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[186756_s_at]

Description Y53C12B.3A /REP_DB=WormBase Gene ID /WP=CE19224 /TR=O18218 /GB=CAB16493.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 79      4grp_highest_8_8 > 11      4grp_high_8_8 > 55      6grp_total > 79      4grp_total > 48      6grp_cgc > 29      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 208
1 2 3 4 range
A 292.7
(-3)
P 		314.8
(-2)
P 		424.5
(3)
P 		367.7
(0.4)
P 		132
B 295.9
(0.1)
P 		368.3
(3)
P 		361.7
(2.7)
P 		216.9
(-3)
P 		151
C 279.8
(-0.6)
P 		284.1
(0.2)
P 		266.6
(-3)
P 		299.6
(3)
P 		33
avr 289.5
(-3) 		322.4
(0.2) 		350.9
(3) 		294.7
(-2.5) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 632
1 2 3 4 5 6 range
A 209.9
(-2.3)
P 		332.4
(-0.7)
P 		161.8
(-3)
P 		213
(-2.3)
P 		197
(-2.5)
P 		603.9
(3)
P 		442
B 256.8
(-0.2)
P 		211.6
(-1)
P 		187.2
(-1.4)
P 		84.7
(-3)
A 		192.2
(-1.3)
P 		459.2
(3)
P 		375
C 357
(-0.6)
P 		339.3
(-0.8)
P 		249.9
(-1.7)
P 		121.8
(-3)
P 		716.3
(3)
P 		382.3
(-0.4)
P 		595
avr 274.6
(-0.6) 		294.4
(-0.3) 		199.6
(-2) 		139.8
(-3) 		368.5
(1) 		481.8
(3) 		342
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 106.6
(2.5)
P 		68.3
(-1.5)
P 		111
(3)
P 		53.8
(-3)
P 		57
B 94.4
(3)
P 		91
(2.6)
P 		83.8
(1.7)
P 		46.2
(-3)
P 		48
C 92.6
(3)
P 		81.7
(1.4)
P 		92.9
(3)
P 		50.5
(-3)
P 		42
avr 97.9
(3) 		80.3
(0.8) 		95.9
(2.7) 		50.2
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 38.7
(-2.8)
P 		118.2
(1.5)
P 		35.2
(-3)
P 		54
(-2)
P 		59.5
(-1.7)
P 		147
(3)
P 		112
B 89.4
(-0.3)
P 		70.4
(-1.1)
P 		45.4
(-2.1)
P 		22.4
(-3)
A 		78.1
(-0.8)
P 		171.2
(3)
P 		149
C 132.3
(0.1)
P 		103.5
(-0.7)
P 		63.6
(-1.9)
P 		24.5
(-3)
P 		231.8
(3)
P 		128
(-0)
P 		207
avr 86.8
(-0.2) 		97.4
(0.3) 		48.1
(-2.2) 		33.6
(-3) 		123.1
(1.7) 		148.7
(3) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186756_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3890
Sequence Type Exemplar sequence
Representative Public ID Y53C12B.3A NCBI
Target Description Y53C12B.3A /REP_DB=WormBase Gene ID /WP=CE19224 /TR=O18218 /GB=CAB16493.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063701(11), NM_063700(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063701 NCBI Caenorhabditis elegans NanOS related family member (nos-3) (nos-3) mRNA, complete cds. 11/11 None
NM_063700 NCBI Caenorhabditis elegans NanOS related family member (nos-3) (nos-3) mRNA, complete cds. 11/11 None
SNAP00000006654 ENSEMBL cdna:SNAP chromosome:CEL140:II:9746645:9749781:1 11/11 None
GENEFINDER00000006680 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9746645:9750141:1 11/11 None
Y53C12B.3a ENSEMBL cdna:known chromosome:CEL140:II:9746629:9750354:1 gene:Y53C12B.3 11/11 None
Y53C12B.3b ENSEMBL cdna:known chromosome:CEL140:II:9746645:9750354:1 gene:Y53C12B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9746641-9749768(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y53C12B.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB16493 Hypothetical protein Y53C12B.3a [Caenorhabditis elegans] ref|NP_496102.1| NanOS related family member (nos-3) [Caenorhabditis elegans] 1.0E-127
blast CAB16492 Hypothetical protein Y53C12B.3b [Caenorhabditis elegans] ref|NP_496101.1| NanOS related family member (nos-3) [Caenorhabditis elegans] 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 7.2E-26
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 7.2E-26
Sequence
Target Sequence 
>C. ELEGANS:186756_S_AT
gaacattaccagttgcctgctgaagaagtgtcttcccataatattcgcaaggataatggt
gatttgtggtgtgagcatatgaaaaagatcaaatgcgggcattgtgaggcgactggagaa
caaggtcatcaccctctaatttgtccaaaaaagaaagaagaagaacgtgtggcaaaatct
cgagagtcttcgcagaagccgatcgatccgcaggaaataagtgatgatcaggatgatact
gtaccggatgttcccgatcaaatagtcgaacaagacaatcagtcacataaatctcgtcac
aattcagaaaatcgcagtggcggtagaggatacggtcagaactatagccatagttatggc
ggaaattctactggtagctatcgtggacgatcgtctggaggaagtggaggcagtggacgt
ggtggtggtcgtggaggttactcacgtggacatggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACATTACCAGTTGCCTGCTGAAG 284 351 2113 Antisense
AAGTGTCTTCCCATAATATTCGCAA 273 159 2138 Antisense
AATGCGGGCATTGTGAGGCGACTGG 301 165 2204 Antisense
TGGAGAACAAGGTCATCACCCTCTA 415 549 2226 Antisense
TCATCACCCTCTAATTTGTCCAAAA 282 619 2238 Antisense
GAGTCTTCGCAGAAGCCGATCGATC 344 399 2296 Antisense
GATACTGTACCGGATGTTCCCGATC 579 423 2347 Antisense
ATCAGTCACATAAATCTCGTCACAA 451 29 2390 Antisense
TCTACTGGTAGCTATCGTGGACGAT 179 615 2479 Antisense
AGTGGAGGCAGTGGACGTGGTGGTG 281 59 2515 Antisense
TGGAGGTTACTCACGTGGACATGGT 465 547 2544 Antisense
Sequence Source Affymetrix Proprietary Database