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[186810_at]

Description F43D9.2 /REP_DB=WormBase Gene ID /WP=CE00992 /TR=SW:Q20365 /GB=CAA84705.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 27      4grp_total > 56      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 86.4
(1.8)
A 		92.2
(3)
A 		88.2
(2.2)
A 		62.4
(-3)
A 		30
B 57
(-1.8)
A 		45.9
(-3)
A 		103
(3)
A 		65.7
(-0.9)
A 		57
C 78
(-2.6)
A 		99.4
(3)
A 		89.6
(0.4)
A 		76.6
(-3)
A 		23
avr 73.8
(-1.7) 		79.2
(-0.4) 		93.6
(3) 		68.2
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 103.6
(2.3)
A 		90.1
(1.4)
A 		65.2
(-0.4)
A 		28.4
(-3)
A 		54.3
(-1.2)
A 		113.2
(3)
A 		85
B 79.2
(0.7)
A 		67.3
(-0.6)
A 		102.1
(3)
A 		68.6
(-0.4)
A 		43.6
(-3)
A 		72.1
(-0.1)
A 		59
C 149.8
(3)
A 		64.4
(-0.5)
A 		16.8
(-2.5)
A 		4.4
(-3)
A 		54.6
(-0.9)
A 		72
(-0.2)
A 		145
avr 110.9
(3) 		73.9
(0.1) 		61.4
(-0.9) 		33.8
(-3) 		50.8
(-1.7) 		85.8
(1) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 31.5
(3)
A 		20
(-0.1)
A 		23.1
(0.8)
A 		9.1
(-3)
A 		22
B 18.2
(0.3)
A 		11.3
(-3)
A 		23.9
(3)
A 		14
(-1.7)
A 		13
C 25.8
(1.2)
A 		28.6
(2.1)
A 		31.2
(3)
A 		12.9
(-3)
A 		18
avr 25.2
(2.6) 		20
(0.4) 		26.1
(3) 		12
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 19.1
(-0.1)
A 		32
(3)
A 		14.2
(-1.3)
A 		7.2
(-3)
A 		16.4
(-0.8)
A 		27.6
(1.9)
A 		25
B 27.6
(3)
A 		22.4
(-0.2)
A 		24.8
(1.3)
A 		18.2
(-2.7)
A 		17.7
(-3)
A 		26.9
(2.6)
A 		10
C 55.5
(3)
A 		19.6
(-0.9)
A 		4.3
(-2.6)
A 		0.9
(-3)
A 		17.7
(-1.2)
A 		24.1
(-0.5)
A 		55
avr 34.1
(3) 		24.7
(0.8) 		14.4
(-1.7) 		8.8
(-3) 		17.3
(-1) 		26.2
(1.1) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186810_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6800
Sequence Type Exemplar sequence
Representative Public ID F43D9.2 NCBI
Target Description F43D9.2 /REP_DB=WormBase Gene ID /WP=CE00992 /TR=SW:Q20365 /GB=CAA84705.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066913(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066913 NCBI Caenorhabditis elegans RAB family member (rab-33) (rab-33) mRNA, complete cds. 11/11 None
SNAP00000024069 ENSEMBL cdna:SNAP chromosome:CEL140:III:10500455:10503732:-1 11/11 None
GENEFINDER00000024077 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10500455:10503732:-1 11/11 None
F43D9.2 ENSEMBL cdna:known chromosome:CEL140:III:10500455:10503732:-1 gene:F43D9.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
U68264 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10500450-10503728(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rab-33
Ensembl F43D9.2
Entrez Gene 176470 Entrez gene
SwissProt Q20365 EMBL-EBI
WormBase CE00992 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.2252.1.S1_S_AT RAB33B, member RAS oncogene family gga
CHICKEN:GGAAFFX.13136.1.S1_AT RAB33B, member RAS oncogene family gga
HG-U133_PLUS_2:221014_S_AT RAB33B, member RAS oncogene family hs
