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[186842_at]

Description Y77E11A.6 /REP_DB=WormBase Gene ID /WP=CE25620 /TR=Q9N4B7 /GB=AAF36076.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 76      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 83      6grp_total > 78      4grp_total > 01      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2064
1 2 3 4 range
A 391.5
(-3)
P 		588
(-2.2)
P 		597.9
(-2.2)
P 		1862
(3)
P 		1471
B 477.4
(-2.6)
P 		354.9
(-3)
P 		580.4
(-2.3)
P 		2419.2
(3)
P 		2064
C 494.3
(-2.7)
P 		406.7
(-3)
P 		530.6
(-2.6)
P 		2146.6
(3)
P 		1740
avr 454.4
(-3) 		449.9
(-3) 		569.6
(-2.6) 		2142.6
(3) 		1693

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 377.7
(-3)
P 		476
(0.2)
P 		460.1
(-0.3)
P 		408.7
(-2)
P 		471.7
(0)
P 		563.4
(3)
P 		186
B 424.3
(-2.9)
P 		467
(-1.3)
P 		517.6
(0.5)
P 		421.4
(-3)
P 		537.7
(1.3)
P 		585.1
(3)
P 		164
C 507.1
(-0.6)
P 		457.1
(-1.9)
P 		469.3
(-1.6)
P 		414.3
(-3)
P 		455.8
(-1.9)
P 		650
(3)
P 		236
avr 436.4
(-2.3) 		466.7
(-1.3) 		482.3
(-0.8) 		414.8
(-3) 		488.4
(-0.6) 		599.5
(3) 		185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 428
1 2 3 4 range
A 142.5
(-2.4)
P 		127.6
(-3)
P 		156.3
(-1.8)
P 		272.6
(3)
P 		145
B 152.2
(-2.1)
P 		87.7
(-3)
P 		134.5
(-2.3)
P 		515.5
(3)
P 		428
C 163.6
(-1.9)
P 		116.9
(-3)
P 		184.9
(-1.3)
P 		362
(3)
P 		245
avr 152.8
(-2.1) 		110.7
(-3) 		158.6
(-1.9) 		383.4
(3) 		273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 69.6
(-3)
P 		169.3
(3)
P 		100.2
(-1.2)
P 		103.7
(-0.9)
P 		142.3
(1.4)
P 		137.2
(1.1)
P 		100
B 147.6
(-1)
P 		155.5
(-0.5)
P 		125.7
(-2.2)
P 		111.6
(-3)
P 		218.5
(3)
P 		218.2
(3)
P 		107
C 188
(1.7)
P 		139.5
(-0.5)
P 		119.5
(-1.4)
P 		83.3
(-3)
P 		147.5
(-0.1)
P 		217.6
(3)
P 		134
avr 135.1
(-0.7) 		154.8
(0.6) 		115.1
(-2) 		99.5
(-3) 		169.4
(1.6) 		191
(3) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186842_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10456
Sequence Type Exemplar sequence
Representative Public ID Y77E11A.6 NCBI
Target Description Y77E11A.6 /REP_DB=WormBase Gene ID /WP=CE25620 /TR=Q9N4B7 /GB=AAF36076.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067675(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067675 NCBI Caenorhabditis elegans Y77E11A.6 (Y77E11A.6) mRNA, complete cds. 11/11 None
SNAP00000027302 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1407577:1410036:-1 11/11 None
Y77E11A.6 ENSEMBL cdna:known chromosome:CEL140:IV:1408617:1410036:-1 gene:Y77E11A.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1408711-1410042(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y77E11A.6
Entrez Gene 176950 Entrez gene
SwissProt Q9N4B7 EMBL-EBI
WormBase CE25620 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1585634_S_AT similar to DNA-directed RNA polymerases III 12.5 kDa polypeptide (RNA polymerase III C11 subunit) (HsC11p) (hRPC11) cfa
CANINE_2:CFA.10155.1.A1_AT similar to DNA-directed RNA polymerases III 12.5 kDa polypeptide (RNA polymerase III C11 subunit) (HsC11p) (hRPC11) cfa
CANINE_2:CFAAFFX.30157.1.S1_S_AT similar to DNA-directed RNA polymerases III 12.5 kDa polypeptide (RNA polymerase III C11 subunit) (HsC11p) (hRPC11) cfa
HG-U133B:222766_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HG-U133_PLUS_2:222766_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HG-U133_PLUS_2:218866_S_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HG-U133A:218866_S_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HG-FOCUS:218866_S_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HG-U133A_2:218866_S_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HU35KSUBC:RC_AA126951_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
U133_X3P:G7706498_3P_A_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
HG-U95B:56954_AT polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa hs
