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[186790_at]

Description C07B5.5 /REP_DB=WormBase Gene ID /WP=CE00895 /TR=SW:Q17778 /GB=CAA86412.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 62      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 25      6grp_total > 62      4grp_total > 53      6grp_highest_sub > 03      6grp_cgc > 93      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2762
1 2 3 4 range
A 3247.9
(3)
P 		2748.4
(1.9)
P 		2310.6
(0.9)
P 		577.9
(-3)
P 		2670
B 3031.4
(3)
P 		2227.6
(1.1)
P 		2324.4
(1.3)
P 		486.1
(-3)
P 		2545
C 3202.9
(3)
P 		2291.4
(0.9)
P 		1953.5
(0.1)
P 		641.8
(-3)
P 		2561
avr 3160.7
(3) 		2422.5
(1.3) 		2196.2
(0.8) 		568.6
(-3) 		2592

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4049
1 2 3 4 5 6 range
A 3653.9
(-1.7)
P 		3606.9
(-1.8)
P 		6608.6
(3)
P 		5477.2
(1.2)
P 		3716.4
(-1.6)
P 		2820.3
(-3)
P 		3788
B 3112.8
(-2.7)
P 		3761.9
(-1.5)
P 		6236.9
(3)
P 		4865.7
(0.5)
P 		3773
(-1.5)
P 		2936
(-3)
P 		3301
C 2979.9
(-2.2)
P 		3540.8
(-1.2)
P 		5827.6
(3)
P 		5704
(2.8)
P 		4223
(0.1)
P 		2559.3
(-3)
P 		3268
avr 3248.9
(-2.2) 		3636.5
(-1.5) 		6224.4
(3) 		5349
(1.5) 		3904.1
(-1) 		2771.9
(-3) 		3453
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1098
1 2 3 4 range
A 1182.5
(3)
P 		596.6
(-0.2)
P 		604
(-0.2)
P 		84.6
(-3)
P 		1098
B 966.7
(3)
P 		550.2
(0.1)
P 		538.6
(0)
P 		103.6
(-3)
P 		863
C 1060.3
(3)
P 		658.8
(0.5)
P 		680.8
(0.6)
P 		108.2
(-3)
P 		952
avr 1069.8
(3) 		601.9
(0.1) 		607.8
(0.1) 		98.8
(-3) 		971

