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[186803_at]

Description Y45G12C.8 /REP_DB=WormBase Gene ID /WP=CE21941 /TR=Q9N4Y7 /GB=AAF60590.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 26      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 3.1
(-3)
A 		15.1
(1.7)
A 		15.1
(1.7)
A 		18.4
(3)
A 		15
B 9.5
(-3)
A 		68.1
(3)
A 		9.2
(-3)
A 		16.7
(-2.2)
A 		59
C 6.4
(-2.7)
A 		12.2
(-2.3)
A 		2.1
(-3)
A 		86.9
(3)
A 		85
avr 6.3
(-3) 		31.8
(1.4) 		8.8
(-2.6) 		40.7
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 42.7
(3)
A 		8.2
(-2.9)
A 		8.1
(-2.9)
A 		7.5
(-3)
A 		10.2
(-2.5)
A 		35.2
(1.7)
A 		35
B 11.9
(-1)
A 		11.3
(-1.1)
A 		10.9
(-1.2)
A 		30.2
(3)
A 		7.3
(-2)
A 		2.7
(-3)
A 		28
C 7.9
(-2)
A 		12.1
(-1.2)
A 		11.4
(-1.3)
A 		18.4
(-0.1)
A 		35.3
(3)
A 		2.1
(-3)
A 		33
avr 20.8
(3) 		10.5
(-2.8) 		10.1
(-3) 		18.7
(1.8) 		17.6
(1.2) 		13.3
(-1.2) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 1.1
(-3)
A 		3.3
(1.7)
A 		3.9
(3)
A 		2.7
(0.4)
A 		3
B 3
(-2.6)
A 		16.8
(3)
A 		2.1
(-3)
A 		3.6
(-2.4)
A 		15
C 2.1
(-2.4)
A 		3.5
(-1.8)
A 		0.7
(-3)
A 		14.7
(3)
A 		14
avr 2.1
(-3) 		7.9
(3) 		2.2
(-2.9) 		7
(2.1) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 7.9
(2.4)
A 		2.9
(-2)
A 		1.8
(-3)
A 		1.9
(-2.9)
A 		3.1
(-1.9)
A 		8.6
(3)
A 		7
B 4.1
(-0.3)
A 		3.8
(-0.6)
A 		2.6
(-1.6)
A 		8
(3)
A 		3
(-1.3)
A 		1
(-3)
A 		7
C 2.9
(-1.8)
A 		3.7
(-1.3)
A 		2.9
(-1.8)
A 		3.7
(-1.3)
A 		11.4
(3)
A 		0.7
(-3)
A 		11
avr 5
(1.6) 		3.5
(-1.1) 		2.4
(-3) 		4.5
(0.7) 		5.8
(3) 		3.4
(-1.2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186803_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15489
Sequence Type Exemplar sequence
Representative Public ID Y45G12C.8 NCBI
Target Description Y45G12C.8 /REP_DB=WormBase Gene ID /WP=CE21941 /TR=Q9N4Y7 /GB=AAF60590.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071289(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071289 NCBI Caenorhabditis elegans Serpentine Receptor, class BC (class B-like) family member (srbc-81) (srbc-81) mRNA, complete cds. 9/11 None
Y45G12C.8 ENSEMBL cdna:known chromosome:CEL140:V:2522261:2524118:1 gene:Y45G12C.8 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022914 4/11 Cross Hyb Matching Probes None
SNAP00000043307 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2413981-2415827(+) 77.6 77.6
chrV:2522260-2524106(+) 77.6 77.6
Public Domain and Genome References
Gene Title
Gene Symbol srbc-81
Ensembl Y45G12C.8
Entrez Gene 189936 Entrez gene
SwissProt Q9N4Y7 EMBL-EBI
WormBase CE32259 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503690 Serpentine Receptor, class BC (class B-like) family member (srbc-81) [Caenorhabditis elegans] gb|AAF60590.2| Hypothetical protein Y45G12C.8 [Caenorhabditis elegans] 1.0E-145
blast CAB05561 Hypothetical protein M01B2.3 [Caenorhabditis elegans] ref|NP_506775.1| Serpentine Receptor, class BC (class B-like) family member (srbc-75) [Caenorhabditis elegans] 1.0E-61
blast CAB03794 Hypothetical protein B0250.6 [Caenorhabditis elegans] ref|NP_507942.1| Serpentine Receptor, class BC (class B-like) family member (srbc-79) [Caenorhabditis elegans] 4.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 8.3E-77
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503690.2 7 10-32,45-67,94-116,123-145,173-195,215-234,244-263
Y45G12C.8 7 10-32,45-67,94-116,123-145,173-195,215-234,244-263
Sequence
Target Sequence 
>C. ELEGANS:186803_AT
accgcttcttttgtgccataaacagctgttactatttttattgggtcttgcatgagcaaa
ctgttggctgtttaaatgttgtattttccctgattctttgcattcgaattctcagttccc
actattcttcaaaaacgcaaacaaataagaaactgtctatagctacccgcattgcacttc
ttaatgcgttcatagttttcttcttcaatatcattccgtcatacgcgtttgccaatttca
caagtgccaatattgaggtggctggaccaatggcaacagttaccaagacacttggatttg
taattgagggattaataactcgtcgtgtattgtttcggaagcctcgcgtctcacccggtg
ttgcttcaccaaatgtagtaaatttcaattttaatcggcaaaattgtacgcatcctataa
atgttcctacatctattttaaaaagcaagaaaattctattaaaatatcaaaaaaaaacgg
gggaaaaaatttcaaaaaggcacatttttaagtgtttccgtctcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCGCTTCTTTTGTGCCATAAACAG 629 89 485 Antisense
GTATTTTCCCTGATTCTTTGCATTC 297 453 565 Antisense
TTGCATTCGAATTCTCAGTTCCCAC 32 705 582 Antisense
CAGTTCCCACTATTCTTCAAAAACG 513 203 597 Antisense
AAGAAACTGTCTATAGCTACCCGCA 214 153 631 Antisense
CTTCAATATCATTCCGTCATACGCG 438 221 687 Antisense
GTCATACGCGTTTGCCAATTTCACA 506 467 702 Antisense
CGTGTATTGTTTCGGAAGCCTCGCG 217 245 808 Antisense
TCACCCGGTGTTGCTTCACCAAATG 187 625 835 Antisense
GTACGCATCCTATAAATGTTCCTAC 546 457 890 Antisense
CATTTTTAAGTGTTTCCGTCTCATA 589 215 987 Antisense
Sequence Source Affymetrix Proprietary Database