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[186749_at]

Description Y71F9AL.1 /REP_DB=WormBase Gene ID /WP=CE25550 /TR=Q9N4H1 /GB=AAF36002.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 63      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 93.6
(3)
A 		79
(-2.3)
A 		83.8
(-0.5)
A 		77
(-3)
A 		17
B 85.4
(3)
A 		43.7
(-3)
A 		72.6
(1.2)
A 		67.8
(0.5)
A 		42
C 97.5
(1.6)
A 		87.5
(-0.8)
A 		103.4
(3)
A 		78.2
(-3)
A 		25
avr 92.2
(3) 		70.1
(-3) 		86.6
(1.5) 		74.3
(-1.9) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 48.3
(-3)
A 		72.4
(-1.8)
A 		110.9
(0.2)
A 		80.5
(-1.4)
A 		144.3
(1.9)
A 		165.5
(3)
A 		117
B 102.9
(-2.8)
A 		116.1
(-2)
A 		99.1
(-3)
A 		109.8
(-2.3)
A 		141.1
(-0.4)
A 		197.7
(3)
P 		99
C 119.4
(-0.6)
A 		124
(0.1)
A 		106.1
(-2.4)
A 		101.5
(-3)
A 		124.5
(0.1)
A 		145.6
(3)
P 		44
avr 90.2
(-3) 		104.2
(-1.9) 		105.4
(-1.9) 		97.3
(-2.5) 		136.6
(0.5) 		169.6
(3) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 34.1
(3)
A 		17.1
(-1.5)
A 		21.9
(-0.2)
A 		11.3
(-3)
A 		23
B 27.2
(3)
A 		10.8
(-3)
A 		16.8
(-0.8)
A 		14.4
(-1.7)
A 		16
C 32.3
(2)
A 		25.2
(0.2)
A 		36
(3)
A 		13.2
(-3)
A 		23
avr 31.2
(3) 		17.7
(-1.5) 		24.9
(0.9) 		13
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 8.9
(-3)
A 		25.7
(-0.1)
A 		24.1
(-0.4)
A 		20.4
(-1)
A 		43.5
(3)
A 		40.3
(2.4)
A 		35
B 35.8
(-1.6)
A 		38.6
(-1.2)
A 		24.1
(-3)
A 		29.1
(-2.4)
A 		57.3
(1)
A 		73.7
(3)
P 		50
C 44.3
(2)
A 		37.8
(0.7)
A 		27
(-1.6)
A 		20.4
(-3)
A 		40.3
(1.2)
A 		48.8
(3)
P 		28
avr 29.7
(-1.8) 		34
(-0.9) 		25.1
(-2.7) 		23.3
(-3) 		47
(1.6) 		54.3
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186749_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.65
Sequence Type Exemplar sequence
Representative Public ID Y71F9AL.1 NCBI
Target Description Y71F9AL.1 /REP_DB=WormBase Gene ID /WP=CE25550 /TR=Q9N4H1 /GB=AAF36002.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058674(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058674 NCBI Caenorhabditis elegans Y71F9AL.1 (Y71F9AL.1) mRNA, complete cds. 11/11 A 
Y71F9AL.1 ENSEMBL cdna:known chromosome:CEL140:I:2925219:2928118:1 gene:Y71F9AL.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2925245-2927027(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71F9AL.1
Entrez Gene 171864 Entrez gene
SwissProt Q9N4H1 EMBL-EBI
WormBase CE29909 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256881_AT expressed protein at
CANINE_2:CFA.18232.1.S1_AT similar to putative RNA methylase cfa
CANINE_2:CFA.19307.1.S1_S_AT similar to putative RNA methylase cfa
CANINE_2:CFAAFFX.2475.1.S1_S_AT similar to putative RNA methylase cfa
DROSOPHILA_2:1627676_AT dm
DROSGENOME1:141546_AT dm
CHICKEN:GGA.9240.1.S1_AT putative RNA methylase gga
