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[186816_at]

Description Y76B12C.3 /REP_DB=WormBase Gene ID /WP=CE28149 /TR=Q9N4C6 /GB=AAF36064.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 30      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 52      6grp_total > 53      4grp_total > 58      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 583
1 2 3 4 range
A 537.7
(-3)
P 		1026.3
(3)
P 		691.1
(-1.1)
P 		567.5
(-2.6)
P 		489
B 701.8
(-0.8)
P 		727.3
(-0.4)
P 		913
(3)
P 		583.6
(-3)
P 		329
C 676.9
(0.4)
P 		854.4
(3)
P 		759.5
(1.6)
P 		443.3
(-3)
P 		411
avr 638.8
(-1.1) 		869.3
(3) 		787.9
(1.6) 		531.5
(-3) 		338

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 566
1 2 3 4 5 6 range
A 598.3
(-1.3)
P 		680
(-0.5)
P 		1005.7
(3)
P 		691.7
(-0.3)
P 		440
(-3)
P 		595.7
(-1.3)
P 		566
B 585.5
(-3)
P 		705.2
(-0.9)
P 		929
(3)
P 		758.5
(0)
P 		617.3
(-2.4)
P 		643.2
(-2)
P 		344
C 755.4
(3)
P 		667.7
(0.7)
P 		735
(2.5)
P 		531.7
(-2.9)
P 		638
(-0.1)
P 		527
(-3)
P 		228
avr 646.4
(-1.5) 		684.3
(-0.8) 		889.9
(3) 		660.6
(-1.2) 		565.1
(-3) 		588.6
(-2.6) 		325
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 195.8
(1.8)
P 		222.8
(3)
P 		180.7
(1.2)
P 		83.1
(-3)
P 		140
B 223.8
(3)
P 		179.6
(0.3)
P 		211.6
(2.3)
P 		124.4
(-3)
P 		99
C 224.1
(1.7)
P 		245.6
(2.4)
P 		264.7
(3)
P 		74.7
(-3)
P 		190
avr 214.6
(2.8) 		216
(2.9) 		219
(3) 		94.1
(-3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 110.3
(-3)
P 		241.8
(3)
P 		219
(2)
P 		175.4
(-0)
P 		132.8
(-2)
P 		145
(-1.4)
P 		132
B 203.7
(-2.7)
P 		234.7
(1.1)
P 		225.6
(-0)
P 		200.9
(-3)
P 		250.8
(3)
P 		239.8
(1.7)
P 		50
C 280
(3)
P 		203.7
(0.4)
P 		187.1
(-0.2)
P 		106.9
(-3)
P 		206.5
(0.5)
P 		176.4
(-0.6)
P 		173
avr 198
(0.4) 		226.7
(3) 		210.6
(1.5) 		161.1
(-3) 		196.7
(0.3) 		187.1
(-0.6) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186816_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10961
Sequence Type Exemplar sequence
Representative Public ID Y76B12C.3 NCBI
Target Description Y76B12C.3 /REP_DB=WormBase Gene ID /WP=CE28149 /TR=Q9N4C6 /GB=AAF36064.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067751(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067751 NCBI Caenorhabditis elegans Y76B12C.3 (Y76B12C.3) mRNA, complete cds. 9/11 A 
SNAP00000007750 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1971725:1975539:1 9/11 None
GENEFINDER00000007757 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1973707:1974723:1 9/11 None
Y76B12C.3 ENSEMBL cdna:known chromosome:CEL140:IV:1973705:1975609:1 gene:Y76B12C.3 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1973712-1975545(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y76B12C.3
Entrez Gene 176999 Entrez gene
SwissProt Q9N4C6 EMBL-EBI
WormBase CE28149 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.20706.1.S1_AT similar to 15 kDa selenoprotein isoform 1 precursor cfa