HG-U133A:221014_S_AT RAB33B, member RAS oncogene family hs
HG-U133A_2:221014_S_AT RAB33B, member RAS oncogene family hs
U133_X3P:G13786128_3P_S_AT RAB33B, member RAS oncogene family hs
HG-U95E:85186_R_AT RAB33B, member RAS oncogene family hs
U133_X3P:HS.224914.0.A1_3P_AT RAB33B, member RAS oncogene family hs
HG-U133_PLUS_2:241388_AT RAB33B, member RAS oncogene family hs
HG-U133B:241388_AT RAB33B, member RAS oncogene family hs
MG-U74AV2:97058_F_AT RAB33B, member of RAS oncogene family mm
MU11KSUBA:AB004664_AT RAB33B, member of RAS oncogene family mm
MU11KSUBA:AB004664_G_AT RAB33B, member of RAS oncogene family mm
MG-U74AV2:103062_AT RAB33B, member of RAS oncogene family mm
MOE430A:1423083_AT RAB33B, member of RAS oncogene family mm
MOUSE430A_2:1423083_AT RAB33B, member of RAS oncogene family mm
MOUSE430_2:1423083_AT RAB33B, member of RAS oncogene family mm
MG-U74CV2:137239_AT RAB33B, member of RAS oncogene family (Rab33b), mRNA mm
MU19KSUBC:TC40604_I_AT RAB33B, member of RAS oncogene family (Rab33b), mRNA mm
MU19KSUBC:TC40604_S_AT RAB33B, member of RAS oncogene family (Rab33b), mRNA mm
RAE230B:1394665_AT RAB33B, member of RAS oncogene family (predicted) rn
RAT230_2:1394665_AT RAB33B, member of RAS oncogene family (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84705 Hypothetical protein F43D9.2 [Caenorhabditis elegans] ref|NP_499314.1| RAB family member (rab-33) [Caenorhabditis elegans] sp|Q20365|RAB33_CAEEL Putative Ras-related protein Rab-33 1.0E-163
blast CAE71456 Hypothetical protein CBG18374 [Caenorhabditis briggsae] 1.0E-142
blast AAH36064 RAB33B, member RAS oncogene family [Homo sapiens] 8.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 6.7E-56
Sequence
Target Sequence 
>C. ELEGANS:186810_AT
aatcatagtgggcaacgcggccgtcggaaaaacgtgtctttcattccgtttttgttgtgg
aagattccccgaacatactgaagccacaatcggagtggattttcgagaaagaagttgtgt
aattgaaaatgaacttcttcgtgttcagctatgggatactgctggacaggaaagatatcg
acaatcgattgttgctcattattatagaaatgtgaatgctgtcgtttttgtgtatgatgt
cacatgccgtgagtcattcaacgatttggcactttggataaaagaatgtgaaaagcatgg
tctcgttggggatagtgaagttccgaggatcctgatcggcaacaagtgtgacgtggaatg
cacaaatcgagtgtccactgatgaagctcagatgttcgccgaccgtaacaacatggcatt
attcgaaacatcagccaaattagcatcagaagcagatcatgtcgaatcaattttcctcac
actgttaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCATAGTGGGCAACGCGGCCGTC 506 169 321 Antisense
TGTCTTTCATTCCGTTTTTGTTGTG 302 559 355 Antisense
ATTCCCCGAACATACTGAAGCCACA 566 7 384 Antisense
ACTTCTTCGTGTTCAGCTATGGGAT 673 101 453 Antisense
GTGAATGCTGTCGTTTTTGTGTATG 515 481 532 Antisense
GATGTCACATGCCGTGAGTCATTCA 373 409 556 Antisense
AAGCATGGTCTCGTTGGGGATAGTG 258 151 613 Antisense
GGATCCTGATCGGCAACAAGTGTGA 378 513 647 Antisense
TGATGAAGCTCAGATGTTCGCCGAC 114 567 699 Antisense
GTTCGCCGACCGTAACAACATGGCA 17 443 714 Antisense
AATCAATTTTCCTCACACTGTTACA 369 171 785 Antisense
Sequence Source Affymetrix Proprietary Database