HU35KSUBA:RC_AA226919_AT chromosome 6 open reading frame 75 hs
HU35KSUBA:AA479127_AT chromosome 6 open reading frame 75 hs
HG-U133A:218877_S_AT chromosome 6 open reading frame 75 hs
HG-U133_PLUS_2:218877_S_AT chromosome 6 open reading frame 75 hs
HG-FOCUS:218877_S_AT chromosome 6 open reading frame 75 hs
HG-U133A_2:218877_S_AT chromosome 6 open reading frame 75 hs
U133_X3P:G11141892_3P_A_AT chromosome 6 open reading frame 75 hs
HG-U95C:64118_AT chromosome 6 open reading frame 75 hs
HG-U95D:86095_AT chromosome 6 open reading frame 75 hs
HU35KSUBD:RC_AA431085_AT Chromosome 6 open reading frame 75 hs
HU35KSUBD:RC_AA452156_AT Chromosome 6 open reading frame 75 hs
MG-U74BV2:106539_AT RIKEN cDNA 2410075D05 gene mm
MG-U74BV2:163254_AT RIKEN cDNA 2410075D05 gene mm
MOE430B:1430920_AT RIKEN cDNA 2410075D05 gene mm
MOUSE430_2:1430920_AT RIKEN cDNA 2410075D05 gene mm
MOE430B:1428551_AT RIKEN cDNA 2410075D05 gene mm
MOUSE430_2:1428551_AT RIKEN cDNA 2410075D05 gene mm
MU19KSUBC:TC41762_AT RIKEN cDNA 2410075D05 gene (2410075D05Rik), mRNA mm
YEAST_2:1778811_AT Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6306 DNA methylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
8170 N-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36002 Hypothetical protein Y71F9AL.1 [Caenorhabditis elegans] ref|NP_491075.2| Y71F9AL.1 [Caenorhabditis elegans] 0.0
blast CAE60585 Hypothetical protein CBG04220 [Caenorhabditis briggsae] 1.0E-176
blast AAQ10288 putative RNA methylase [Danio rerio] ref|NP_956510.2| hypothetical protein LOC393185 [Danio rerio] 4.0E-65
Sequence
Target Sequence 
>C. ELEGANS:186749_AT
taaagctttcttagacgtggtcccattcaataaagcgccaatttgcctgaaatcccccaa
aaatgagctgtttctagtggaagaatacgaaaatcccagtgatgaagctccaaagaaagt
ttatttcggaaaactaattggtgaaggacgagccgagctgaaaaccaaatataatttacg
tgatagatgttatattggaaatacaacgatggatcctgaattatcttttattcagtcaaa
tctggcaatgataagccctggcgacattgttctcgatccattcgtcggcaccggtggcct
gatccttccagctgcggagttcggagctttcgttatcggaactgaaattaattatcaaac
tgcacgagctcaaggacgatcttcccggcaaggtgtcgggcagcgaggtgaatccgagag
cattaaggcgaatttcgagcagtataagtcggagagtcaatttctaggagttttgatcgc
cgactccagcaaacacggaatttggagctgtaatgcgaaatttgacgcaattgttgcaga
tccaccttacgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAGCTTTCTTAGACGTGGTCCCA 162 641 372 Antisense
ACGTGGTCCCATTCAATAAAGCGCC 515 93 386 Antisense
GAAATCCCCCAAAAATGAGCTGTTT 61 361 420 Antisense
GATAAGCCCTGGCGACATTGTTCTC 215 423 621 Antisense
GGAGTTCGGAGCTTTCGTTATCGGA 160 513 687 Antisense
GCACGAGCTCAAGGACGATCTTCCC 609 315 733 Antisense
GATCTTCCCGGCAAGGTGTCGGGCA 72 421 749 Antisense
GGGCAGCGAGGTGAATCCGAGAGCA 690 493 769 Antisense
ATTTCTAGGAGTTTTGATCGCCGAC 75 17 831 Antisense
TCGCCGACTCCAGCAAACACGGAAT 577 597 848 Antisense
AATTGTTGCAGATCCACCTTACGGA 431 179 900 Antisense
Sequence Source Affymetrix Proprietary Database