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[186836_at]

Description Y52B11A.10 /REP_DB=WormBase Gene ID /WP=CE20298 /TR=Q9U255 /GB=CAB63391.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 38      4grp_high_8_8 > 03      6grp_total > 37      4grp_total > 13      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 403
1 2 3 4 range
A 317.6
(1.3)
P 		145.1
(-2.7)
P 		130.5
(-3)
A 		391.6
(3)
P 		261
B 320.5
(0.1)
P 		115.4
(-3)
A 		188.6
(-1.9)
M 		518.3
(3)
P 		403
C 341.7
(1.1)
P 		250.7
(-1.4)
A 		194.4
(-3)
P 		412.2
(3)
P 		218
avr 326.6
(0.5) 		170.4
(-3) 		171.2
(-3) 		440.7
(3) 		270

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 181.2
(0.4)
A 		229.7
(3)
P 		147.6
(-1.4)
P 		117.2
(-3)
A 		121.5
(-2.8)
P 		188.8
(0.8)
M 		113
B 221.6
(3)
M 		221.1
(3)
P 		121.8
(-3)
P 		164.6
(-0.4)
P 		154.4
(-1)
P 		184.4
(0.8)
P 		100
C 272.4
(3)
P 		137.5
(-2.5)
P 		124.3
(-3)
P 		149.8
(-2)
A 		163.7
(-1.4)
P 		194.3
(-0.2)
P 		148
avr 225.1
(3) 		196.1
(1.1) 		131.2
(-3) 		143.9
(-2.2) 		146.5
(-2) 		189.2
(0.7) 		94
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 115.6
(3)
P 		31.5
(-3)
P 		34.1
(-2.8)
A 		57.3
(-1.2)
P 		84
B 102.2
(2.4)
P 		28.5
(-3)
A 		43.7
(-1.9)
M 		110.4
(3)
P 		82
C 113.1
(3)
P 		72.1
(-2.4)
A 		67.7
(-3)
P 		69.5
(-2.8)
P 		45
avr 110.3
(3) 		44
(-3) 		48.5
(-2.6) 		79.1
(0.2) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 33.4
(-2.6)
A 		81.7
(3)
P 		32.1
(-2.7)
P 		29.7
(-3)
A 		36.7
(-2.2)
P 		46
(-1.1)
M 		52
B 77.1
(3)
M 		73.6
(2.6)
P 		29.6
(-3)
P 		43.6
(-1.2)
P 		62.7
(1.2)
P 		68.8
(2)
P 		48
C 101
(3)
P 		42
(-2)
P 		31.6
(-2.9)
P 		30.1
(-3)
A 		53
(-1.1)
P 		65
(-0)
P 		71
avr 70.5
(3) 		65.8
(2.3) 		31.1
(-3) 		34.5
(-2.5) 		50.8
(-0) 		59.9
(1.4) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186836_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1904
Sequence Type Exemplar sequence
Representative Public ID Y52B11A.10 NCBI
Target Description Y52B11A.10 /REP_DB=WormBase Gene ID /WP=CE20298 /TR=Q9U255 /GB=CAB63391.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060460(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060460 NCBI Caenorhabditis elegans Y52B11A.10 (Y52B11A.10) mRNA, complete cds. 11/11 A 
SNAP00000015594 ENSEMBL cdna:SNAP chromosome:CEL140:I:11038766:11060523:1 11/11 None
GENEFINDER00000015599 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11047187:11060523:1 11/11 None
Y52B11A.10 ENSEMBL cdna:known chromosome:CEL140:I:11047181:11060597:1 gene:Y52B11A.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11047117-11060454(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y52B11A.10
Entrez Gene 173006 Entrez gene
SwissProt Q9U255 EMBL-EBI
WormBase CE20298 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250066_AT MA3 domain-containing protein at
DROSOPHILA_2:1625269_AT dm
DROSGENOME1:148042_AT dm
CHICKEN:GGA.7382.1.S1_AT similar to CG9004-PA gga
HU35KSUBA:RC_AA022632_AT nucleolar protein with MIF4G domain 1 hs
HU35KSUBA:HUM_ALU_AT nucleolar protein with MIF4G domain 1 hs
HU35KSUBB:HUM_ALU_AT nucleolar protein with MIF4G domain 1 hs
HU35KSUBC:HUM_ALU_AT nucleolar protein with MIF4G domain 1 hs
HU35KSUBD:HUM_ALU_AT nucleolar protein with MIF4G domain 1 hs
HUGENEFL:HUM_ALU_AT nucleolar protein with MIF4G domain 1 hs
HC-G110:HUM_ALU_AT nucleolar protein with MIF4G domain 1 hs
