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[186780_at]

Description F10B5.2 /REP_DB=WormBase Gene ID /WP=CE01544 /TR=SW:Q09305 /GB=CAA88309.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 87      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 94      6grp_total > 87      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 216
1 2 3 4 range
A 488.8
(3)
P 		375.1
(-0.2)
P 		273.1
(-3)
P 		416.3
(1)
P 		216
B 394.3
(3)
P 		317.8
(-3)
P 		378.2
(1.7)
P 		389.8
(2.6)
P 		77
C 403.5
(2.9)
P 		354.3
(-1.5)
P 		405.2
(3)
P 		337.2
(-3)
P 		68
avr 428.9
(3) 		349.1
(-3) 		352.2
(-2.8) 		381.1
(-0.6) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 518
1 2 3 4 5 6 range
A 547
(-0.3)
P 		364.2
(-2.6)
P 		329.2
(-3)
P 		473
(-1.2)
P 		672.1
(1.2)
P 		815
(3)
P 		486
B 441.4
(-1.4)
P 		318.3
(-3)
P 		326.5
(-2.9)
P 		445.2
(-1.4)
P 		525
(-0.3)
P 		786.2
(3)
P 		468
C 315.7
(-2.8)
P 		305.8
(-2.9)
P 		296.6
(-3)
P 		448.9
(-1.2)
P 		510.1
(-0.4)
P 		797.3
(3)
P 		501
avr 434.7
(-1.5) 		329.4
(-2.9) 		317.4
(-3) 		455.7
(-1.3) 		569.1
(0.1) 		799.5
(3) 		482
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 178
(3)
P 		81.4
(-1.9)
P 		71.4
(-2.5)
P 		60.9
(-3)
P 		117
B 125.7
(3)
P 		78.5
(-3)
P 		87.6
(-1.8)
P 		83.1
(-2.4)
P 		47
C 133.6
(2.5)
P 		101.9
(0.2)
P 		141.2
(3)
P 		56.9
(-3)
P 		84
avr 145.8
(3) 		87.3
(-1.5) 		100.1
(-0.5) 		67
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 100.8
(-1.7)
P 		129.5
(-0.4)
P 		71.7
(-3)
P 		120
(-0.8)
P 		202.8
(3)
P 		198.4
(2.8)
P 		131
B 153.6
(-0.9)
P 		105.9
(-2.3)
P 		79.3
(-3)
P 		117.9
(-1.9)
P 		213.3
(0.8)
P 		293.2
(3)
P 		214
C 117
(-1.7)
P 		93.3
(-2.4)
P 		75.5
(-3)
P 		90.3
(-2.5)
P 		165.1
(-0.2)
P 		266.9
(3)
P 		191
avr 123.8
(-1.4) 		109.6
(-1.8) 		75.5
(-3) 		109.4
(-1.9) 		193.7
(1) 		252.8
(3) 		177
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186780_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3156
Sequence Type Exemplar sequence
Representative Public ID F10B5.2 NCBI
Target Description F10B5.2 /REP_DB=WormBase Gene ID /WP=CE01544 /TR=SW:Q09305 /GB=CAA88309.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063307(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063307 NCBI Caenorhabditis elegans F10B5.2 (F10B5.2) mRNA, complete cds. 11/11 None
SNAP00000014813 ENSEMBL cdna:SNAP chromosome:CEL140:II:8144683:8147071:1 11/11 None
GENEFINDER00000014829 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8144683:8147071:1 11/11 None
F10B5.2 ENSEMBL cdna:known chromosome:CEL140:II:8144722:8147071:1 gene:F10B5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8144718-8147068(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F10B5.2
Entrez Gene 184284 Entrez gene
SwissProt Q09305 EMBL-EBI
WormBase CE01544 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256363_AT AAR2 protein family at
CANINE:1602948_AT similar to Protein C20orf4 homolog cfa
CANINE_2:CFA.6905.1.A1_AT similar to Protein C20orf4 homolog cfa
CANINE_2:CFAAFFX.13371.1.S1_S_AT similar to Protein C20orf4 homolog cfa
DROSOPHILA_2:1639409_AT dm
DROSGENOME1:150123_AT dm
HG-U133_PLUS_2:218089_AT chromosome 20 open reading frame 4 hs
HG-U133A:218089_AT chromosome 20 open reading frame 4 hs
HG-U133A_2:218089_AT chromosome 20 open reading frame 4 hs
HU35KSUBA:RC_AA465214_AT chromosome 20 open reading frame 4 hs
