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[186811_s_at]

Description ZK550.6 /REP_DB=WormBase Gene ID /WP=CE20432 /TR=SW:O62515 /GB=CAB02322.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 49      6grp_total > 75      4grp_total > 16      6grp_highest_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7212
1 2 3 4 range
A 3323.4
(-3)
P 		5256.9
(-1.4)
P 		4909.6
(-1.7)
P 		10482.9
(3)
P 		7160
B 3368.5
(-3)
P 		4661.6
(-1.5)
P 		5774.9
(-0.2)
P 		8572.2
(3)
P 		5204
C 3271.1
(-3)
P 		3869.9
(-2.5)
P 		5303
(-1.2)
P 		9894
(3)
P 		6623
avr 3321
(-3) 		4596.1
(-1.8) 		5329.2
(-1.1) 		9649.7
(3) 		6329

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4852
1 2 3 4 5 6 range
A 4145
(-2.5)
P 		3706.5
(-3)
P 		6061.9
(-0.1)
P 		6893.9
(0.9)
P 		3938.2
(-2.7)
P 		8558.9
(3)
P 		4852
B 4062.1
(-3)
P 		4102.4
(-2.9)
P 		5055.7
(-1.5)
P 		5350.5
(-1)
P 		4978.8
(-1.6)
P 		7967.5
(3)
P 		3905
C 3933.2
(-3)
P 		3991
(-2.9)
P 		4828.3
(-1.6)
P 		6137.3
(0.5)
P 		4369.3
(-2.3)
P 		7672.7
(3)
P 		3740
avr 4046.8
(-2.8) 		3933.3
(-3) 		5315.3
(-1) 		6127.2
(0.2) 		4428.8
(-2.3) 		8066.4
(3) 		4133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 774
1 2 3 4 range
A 1210.1
(-1.9)
P 		1141.1
(-3)
P 		1283.5
(-0.8)
P 		1534.5
(3)
P 		393
B 1074.2
(-3)
P 		1151.3
(-2.4)
P 		1338.2
(-0.9)
P 		1826.7
(3)
P 		753
C 1082.8
(-3)
P 		1112.6
(-2.8)
P 		1848.3
(3)
P 		1668.3
(1.6)
P 		766
avr 1122.4
(-3) 		1135
(-2.9) 		1490
(1) 		1676.5
(3) 		554