HU35KSUBC:RC_H97788_AT nucleolar protein with MIF4G domain 1 hs
U133_X3P:HS.42443.0.A1_3P_AT nucleolar protein with MIF4G domain 1 hs
U133_X3P:HS.15825.0.A1_3P_AT nucleolar protein with MIF4G domain 1 hs
U133_X3P:229220_3P_X_AT nucleolar protein with MIF4G domain 1 hs
HG-U133_PLUS_2:227161_AT nucleolar protein with MIF4G domain 1 hs
HG-U133B:227161_AT nucleolar protein with MIF4G domain 1 hs
HG-U133_PLUS_2:229220_X_AT nucleolar protein with MIF4G domain 1 hs
HG-U133B:229220_X_AT nucleolar protein with MIF4G domain 1 hs
HG-U95B:47911_AT nucleolar protein with MIF4G domain 1 hs
HG-U95B:53540_AT nucleolar protein with MIF4G domain 1 hs
HG-U95E:72456_R_AT nucleolar protein with MIF4G domain 1 hs
HG-U95D:71632_AT Nucleolar protein with MIF4G domain 1 hs
MG-U74CV2:130592_AT B16 F10Y cells cDNA, RIKEN full-length enriched library, clone:G370137F12 product:hypothetical Initiation factor eIF-4 gamma, MA3/ARM repeat fold containing protein, full insert sequence mm
MU19KSUBA:TC25199_AT B16 F10Y cells cDNA, RIKEN full-length enriched library, clone:G370137F12 product:hypothetical Initiation factor eIF-4 gamma, MA3/ARM repeat fold containing protein, full insert sequence mm
YEAST_2:1770607_AT Essential nuclear protein with a possible role in the osmoregulatory glycerol response; interacts with phospholipase C (Plc1p); putative homolog of human NOM1 which is implicated in acute myeloid leukemia Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63391 Hypothetical protein Y52B11A.10 [Caenorhabditis elegans] ref|NP_492861.1| Y52B11A.10 [Caenorhabditis elegans] 0.0
blast CAE71829 Hypothetical protein CBG18870 [Caenorhabditis briggsae] 0.0
blast CAB63390 Hypothetical protein Y52B11A.8 [Caenorhabditis elegans] ref|NP_492859.1| Y52B11A.8 [Caenorhabditis elegans] 2.0E-69
blast XP_685763 PREDICTED: similar to nucleolar protein with MIF4G domain 1 [Danio rerio] 9.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 2.3E-38
Pfam IPR003891 EMBL-EBI Initiation factor eIF-4 gamma, MA3 8.3E-35
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 1.6E-27
Pfam IPR003891 EMBL-EBI Initiation factor eIF-4 gamma, MA3 8.3E-35
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 2.3E-38
Pfam IPR003891 EMBL-EBI Initiation factor eIF-4 gamma, MA3 8.3E-35
Sequence
Target Sequence 
>C. ELEGANS:186836_AT
tcccgccggaaatggtctcactggccacgaaagccggaatgaattcagaagttcgaagac
acattttttgtagtgttgccagcgccgacgacgaggacgacgcgttcgaacgacttttga
aacttcaattaaaaggcgaaaaagaacgagaacttgttcatgtgcttattgctatgatga
tgaaggagaagacgtacaacgcgttttatgccacgttgttgcaacgattctgcgaattta
ataagcgatttgtgatcaccctccaattcgccctatgggatcgtctacgtgaatgtgatc
aattgaaaccattccagagaggatcactggctcaacttcttcagcatttgatttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCGCCGGAAATGGTCTCACTGGC 35 597 1844 Antisense
GTCTCACTGGCCACGAAAGCCGGAA 274 457 1858 Antisense
CATTTTTTGTAGTGTTGCCAGCGCC 586 213 1905 Antisense
GAGGACGACGCGTTCGAACGACTTT 179 403 1936 Antisense
GAACTTGTTCATGTGCTTATTGCTA 122 351 1993 Antisense
GACGTACAACGCGTTTTATGCCACG 123 369 2034 Antisense
GCCACGTTGTTGCAACGATTCTGCG 182 279 2053 Antisense
TAAGCGATTTGTGATCACCCTCCAA 99 639 2085 Antisense
ATTCGCCCTATGGGATCGTCTACGT 453 7 2109 Antisense
AGAGGATCACTGGCTCAACTTCTTC 279 69 2161 Antisense
CAACTTCTTCAGCATTTGATTTCCA 421 189 2176 Antisense
Sequence Source Affymetrix Proprietary Database