U133_X3P:G7661627_3P_AT chromosome 20 open reading frame 4 hs
HG-U95B:48503_AT chromosome 20 open reading frame 4 hs
HG-U95D:80922_AT chromosome 20 open reading frame 4 hs
U133_X3P:HS.306898.0.S1_3P_AT Chromosome 20 open reading frame 4 hs
HG-U133_PLUS_2:234654_AT Chromosome 20 open reading frame 4 hs
HG-U133B:234654_AT Chromosome 20 open reading frame 4 hs
HG-U133_PLUS_2:234181_AT Chromosome 20 open reading frame 4 hs
HG-U133B:234181_AT Chromosome 20 open reading frame 4 hs
MOE430A:1426017_A_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430_2:1426017_A_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430A_2:1426017_A_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430_2:1459456_AT RIKEN cDNA 0610011L14 gene mm
MOE430B:1459456_AT RIKEN cDNA 0610011L14 gene mm
MG-U74BV2:109678_AT RIKEN cDNA 0610011L14 gene mm
MG-U74BV2:164765_AT RIKEN cDNA 0610011L14 gene mm
MOE430A:1437835_A_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430A_2:1437835_A_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430_2:1437835_A_AT RIKEN cDNA 0610011L14 gene mm
MOE430A:1437836_X_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430A_2:1437836_X_AT RIKEN cDNA 0610011L14 gene mm
MOUSE430_2:1437836_X_AT RIKEN cDNA 0610011L14 gene mm
MU19KSUBC:TC40421_AT RIKEN cDNA 0610011L14 gene (0610011L14Rik), mRNA mm
RAT230_2:1378760_AT similar to RIKEN cDNA 0610011L14 gene (predicted) rn
RAE230B:1378760_AT similar to RIKEN cDNA 0610011L14 gene (predicted) rn
RAE230B:1395383_AT similar to RIKEN cDNA 0610011L14 gene (predicted) rn
RAT230_2:1395383_AT similar to RIKEN cDNA 0610011L14 gene (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA88309 Hypothetical protein F10B5.2 [Caenorhabditis elegans] ref|NP_495708.1| F10B5.2 [Caenorhabditis elegans] sp|Q09305|YQQ2_CAEEL Hypothetical protein F10B5.2 in chromosome II 0.0
blast CAE57732 Hypothetical protein CBG00743 [Caenorhabditis briggsae] 0.0
blast XP_796079 PREDICTED: similar to Protein C20orf4 homolog [Strongylocentrotus purpuratus] 4.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR007946 EMBL-EBI AAR2 1.0E-126
Pfam IPR007946 EMBL-EBI AAR2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:186780_AT
cagagctcgtcagccttgagactgagttcatggaaaatgcagaaaaagagcacaaagaca
gtcattttcgaaatcgagtggatcgagagaatccagttcggacgagattcacagatcagc
atggattaccgattatgaagattcgagagggatacgaaattcgattccaagatataccac
ctctcacggttagccagaatcgagtaggaattgaatattctgatcgtctttaccgtcttc
ttcgcgctctcggtggtgattggaagcaattgctggctgaaatgcaaattgcatttgttt
gttttctgcagggacaagtttttgaaggattcgagcaatggaaacgaattattcatttga
tgtcgtgttgtccaaattcattaggctctgagaaagagttgttcatgtcatttatcagag
ttctcttcttccaactcaaagaatgccccactgactttttcgtcgatattgtttctcgtg
acaactttttgacaactactctctcaatgttgttcgccaacgtccgagactctgcacacg
ccggtgatgatttgaaaaagaagactgcccaattcaaacagtatctcacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGAGCTCGTCAGCCTTGAGACTGA 63 207 464 Antisense
CGATTCCAAGATATACCACCTCTCA 403 247 625 Antisense
ACCTCTCACGGTTAGCCAGAATCGA 617 87 642 Antisense
ATATTCTGATCGTCTTTACCGTCTT 360 19 678 Antisense
CGCGCTCTCGGTGGTGATTGGAAGC 221 257 706 Antisense
GTGTTGTCCAAATTCATTAGGCTCT 207 485 828 Antisense
TTATCAGAGTTCTCTTCTTCCAACT 170 681 875 Antisense
AAAGAATGCCCCACTGACTTTTTCG 337 119 901 Antisense
ACAACTTTTTGACAACTACTCTCTC 365 111 944 Antisense
ACTCTGCACACGCCGGTGATGATTT 74 101 992 Antisense
TGCCCAATTCAAACAGTATCTCACG 299 585 1029 Antisense
Sequence Source Affymetrix Proprietary Database