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[186812_s_at]

Description Y32H12A.8 /REP_DB=WormBase Gene ID /WP=CE28516 /TR=Q9N533 /GB=AAF60491.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 05      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 166.8
(2.9)
P 		105.7
(-1.1)
A 		76.8
(-3)
A 		168.9
(3)
P 		92
B 111.5
(0.9)
P 		29.4
(-3)
A 		60.8
(-1.5)
A 		155
(3)
P 		126
C 123.7
(-1.9)
P 		91.8
(-3)
A 		96.9
(-2.8)
P 		265.3
(3)
P 		174
avr 134
(-0.1) 		75.6
(-3) 		78.2
(-2.9) 		196.4
(3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 139.4
(3)
P 		137.7
(2.9)
P 		122.7
(2.1)
P 		113.9
(1.6)
P 		31.8
(-3)
A 		96.3
(0.6)
P 		108
B 115.1
(-0.4)
P 		106.9
(-0.9)
P 		167.5
(3)
P 		121
(0)
P 		73.7
(-3)
A 		120.9
(0)
P 		94
C 169.8
(3)
P 		108.5
(-0.4)
P 		95.8
(-1.1)
P 		75.1
(-2.3)
P 		62.5
(-3)
A 		76
(-2.2)
P 		107
avr 141.4
(3) 		117.7
(1.3) 		128.7
(2.1) 		103.3
(0.3) 		56
(-3) 		97.7
(-0.1) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 60.7
(3)
P 		22.9
(-2.6)
A 		20.1
(-3)
A 		24.7
(-2.3)
P 		41
B 35.6
(3)
P 		7.3
(-3)
A 		14.1
(-1.6)
A 		33
(2.4)
P 		28
C 40.9
(1.8)
P 		26.4
(-3)
A 		33.8
(-0.6)
P 		44.7
(3)
P 		18
avr 45.7
(3) 		18.9
(-3) 		22.7
(-2.1) 		34.1
(0.4) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 25.7
(-0.5)
P 		49
(3)
P 		26.7
(-0.4)
P 		28.9
(-0.1)
P 		9.6
(-3)
A 		23.4
(-0.9)
P 		39
B 40
(1)
P 		35.6
(-0.8)
P 		40.7
(1.3)
P 		32.1
(-2.2)
P 		30
(-3)
A 		45.1
(3)
P 		15
C 62.9
(3)
P 		33.1
(-0.7)
P 		24.4
(-1.8)
P 		15.1
(-3)
P 		20.2
(-2.4)
A 		25.5
(-1.7)
P 		48
avr 42.9
(3) 		39.2
(2) 		30.6
(-0.2) 		25.4
(-1.6) 		19.9
(-3) 		31.3
(-0) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186812_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6141
Sequence Type Exemplar sequence
Representative Public ID Y32H12A.8 NCBI
Target Description Y32H12A.8 /REP_DB=WormBase Gene ID /WP=CE28516 /TR=Q9N533 /GB=AAF60491.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065750(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065750 NCBI Caenorhabditis elegans Y32H12A.8 (Y32H12A.8) mRNA, complete cds. 11/11 A 
Y32H12A.8 ENSEMBL cdna:known chromosome:CEL140:III:5385482:5404941:-1 gene:Y32H12A.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5385792-5404937(-) 98.33 100.0
Public Domain and Genome References
Gene Title
Ensembl Y32H12A.8
Entrez Gene 175742 Entrez gene
SwissProt Q9N533 EMBL-EBI
WormBase CE28516 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18851.1.S1_S_AT similar to WD repeat domain 24 cfa
CANINE_2:CFAAFFX.30004.1.S1_S_AT similar to WD repeat domain 24 cfa
DROSOPHILA_2:1640643_A_AT dm
DROSGENOME1:154050_AT dm
CHICKEN:GGAAFFX.11378.1.S1_AT WD repeat domain 24 gga
CHICKEN:GGAAFFX.1637.1.S1_S_AT WD repeat domain 24 gga
HG-U133B:223293_AT WD repeat domain 24 hs
HG-U133_PLUS_2:223293_AT WD repeat domain 24 hs
HU35KSUBA:RC_AA403312_S_AT WD repeat domain 24 hs
HU35KSUBD:RC_AA302632_AT WD repeat domain 24 hs
U133_X3P:G12053230_3P_AT WD repeat domain 24 hs
HG-U95B:48491_AT WD repeat domain 24 hs
HG-U95E:68512_AT WD repeat domain 24 hs
HG-U95D:72188_AT WD repeat domain 24 hs
MOE430B:1455212_AT WD repeat domain 24 mm
MOUSE430_2:1455212_AT WD repeat domain 24 mm
MG-U74CV2:165771_R_AT WD repeat domain 24, mRNA (cDNA clone MGC:47001 IMAGE:5148210) mm
RG-U34C:RC_AI073006_R_AT similar to Hypothetical protein MGC47001 (predicted) rn
RG-U34C:RC_AI179411_AT similar to Hypothetical protein MGC47001 (predicted) rn
RG-U34C:RC_AI073006_S_AT similar to Hypothetical protein MGC47001 (predicted) rn
RAE230A:1373938_AT similar to Hypothetical protein MGC47001 (predicted) rn
RAT230_2:1373938_AT similar to Hypothetical protein MGC47001 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60491 Hypothetical protein Y32H12A.8 [Caenorhabditis elegans] ref|NP_498151.1| Y32H12A.8 [Caenorhabditis elegans] 0.0
blast CAE61332 Hypothetical protein CBG05170 [Caenorhabditis briggsae] 0.0
blast XP_397394 PREDICTED: similar to ENSANGP00000004131 [Apis mellifera] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.01
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.6E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.2
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.22
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0048
Sequence
Target Sequence 
>C. ELEGANS:186812_S_AT
gacgacagacaccgaaagatccaaaatcaatgtctagattcctgaatatgcataattcgg
gttctgattcggattctggttcagaagttcgatcggattcatcttcgtctcttggtggat
caactgagtctttgaacagctcgaaaaaaggaaaacgagctcgccgagcaatgaacaagg
cacaaagagagctgatgcttttaaaactccgatcgaaacctgtaaatccagaagaaacat
ttgctctggaaaaacgagcaattggagcaaaaaagtggaaggttcgacgaaaaatcaacg
agatgttggaaggaattgcggatcttgacaaatttaagattgaggcgaagcttccggcaa
aaaagaaagatttattgaaacatatcaatgaattaattgttgaaaattctgatacagcac
actggtcatggggtgatttgaaaatgcagacgagaatacgaaagattccaccagcaaata
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGACAGACACCGAAAGATCCAAA 280 379 11081 Antisense
GCATAATTCGGGTTCTGATTCGGAT 323 309 11130 Antisense
GTTCGATCGGATTCATCTTCGTCTC 202 443 11167 Antisense
CTTCGTCTCTTGGTGGATCAACTGA 172 221 11183 Antisense
TCAACTGAGTCTTTGAACAGCTCGA 150 631 11200 Antisense
GCTGATGCTTTTAAAACTCCGATCG 574 295 11271 Antisense
ACTCCGATCGAAACCTGTAAATCCA 180 97 11286 Antisense
GGAAGGAATTGCGGATCTTGACAAA 187 533 11388 Antisense
AGATTGAGGCGAAGCTTCCGGCAAA 263 65 11417 Antisense
GATACAGCACACTGGTCATGGGGTG 420 423 11491 Antisense
TACGAAAGATTCCACCAGCAAATAG 321 649 11537 Antisense
Sequence Source Affymetrix Proprietary Database