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 859
1 2 3 4 5 6 range
A 673.6
(-3)
P 		1282.8
(1.8)
P 		1439.2
(3)
P 		1389.2
(2.6)
P 		1121.4
(0.5)
P 		686.7
(-2.9)
P 		766
B 1083.1
(-3)
P 		1252.2
(-0.7)
P 		1514.5
(2.8)
P 		1288.8
(-0.3)
P 		1532.9
(3)
P 		1094.8
(-2.8)
P 		450
C 1104.5
(-0.6)
P 		1080.4
(-0.9)
P 		1483.2
(3)
P 		1147.1
(-0.2)
P 		1366.5
(1.9)
P 		856.8
(-3)
P 		626
avr 953.7
(-2.3) 		1205.1
(0.3) 		1479
(3) 		1275
(1) 		1340.3
(1.6) 		879.4
(-3) 		600
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186790_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16199
Sequence Type Exemplar sequence
Representative Public ID C07B5.5 NCBI
Target Description C07B5.5 /REP_DB=WormBase Gene ID /WP=CE00895 /TR=SW:Q17778 /GB=CAA86412.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077203(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077203 NCBI Caenorhabditis elegans abnormal NUClease family member (nuc-1) (nuc-1) mRNA, complete cds. 11/11 None
SNAP00000005688 ENSEMBL cdna:SNAP chromosome:CEL140:X:9539878:9542181:1 11/11 None
GENEFINDER00000005691 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9539878:9542181:1 11/11 None
C07B5.5 ENSEMBL cdna:known chromosome:CEL140:X:9539843:9542181:1 gene:C07B5.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9539877-9542181(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nuc-1
Ensembl C07B5.5
Entrez Gene 181174 Entrez gene
SwissProt Q17778 EMBL-EBI
WormBase CE00895 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590152_AT similar to deoxyribonuclease II beta isoform 1 precursor cfa
CANINE_2:CFA.13294.1.A1_AT similar to deoxyribonuclease II beta isoform 1 precursor cfa
CANINE_2:CFAAFFX.31105.1.S1_S_AT similar to deoxyribonuclease II beta isoform 1 precursor cfa
DROSOPHILA_2:1640898_AT Deoxyribonuclease II dm
DROSGENOME1:152336_AT Deoxyribonuclease II dm
CHICKEN:GGA.16501.1.S1_AT deoxyribonuclease II beta gga
HG-U133_PLUS_2:220380_AT deoxyribonuclease II beta hs
HG-U133A:220380_AT deoxyribonuclease II beta hs
HG-FOCUS:220380_AT deoxyribonuclease II beta hs
HG-U133A_2:220380_AT deoxyribonuclease II beta hs
U133_X3P:G10864044_3P_AT deoxyribonuclease II beta hs
HG-U95E:74414_AT deoxyribonuclease II beta hs
MOUSE430A_2:1450237_AT deoxyribonuclease II beta mm
MOUSE430_2:1450237_AT deoxyribonuclease II beta mm
MOE430A:1450237_AT deoxyribonuclease II beta mm
MG-U74BV2:104878_AT deoxyribonuclease II beta mm
MG-U74BV2:114863_AT deoxyribonuclease II beta mm
RAE230A:1368491_AT DNaseII-like acid DNase rn
RAT230_2:1368491_AT DNaseII-like acid DNase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6259 DNA metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4531 deoxyribonuclease II activity inferred from electronic annotation QuickGO AmiGO
4520 endodeoxyribonuclease activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86412 Hypothetical protein C07B5.5 [Caenorhabditis elegans] ref|NP_509604.1| abnormal NUClease family member (nuc-1) [Caenorhabditis elegans] sp|Q17778|NUC1_CAEEL Deoxyribonuclease II precursor (DNase II) 0.0
blast CAA86412 Hypothetical protein C07B5.5 [Caenorhabditis elegans] ref|NP_509604.1| abnormal NUClease family member (nuc-1) [Caenorhabditis elegans] sp|Q17778|NUC1_CAEEL Deoxyribonuclease II precursor (DNase II) 1.0E-165
blast BAD13432.1 plancitoxin I [Acanthaster planci] 9.0E-57
blast CAE65227 Hypothetical protein CBG10104 [Caenorhabditis briggsae] 6.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR004947 EMBL-EBI Deoxyribonuclease II 1.0E-126
Pfam IPR004947 EMBL-EBI Deoxyribonuclease II 2.4E-41
Pfam IPR004947 EMBL-EBI Deoxyribonuclease II 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:186790_AT
gaatcttccaacgaacatggctgctgacaatgcagatttggctaaagcaatcgctggaca
ataccaaaaaggacagccattccaaagcgttattgagttggagactatggctggatattc
gtttactaattttgccaagtcgaaggaattcaatgcagatctatacgatactcttgtggc
tccaacactgaaaacagatttggtcgttgaaacatggagacgtggaagtgagatcccact
tgactgcaaactaacctaccacgccaatgatgctctgtcaattcacgttggtagtacaac
cgcgttctcatacaccaaagaccactcgaaaatggctcactcagctgatatgacaaagcc
gtgggtgtgcatcggagatatcaaccgaatgacatctcaatatgtacgcggaggaggtac
tacttgtatcagcagctcgttcctttggaaagccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCTTCCAACGAACATGGCTGCT 213 329 651 Antisense
AGGACAGCCATTCCAAAGCGTTATT 609 55 720 Antisense
CTATACGATACTCTTGTGGCTCCAA 19 225 811 Antisense
TGAGATCCCACTTGACTGCAAACTA 137 567 879 Antisense
TAACCTACCACGCCAATGATGCTCT 468 637 902 Antisense
TTGGTAGTACAACCGCGTTCTCATA 226 695 938 Antisense
GTTCTCATACACCAAAGACCACTCG 331 443 954 Antisense
GACCACTCGAAAATGGCTCACTCAG 164 385 970 Antisense
ATGACAAAGCCGTGGGTGTGCATCG 167 43 1000 Antisense
GTACTACTTGTATCAGCAGCTCGTT 613 459 1067 Antisense
GCAGCTCGTTCCTTTGGAAAGCCTA 217 311 1082 Antisense
Sequence Source Affymetrix Proprietary Database