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[186813_at]

Description ZC21.6 /REP_DB=WormBase Gene ID /WP=CE00348 /TR=SW:P34590 /GB=AAA28166.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 45      6grp_total > 46      4grp_total > 04      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 66.5
(-2.7)
P 		60.9
(-3)
A 		91.4
(-1.5)
P 		180.1
(3)
P 		119
B 83.4
(-0.6)
A 		69.8
(-1.2)
A 		26.7
(-3)
A 		170.3
(3)
P 		144
C 95.3
(-1.1)
P 		47.6
(-3)
M 		57.9
(-2.6)
P 		194.5
(3)
P 		147
avr 81.7
(-1.9) 		59.4
(-3) 		58.7
(-3) 		181.6
(3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 76.8
(-0.4)
P 		59.4
(-2.3)
A 		53.1
(-3)
A 		82.7
(0.3)
P 		65.8
(-1.6)
M 		107.2
(3)
P 		54
B 68.1
(0.6)
P 		85.5
(3)
P 		72.7
(1.2)
P 		80.8
(2.4)
P 		41.9
(-3)
P 		74.2
(1.4)
P 		44
C 39.3
(-2.9)
M 		109.5
(3)
A 		60
(-1.2)
M 		98.6
(2.1)
P 		38.7
(-3)
A 		76.9
(0.2)
P 		71
avr 61.4
(-1) 		84.8
(2.6) 		61.9
(-1) 		87.4
(3) 		48.8
(-3) 		86.1
(2.8) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 24.2
(2)
P 		13.2
(-3)
A 		23.9
(1.9)
P 		26.4
(3)
P 		13
B 26.6
(1.1)
A 		17.2
(-0.8)
A 		6.2
(-3)
A 		36.3
(3)
P 		30
C 31.5
(2.6)
P 		13.7
(-3)
M 		20.2
(-1)
P 		32.8
(3)
P 		19
avr 27.4
(1.5) 		14.7
(-3) 		16.8
(-2.3) 		31.8
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 14.2
(-1.9)
P 		21.1
(0.9)
A 		11.6
(-3)
A 		21
(0.9)
P 		19.9
(0.4)
M 		26.1
(3)
P 		15
B 23.7
(0.5)
P 		28.5
(3)
P 		17.6
(-2.7)
P 		21.4
(-0.7)
P 		17
(-3)
P 		27.7
(2.6)
P 		12
C 14.6
(-2.4)
M 		33.4
(3)
A 		15.3
(-2.2)
M 		19.8
(-0.9)
P 		12.5
(-3)
A 		25.7
(0.8)
P 		21
avr 17.5
(-1.7) 		27.7
(3) 		14.8
(-3) 		20.7
(-0.3) 		16.5
(-2.2) 		26.5
(2.4) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186813_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8003
Sequence Type Exemplar sequence
Representative Public ID ZC21.6 NCBI
Target Description ZC21.6 /REP_DB=WormBase Gene ID /WP=CE00348 /TR=SW:P34590 /GB=AAA28166.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066481(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066481 NCBI Caenorhabditis elegans ZC21.6a (ZC21.6) mRNA, complete cds. 11/11 None
GENEFINDER00000000310 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8542889:8545009:-1 10/11 None
SNAP00000000307 ENSEMBL cdna:SNAP chromosome:CEL140:III:8543454:8545495:-1 11/11 None
ZC21.6a ENSEMBL cdna:known chromosome:CEL140:III:8543454:8544866:-1 gene:ZC21.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_181952 8/11 Cross Hyb Matching Probes None
ZC21.6b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
191054 2 1 NM_066481 186813_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8543448-8544861(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC21.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO12393 Hypothetical protein ZC21.6a [Caenorhabditis elegans] ref|NP_498882.2| ZC21.6a [Caenorhabditis elegans] 1.0E-160
blast AAO12394 Hypothetical protein ZC21.6b [Caenorhabditis elegans] ref|NP_871681.1| ZC21.6b [Caenorhabditis elegans] sp|P34590|YN56_CAEEL Hypothetical protein ZC21.6 in chromosome III 1.0E-153
blast AAO12393 Hypothetical protein ZC21.6a [Caenorhabditis elegans] ref|NP_498882.2| ZC21.6a [Caenorhabditis elegans] 1.0E-145
blast AAO12394 Hypothetical protein ZC21.6b [Caenorhabditis elegans] ref|NP_871681.1| ZC21.6b [Caenorhabditis elegans] sp|P34590|YN56_CAEEL Hypothetical protein ZC21.6 in chromosome III 1.0E-138
blast CAE71234 Hypothetical protein CBG18104 [Caenorhabditis briggsae] 1.0E-126
blast AAO12394 Hypothetical protein ZC21.6b [Caenorhabditis elegans] ref|NP_871681.1| ZC21.6b [Caenorhabditis elegans] sp|P34590|YN56_CAEEL Hypothetical protein ZC21.6 in chromosome III 1.0E-125
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498882.2 5 20-42,63-85,134-156,176-198,213-232
ZC21.6a 5 20-42,63-85,134-156,176-198,213-232
GENEFINDER00000000310 6 23-45,68-90,111-133,182-204,224-246,261-280
SNAP00000000307 7 35-57,70-89,113-135,156-178,227-249,269-291,306-325
Sequence
Target Sequence 
>C. ELEGANS:186813_AT
cataaaatctcatccgggttcgcggagcctgattgtcacaattcttccaccaattgtctt
ttattgcatctctgttgtcagttatgtattcattattcatcttcttttctcaagacgaaa
agtatcctcatatcatcgacattggggttccatgcttcgcctcggtattcatttgatttt
atttgctggaacatgtgcattgactggaactgcctatcttggatcattttctattggaga
catttgtgagaattataaaacggttagtttggaaagtttcaattattctgactataaatt
caatggagaagattgtagatttttaatattttcagttttgaaaacaaagcaaaagttgtt
agaaaagagcaagaactatttgaggaatgaaccaatgtgtcttgacccagttgtactgta
tatgtggaatacagcagctgcccttatcggatggatatgtcgaatggttattgatgcaac
agttgatacggtagacattgcgtgtttcttcgtgtttcacacaaaggtattcatcgatat
gtgttcatcggtttgtaatttcagtttaatgacgacgtgcttcggcgaacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATAAAATCTCATCCGGGTTCGCGG 239 211 372 Antisense
GATTGTCACAATTCTTCCACCAATT 242 435 402 Antisense
GTCTTTTATTGCATCTCTGTTGTCA 324 469 427 Antisense
ATTCATTATTCATCTTCTTTTCTCA 635 11 459 Antisense
TCGACATTGGGGTTCCATGCTTCGC 208 601 507 Antisense
CTTCGCCTCGGTATTCATTTGATTT 24 221 526 Antisense
GACTGGAACTGCCTATCTTGGATCA 133 377 573 Antisense
GAACCAATGTGTCTTGACCCAGTTG 490 345 760 Antisense
GAATACAGCAGCTGCCCTTATCGGA 276 327 798 Antisense
GACATTGCGTGTTTCTTCGTGTTTC 392 371 865 Antisense
TAATGACGACGTGCTTCGGCGAACG 468 633 938 Antisense
Sequence Source Affymetrix Proprietary Database