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2207
1 2 3 4 5 6 range
A 764.1
(-3)
P 		1318.2
(-0.5)
P 		1320.2
(-0.5)
P 		1748.5
(1.5)
P 		1188.4
(-1.1)
P 		2083.8
(3)
P 		1320
B 1413.4
(-2.4)
P 		1365.5
(-2.5)
P 		1227.7
(-3)
P 		1417.2
(-2.3)
P 		2022.8
(-0.3)
P 		2971
(3)
P 		1743
C 1457.9
(-1.9)
P 		1217.8
(-3)
P 		1228.9
(-3)
P 		1234.2
(-2.9)
P 		1413.8
(-2.1)
P 		2568.5
(3)
P 		1351
avr 1211.8
(-3) 		1300.5
(-2.6) 		1258.9
(-2.8) 		1466.6
(-1.8) 		1541.7
(-1.5) 		2541.1
(3) 		1329
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186811_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10237
Sequence Type Exemplar sequence
Representative Public ID ZK550.6 NCBI
Target Description ZK550.6 /REP_DB=WormBase Gene ID /WP=CE20432 /TR=SW:O62515 /GB=CAB02322.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070661(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070661 NCBI Caenorhabditis elegans ZK550.6 (ZK550.6) mRNA, complete cds. 11/11 None
ZK550.6.1 ENSEMBL cdna:known chromosome:CEL140:IV:17260269:17262440:-1 gene:ZK550.6 11/11 None
ZK550.6.2 ENSEMBL cdna:known chromosome:CEL140:IV:17260295:17262442:-1 gene:ZK550.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17260349-17262437(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK550.6
Entrez Gene 178503 Entrez gene
SwissProt O62515 EMBL-EBI
WormBase CE20432 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1583288_AT similar to phytanoyl-CoA hydroxylase precursor cfa
CANINE_2:CFA.15689.1.A1_AT similar to phytanoyl-CoA hydroxylase precursor cfa
CANINE_2:CFAAFFX.8051.1.S1_AT similar to phytanoyl-CoA hydroxylase precursor cfa
CHICKEN:GGA.13495.1.S1_AT phytanoyl-CoA hydroxylase (Refsum disease) gga
CHICKEN:GGAAFFX.24454.1.S1_AT phytanoyl-CoA hydroxylase (Refsum disease) gga
HG-U133A:203335_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HG-U133_PLUS_2:203335_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HG-U133A_2:203335_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HG-FOCUS:203335_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HU35KSUBA:T83397_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HU35KSUBB:RC_N63845_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
U133_X3P:G5453883_3P_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HG-U95AV2:32724_AT phytanoyl-CoA hydroxylase (Refsum disease) hs
HU35KSUBB:RC_H52835_AT Phytanoyl-CoA hydroxylase (Refsum disease) hs
MU11KSUBA:AF023463_S_AT phytanoyl-CoA hydroxylase mm
MG-U74AV2:96608_AT phytanoyl-CoA hydroxylase mm
MOE430A:1460194_AT phytanoyl-CoA hydroxylase mm
MOUSE430A_2:1460194_AT phytanoyl-CoA hydroxylase mm
MOUSE430_2:1460194_AT phytanoyl-CoA hydroxylase mm
MG-U74BV2:116805_AT Phytanoyl-CoA hydroxylase, mRNA (cDNA clone MGC:5835 IMAGE:3489939) mm
RG-U34C:RC_AI172143_AT phytanoyl-CoA hydroxylase rn
RAE230A:1387271_AT phytanoyl-CoA hydroxylase rn
RAT230_2:1387271_AT phytanoyl-CoA hydroxylase rn
RAE230B:1393245_AT Phytanoyl-CoA hydroxylase rn
RAT230_2:1393245_AT Phytanoyl-CoA hydroxylase rn
RG-U34B:RC_AA899815_AT Phytanoyl-CoA hydroxylase rn
RG-U34B:RC_AI029057_AT Phytanoyl-CoA hydroxylase rn
Protein Similarities
Method ID Description E-Value
blast CAB02322 Hypothetical protein ZK550.6 [Caenorhabditis elegans] emb|CAB05318.1| Hypothetical protein ZK550.6 [Caenorhabditis elegans] ref|NP_503062.1| ZK550.6 [Caenorhabditis elegans] sp|O62515|PAHX_CAEEL Probable phytanoyl-CoA dioxygenase (Phytanoyl-CoA alpha-hydroxylase) (PhyH) (Phytanic acid oxidase) 0.0
blast CAE57415 Hypothetical protein CBG00370 [Caenorhabditis briggsae] 1.0E-177
blast AAH86573 Phytanoyl-CoA hydroxylase [Rattus norvegicus] ref|NP_446126.1| phytanoyl-CoA hydroxylase [Rattus norvegicus] sp|P57093|PAHX_RAT Phytanoyl-CoA dioxygenase, peroxisomal precursor (Phytanoyl-CoA alpha-hydroxylase) (PhyH) (Phytanic acid oxidase) gb|AAF15971.1| peroxisomal phytanoyl-CoA hydroxylase [Rattus norvegicus] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR008775 EMBL-EBI Phytanoyl-CoA dioxygenase 5.2E-78
Sequence
Target Sequence 
>C. ELEGANS:186811_S_AT
aagatcttcaatatttcccattccgtccagcagattttatttgttgtgcttggaccgcaa
tggagaagatcacaagagcaaatggatgtctggttgttgttcccggaactcataaaggtg
ttcttttgccacacgagtacccgaaatgggagggtggtgtgaacaaagcctatcacggaa
tccaggactatgacacgtcgacgccacgtatccacgtggaaatggaacccggagataccg
tattctttcatccaattcttattcatggttccggagccaatagaactgaaggattccgca
aagcgatctcctgtcactacgcaaatgacgacctctgccgttatgtaaacgtcgagggta
caactcaagagacattggcagaggagatcatcgaaatcgccaagaagcgtctgacaagat
atggtctcgacccgaatactgtaaccttggatttcgcggacatttggagggttcgcgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGATCTTCAATATTTCCCATTCCG 428 155 440 Antisense
CCATTCCGTCCAGCAGATTTTATTT 239 271 457 Antisense
GTGTTCTTTTGCCACACGAGTACCC 310 483 557 Antisense
TCCAGGACTATGACACGTCGACGCC 295 587 620 Antisense
GGAGATACCGTATTCTTTCATCCAA 99 519 670 Antisense
CAATTCTTATTCATGGTTCCGGAGC 590 177 692 Antisense
GATCTCCTGTCACTACGCAAATGAC 189 419 744 Antisense
CAAATGACGACCTCTGCCGTTATGT 78 193 761 Antisense
GGTCTCGACCCGAATACTGTAACCT 503 395 862 Antisense
GTAACCTTGGATTTCGCGGACATTT 99 463 880 Antisense
GCGGACATTTGGAGGGTTCGCGCCA 200 291 895 Antisense
Sequence Source Affymetrix Proprietary Database