
MOE430A:1425876_A_AT glucuronyl C5-epimerase mm
MOUSE430A_2:1425876_A_AT glucuronyl C5-epimerase mm
MOUSE430_2:1425876_A_AT glucuronyl C5-epimerase mm
MU11KSUBA:AA290533_AT glucuronyl C5-epimerase mm
MG-U74AV2:103739_AT glucuronyl C5-epimerase mm
MOE430A:1428374_AT glucuronyl C5-epimerase mm
MOUSE430A_2:1428374_AT glucuronyl C5-epimerase mm
MOUSE430_2:1428374_AT glucuronyl C5-epimerase mm
MOE430B:1440386_AT glucuronyl C5-epimerase mm
MOUSE430_2:1440386_AT glucuronyl C5-epimerase mm
MOE430B:1459654_AT Glucuronyl C5-epimerase (Glce), mRNA mm
MOUSE430_2:1459654_AT Glucuronyl C5-epimerase (Glce), mRNA mm
MU19KSUBC:TC38320_AT Glucuronyl C5-epimerase (Glce), mRNA mm
RAE230A:1390385_AT Similar to D-glucuronyl C5-epimerase rn
RAT230_2:1390385_AT Similar to D-glucuronyl C5-epimerase rn
RG-U34B:RC_AI012231_AT Similar to D-glucuronyl C5-epimerase rn
Protein Similarities
Method ID Description E-Value
blast CAA84297 Hypothetical protein B0285.5 [Caenorhabditis elegans] ref|NP_497876.2| Heparan Sulfate-glucuronic acid-5-Epimerase family member (hse-5) [Caenorhabditis elegans] gb|AAS89254.1| heparan sulfate C5-glucuronyl epimerase [Caenorhabditis elegans] sp|P46555|GLCE_CAEEL D-glucuronyl C5 epimerase (Heparin/heparan sulfate:glucuronic acid C5 epimerase) 0.0
blast CAE71222 Hypothetical protein CBG18086 [Caenorhabditis briggsae] 0.0
blast XP_393602 PREDICTED: similar to ENSANGP00000016480 [Apis mellifera] 1.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR010598 EMBL-EBI D-glucuronyl C5-epimerase, C-terminal 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497876.2 1 13-30
B0285.5 1 13-30
SNAP00000026084 1 13-30
GENEFINDER00000026094 1 13-30
Sequence
Target Sequence 
>C. ELEGANS:186851_AT
cgaggaggatggtcggttcctgtggaaagatcaattgccgagagaaagttggttttacca
cccggatggcacagtgctatggctcaaggacacggaatatcagttctaactcgtgcattc
aaacatttcaacgatgaaaaatatttgaaatctgcagcaaaagctctgaaactattcaaa
attaactcatcagacggcggtgttcgtggagaattttttggaaatatttggtatgaggaa
tatccaacaacaccgggatcttttgtgctcaatggattcctttattcacttattggactt
tatgatctttcacaattggaattgatgattgatgaaaatgatgaaacaatgcgggcgaaa
attcaagaggcacaagaattatattcagcaggtgtccgatcactgaaacaacttctccct
ttatatgatactggtagtggaacgatttatgatcttcgtcacgttgctttgggaactgca
ccgaatcttgcaagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAGGAGGATGGTCGGTTCCTGTGG 378 249 1288 Antisense
GGCACAGTGCTATGGCTCAAGGACA 75 533 1355 Antisense
ATCAGTTCTAACTCGTGCATTCAAA 443 29 1386 Antisense
AACTCATCAGACGGCGGTGTTCGTG 361 141 1471 Antisense
ACCGGGATCTTTTGTGCTCAATGGA 311 87 1539 Antisense
AATGGATTCCTTTATTCACTTATTG 157 163 1558 Antisense
GCAGGTGTCCGATCACTGAAACAAC 441 311 1675 Antisense
GAAACAACTTCTCCCTTTATATGAT 707 355 1692 Antisense
GATTTATGATCTTCGTCACGTTGCT 272 429 1731 Antisense
GTCACGTTGCTTTGGGAACTGCACC 628 465 1745 Antisense
GGAACTGCACCGAATCTTGCAAGAT 73 531 1759 Antisense
Sequence Source Affymetrix Proprietary Database