MOE430A:1439266_A_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430A_2:1439266_A_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430_2:1439266_A_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MG-U74AV2:104304_R_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MG-U74AV2:104303_I_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MG-U74BV2:108360_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MG-U74BV2:162966_I_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOE430A:1422753_A_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430A_2:1422753_A_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430_2:1422753_A_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOE430A:1422752_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430A_2:1422752_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430_2:1422752_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOE430A:1450737_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430A_2:1450737_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MOUSE430_2:1450737_AT polymerase (RNA) III (DNA directed) polypeptide K mm
MG-U74BV2:105310_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOE430B:1440735_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOUSE430_2:1440735_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOUSE430_2:1456731_X_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOE430A:1456731_X_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOUSE430A_2:1456731_X_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOUSE430_2:1457732_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MOE430B:1457732_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
MU19KSUBB:TC31022_AT Polymerase (RNA) III (DNA directed) polypeptide K, mRNA (cDNA clone MGC:30983 IMAGE:5253879) mm
RG-U34A:RC_AI105044_AT estrous-specific protein, 250 kDa rn
RG-U34C:RC_AI228582_S_AT estrous-specific protein, 250 kDa rn
RAE230A:1398875_AT estrous-specific protein, 250 kDa rn
RAT230_2:1398875_AT estrous-specific protein, 250 kDa rn
YEAST_2:1779020_AT RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6350 transcription inferred from electronic annotation QuickGO AmiGO
6354 RNA elongation inferred from electronic annotation QuickGO AmiGO
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3899 DNA-directed RNA polymerase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36076 Hypothetical protein Y77E11A.6 [Caenorhabditis elegans] ref|NP_500076.1| Y77E11A.6 [Caenorhabditis elegans] 5.0E-63
blast CAE68266 Hypothetical protein CBG13943 [Caenorhabditis briggsae] 3.0E-54
blast AAF36076 Hypothetical protein Y77E11A.6 [Caenorhabditis elegans] ref|NP_500076.1| Y77E11A.6 [Caenorhabditis elegans] 4.0E-54
blast CAE68266 Hypothetical protein CBG13943 [Caenorhabditis briggsae] 1.0E-46
blast AAH61683 MGC68582 protein [Xenopus laevis] 4.0E-38
blast AAM83406 POLR3K [Sphoeroides nephelus] 2.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR001222 EMBL-EBI Transcription factor TFIIS 4.8E-19
Pfam IPR001222 EMBL-EBI Transcription factor TFIIS 7.4E-14
Sequence
Target Sequence 
>C. ELEGANS:186842_AT
ggaatgcggctgtgttctacaaatcgagtctggcgaacaatgtatgagattctcgtgtcc
cgcgtgtccctacgtgtgtcccgttacacaaactgtcacaagtcgtatatatccaaaact
taaggatattgatgatgtgctcggtggtccaggagcatgggctaatgctcaggttaccga
tgaaacgtgcccggtatgctcccacggccgggcctatttcatgcaacttcagacaagatc
cgccgatgaaccttcgacaatcttctacagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAATGCGGCTGTGTTCTACAAATC 52 533 30 Antisense
ATGTATGAGATTCTCGTGTCCCGCG 57 49 69 Antisense
TGTCCCGTTACACAAACTGTCACAA 197 475 106 Antisense
ACTGTCACAAGTCGTATATATCCAA 175 97 121 Antisense
TATTGATGATGTGCTCGGTGGTCCA 251 665 156 Antisense
CTCGGTGGTCCAGGAGCATGGGCTA 439 231 169 Antisense
ATGGGCTAATGCTCAGGTTACCGAT 438 49 186 Antisense
CCGATGAAACGTGCCCGGTATGCTC 459 261 206 Antisense
GGCCTATTTCATGCAACTTCAGACA 638 543 240 Antisense
ACAAGATCCGCCGATGAACCTTCGA 363 113 262 Antisense
GAACCTTCGACAATCTTCTACAGAT 20 347 277 Antisense
Sequence Source Affymetrix Proprietary Database