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[186819_s_at]

Description Y45G12C.3 /REP_DB=WormBase Gene ID /WP=CE20883 /TR=Q9N2L0 /GB=AAF60578.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 43      4grp_total > 42      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 11.2
(-2.3)
A 		45.2
(3)
A 		11.5
(-2.2)
A 		6.5
(-3)
A 		39
B 19.7
(-3)
A 		33.3
(1.9)
A 		36.5
(3)
A 		21.3
(-2.4)
A 		17
C 7.3
(-3)
A 		42.6
(3)
A 		17.7
(-1.2)
A 		38.9
(2.4)
A 		35
avr 12.7
(-3) 		40.4
(3) 		21.9
(-1) 		22.2
(-0.9) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 53.7
(1.5)
A 		54.8
(1.6)
A 		70.7
(3)
A 		39.5
(0.3)
A 		14.6
(-1.9)
A 		1.7
(-3)
A 		69
B 6.5
(-2.7)
A 		45.4
(3)
A 		43.6
(2.7)
A 		4.3
(-3)
A 		16.5
(-1.2)
A 		6.7
(-2.6)
A 		41
C 13.9
(-2.4)
A 		26.9
(-1.2)
A 		73.4
(3)
A 		7.7
(-3)
A 		22.6
(-1.6)
A 		22.6
(-1.6)
A 		66
avr 24.7
(-1.3) 		42.4
(0.7) 		62.6
(3) 		17.2
(-2.2) 		17.9
(-2.1) 		10.3
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 4.1
(-0.9)
A 		9.8
(3)
A 		3
(-1.6)
A 		1
(-3)
A 		9
B 6.3
(-0.3)
A 		8.2
(2.6)
A 		8.5
(3)
A 		4.5
(-3)
A 		4
C 2.4
(-3)
A 		12.3
(3)
A 		6.2
(-0.7)
A 		6.6
(-0.5)
A 		10
avr 4.3
(-2.7) 		10.1
(3) 		5.9
(-1.1) 		4
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 9.9
(-0)
A 		19.5
(3)
A 		15.4
(1.7)
A 		10
(0)
A 		4.4
(-1.7)
A 		0.4
(-3)
A 		19
B 2.3
(-2.5)
A 		15.1
(3)
A 		10.6
(1.1)
A 		1.1
(-3)
A 		6.7
(-0.6)
A 		2.5
(-2.4)
A 		14
C 5.2
(-1.7)
A 		8.2
(-0.7)
A 		18.7
(3)
A 		1.5
(-3)
A 		7.3
(-1)
A 		7.6
(-0.9)
A 		17
avr 5.8
(-1.8) 		14.3
(2.7) 		14.9
(3) 		4.2
(-2.6) 		6.1
(-1.6) 		3.5
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186819_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15450
Sequence Type Exemplar sequence
Representative Public ID Y45G12C.3 NCBI
Target Description Y45G12C.3 /REP_DB=WormBase Gene ID /WP=CE20883 /TR=Q9N2L0 /GB=AAF60578.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071270(11), NM_071297(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071270 NCBI Caenorhabditis elegans F56A4.4 (F56A4.4) mRNA, complete cds. 11/11 None
NM_071297 NCBI Caenorhabditis elegans Y45G12C.3 (Y45G12C.3) mRNA, complete cds. 10/11 None
SNAP00000032941 ENSEMBL cdna:SNAP chromosome:CEL140:V:2443941:2449071:1 9/11 None
GENEFINDER00000032953 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2447723:2449071:1 11/11 None
SNAP00000043314 ENSEMBL cdna:SNAP chromosome:CEL140:V:2552220:2557350:1 9/11 None
GENEFINDER00000043331 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2556002:2557350:1 11/11 None
F56A4.4 ENSEMBL cdna:known chromosome:CEL140:V:2447723:2449071:1 gene:F56A4.4 11/11 None
Y45G12C.3 ENSEMBL cdna:known chromosome:CEL140:V:2556002:2557350:1 gene:Y45G12C.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2447722-2449071(+) 85.47 100.0
chrV:2556001-2557350(+) 85.47 100.0
Public Domain and Genome References
Gene Title

Ensembl F56A4.4
Y45G12C.3
Entrez Gene 186347 Entrez gene
189932 Entrez gene
SwissProt Q9N2L0 EMBL-EBI
WormBase CE32258 Wormbase
CE32258 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503671 F56A4.4 [Caenorhabditis elegans] 1.0E-97
blast AAF39842 Hypothetical protein F56A4.4 [Caenorhabditis elegans] ref|NP_503698.2| Y45G12C.3 [Caenorhabditis elegans] gb|AAF60578.2| Hypothetical protein Y45G12C.3 [Caenorhabditis elegans] 1.0E-82
blast NP_503671 F56A4.4 [Caenorhabditis elegans] 2.0E-78
blast AAF39842 Hypothetical protein F56A4.4 [Caenorhabditis elegans] ref|NP_503698.2| Y45G12C.3 [Caenorhabditis elegans] gb|AAF60578.2| Hypothetical protein Y45G12C.3 [Caenorhabditis elegans] 4.0E-78
Protein Domains
Database ID Description E-Value
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 7.8E-14
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.1E-9
Sequence
Target Sequence 
>C. ELEGANS:186819_S_AT
aactttactacttcaatcttcgaggattcggagaatacatccgtcttcttttcctcgaca
atggcatccaattcgaagacattcgatttgattacgaggggaaggaatggcaagagttca
aaaaaggaatgcttctcgggcagttgccatgtttgaaggtggacaggcaggaaattgtgc
agaccggagcaattatgcgtcatttgggaagagttcatgaaaaaaatttggcgagaaatt
tcaatttttcgaaaaaatatttaatgggaaaactaacatttctcgaaaaatgtggcctga
acggatccaacgagcaagaggcgaccttcctcgacatgtttttcgaaggagtccgcgatg
ttcgttggaaatacgtccgctcttcgaggactctgaagtcgttctgggagagaatgtgga
agagaccaaatttgaacgcgtacctaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTTTACTACTTCAATCTTCGAGG 652 137 26 Antisense
GTCTTCTTTTCCTCGACAATGGCAT 196 469 68 Antisense
TTCCTCGACAATGGCATCCAATTCG 459 699 76 Antisense
AAGGAATGCTTCTCGGGCAGTTGCC 317 161 149 Antisense
CGGGCAGTTGCCATGTTTGAAGGTG 570 241 162 Antisense
AGGAAATTGTGCAGACCGGAGCAAT 61 55 194 Antisense
AAATGTGGCCTGAACGGATCCAACG 553 119 313 Antisense
GTTTTTCGAAGGAGTCCGCGATGTT 104 447 363 Antisense
GTTCGTTGGAAATACGTCCGCTCTT 222 441 385 Antisense
GTCCGCTCTTCGAGGACTCTGAAGT 66 475 400 Antisense
GACCAAATTTGAACGCGTACCTAGA 3 383 449 Antisense
Sequence Source Affymetrix Proprietary Database