CANINE_2:CFAAFFX.30993.1.S1_S_AT similar to 15 kDa selenoprotein isoform 1 precursor cfa
CANINE_2:CFAAFFX.30994.1.S1_S_AT similar to 15 kDa selenoprotein isoform 1 precursor cfa
DROSOPHILA_2:1628883_AT dm
DROSGENOME1:148966_AT dm
HG-U133_PLUS_2:200902_AT 15 kDa selenoprotein hs
HG-U133A:200902_AT 15 kDa selenoprotein hs
HG-U133A_2:200902_AT 15 kDa selenoprotein hs
HG-FOCUS:200902_AT 15 kDa selenoprotein hs
HU35KSUBA:AA092376_AT 15 kDa selenoprotein hs
HU35KSUBC:RC_AA001402_AT 15 kDa selenoprotein hs
U133_X3P:G4759095_3P_AT 15 kDa selenoprotein hs
HG-U95AV2:38820_AT 15 kDa selenoprotein hs
HG-U95E:78899_AT 15 kDa selenoprotein hs
HU35KSUBC:RC_W90605_AT 15 kDa selenoprotein hs
MU11KSUBA:AA241085_S_AT selenoprotein mm
MG-U74AV2:96249_AT selenoprotein mm
MG-U74AV2:160360_AT selenoprotein mm
MOE430A:1448903_AT selenoprotein mm
MOUSE430A_2:1448903_AT selenoprotein mm
MOUSE430_2:1448903_AT selenoprotein mm
MOE430B:1454525_AT selenoprotein mm
MOUSE430_2:1454525_AT selenoprotein mm
MU19KSUBC:TC39133_AT Selenoprotein, mRNA (cDNA clone MGC:30351 IMAGE:5003585) mm
RG-U34B:RC_AA945698_AT selenoprotein rn
RAE230A:1398752_AT selenoprotein rn
RAT230_2:1398752_AT selenoprotein rn
Protein Similarities
Method ID Description E-Value
blast AAF36064 Hypothetical protein Y76B12C.3 [Caenorhabditis elegans] ref|NP_500152.1| Y76B12C.3 [Caenorhabditis elegans] 2.0E-77
blast AAF36064 Hypothetical protein Y76B12C.3 [Caenorhabditis elegans] ref|NP_500152.1| Y76B12C.3 [Caenorhabditis elegans] 5.0E-77
blast CAE74693 Hypothetical protein CBG22507 [Caenorhabditis briggsae] 2.0E-67
blast CAE74693 Hypothetical protein CBG22507 [Caenorhabditis briggsae] 4.0E-67
blast AAF36064 Hypothetical protein Y76B12C.3 [Caenorhabditis elegans] ref|NP_500152.1| Y76B12C.3 [Caenorhabditis elegans] 2.0E-50
blast CAE74693 Hypothetical protein CBG22507 [Caenorhabditis briggsae] 5.0E-42
blast XP_394140 PREDICTED: similar to ENSANGP00000011457 [Apis mellifera] 2.0E-20
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000007750 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:186816_AT
ggtttcgcctatgttcggtgaagttgaagagtataaaattgatgtagaggagtgcaaggc
ggcgggattcaatccggagactttaaagtgcggattatgcgaacgactatcggattatca
tctggaaacgctgctcaccgattgtcttcagtgttgtattaaggaagaagagtttaagca
cgaaaaatatccaacggcaattctggaagtctgcgagtgcaatttggctcgattcccaca
agttcaagtgaaattttcaacacccaaaaaatcaataattccagcattcgtccata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTTCGCCTATGTTCGGTGAAGTT 176 503 45 Antisense
GAGGAGTGCAAGGCGGCGGGATTCA 56 405 91 Antisense
GGCGGCGGGATTCAATCCGGAGACT 19 537 102 Antisense
AAGTGCGGATTATGCGAACGACTAT 227 159 130 Antisense
GAACGACTATCGGATTATCATCTGG 334 347 145 Antisense
TTATCATCTGGAAACGCTGCTCACC 645 677 159 Antisense
TGCTCACCGATTGTCTTCAGTGTTG 151 581 176 Antisense
ACCGATTGTCTTCAGTGTTGTATTA 210 89 181 Antisense
CAACGGCAATTCTGGAAGTCTGCGA 320 187 236 Antisense
GTTCAAGTGAAATTTTCAACACCCA 187 445 286 Antisense
TCAATAATTCCAGCATTCGTCCATA 668 633 316 Antisense
Sequence Source Affymetrix Proprietary Database