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[186744_at]

Description Y116F11B.1 /REP_DB=WormBase Gene ID /WP=CE25720 /TR=Q9NEK7 /GB=CAB61047.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 31      4grp_high_8_8 > 44      6grp_total > 21      4grp_total > 51      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 757
1 2 3 4 range
A 375.6
(-0.7)
P 		733.6
(3)
P 		417.9
(-0.3)
P 		159.3
(-3)
A 		574
B 400
(-0.8)
P 		849.8
(3)
P 		360.6
(-1.1)
P 		135
(-3)
A 		715
C 397.3
(-1.1)
P 		892.3
(3)
P 		416.7
(-0.9)
P 		162.4
(-3)
P 		730
avr 391
(-0.9) 		825.2
(3) 		398.4
(-0.8) 		152.2
(-3) 		673

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 662
1 2 3 4 5 6 range
A 379.7
(0)
P 		450.7
(0.9)
P 		631.7
(3)
P 		213.9
(-1.9)
P 		256.6
(-1.4)
P 		123.7
(-3)
A 		508
B 369.8
(-1)
P 		689
(2.1)
P 		785.2
(3)
P 		255
(-2.1)
P 		245.6
(-2.1)
P 		155.4
(-3)
P 		630
C 406.3
(0.3)
P 		641.7
(3)
P 		470.3
(1)
P 		286.1
(-1.1)
P 		230.6
(-1.8)
P 		125.1
(-3)
P 		517
avr 385.3
(0) 		593.8
(2.6) 		629.1
(3) 		251.7
(-1.6) 		244.3
(-1.7) 		134.7
(-3) 		494
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 233
1 2 3 4 range
A 136.8
(2)
P 		159.2
(3)
P 		109.2
(0.8)
P 		23.3
(-3)
A 		136
B 127.6
(0.3)
P 		209.9
(3)
P 		83.6
(-1.2)
P 		28.8
(-3)
A 		181
C 131.5
(-0.3)
P 		256.6
(3)
P 		145.2
(0.1)
P 		27.4
(-3)
P 		229
avr 132
(0.5) 		208.6
(3) 		112.7
(-0.2) 		26.5
(-3) 		182

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 199
1 2 3 4 5 6 range
A 70
(-1.2)
P 		160.3
(3)
P 		137.6
(2)
P 		54.3
(-1.9)
P 		77.4
(-0.8)
P 		30.1
(-3)
A 		130
B 128.7
(-0.5)
P 		229.3
(3)
P 		190.7
(1.6)
P 		67.5
(-2.7)
P 		99.8
(-1.5)
P 		57.9
(-3)
P 		171
C 150.6
(1.2)
P 		195.8
(3)
P 		119.7
(0)
P 		57.5
(-2.4)
P 		74.6
(-1.7)
P 		41.9
(-3)
P 		154
avr 116.4
(-0.1) 		195.1
(3) 		149.3
(1.2) 		59.8
(-2.3) 		83.9
(-1.4) 		43.3
(-3) 		152
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186744_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13472
Sequence Type Exemplar sequence
Representative Public ID Y116F11B.1 NCBI
Target Description Y116F11B.1 /REP_DB=WormBase Gene ID /WP=CE25720 /TR=Q9NEK7 /GB=CAB61047.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075439(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075439 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-28) (daf-28) mRNA, complete cds. 11/11 None
GENEFINDER00000031774 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19813442:19814144:1 11/11 None
Y116F11B.1 ENSEMBL cdna:known chromosome:CEL140:V:19813442:19814144:1 gene:Y116F11B.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19813441-19814144(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol daf-28
Ensembl Y116F11B.1
Entrez Gene 191014 Entrez gene
SwissProt Q9NEK7 EMBL-EBI
WormBase CE25720 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
5576 extracellular region inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
5158 insulin receptor binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61047 Hypothetical protein Y116F11B.1 [Caenorhabditis elegans] ref|NP_507840.1| abnormal DAuer Formation family member (daf-28) [Caenorhabditis elegans] 3.0E-40
blast AAC46744 Insulin related protein 4 [Caenorhabditis elegans] ref|NP_495196.1| INSulin related family member (ins-4) [Caenorhabditis elegans] sp|Q09626|ILB1_CAEEL Probable insulin-like peptide beta-type 1 precursor 2.0E-10
blast CAE59234 Hypothetical protein CBG02555 [Caenorhabditis briggsae] 2.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 8.3E-42
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507840.1 1 5-27
Y116F11B.1 1 5-27
GENEFINDER00000031774 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:186744_AT
caacttcaaggccgagggcccactaagccgtgcagtccgtgttccaggtgtggccgtgag
ggcctgtggtcgtcgcctcgttccgtatgtgtggagtgtctgtggagacgcctgtgaacc
acaagaaggaattgacatcgcaacacaatgctgcacctatcaatgcactgccgagtatat
acaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACTTCAAGGCCGAGGGCCCACTA 202 183 93 Antisense
TTCAAGGCCGAGGGCCCACTAAGCC 662 413 97 Antisense
CCAGGTGTGGCCGTGAGGGCCTGTG 462 263 136 Antisense
CGCCTCGTTCCGTATGTGTGGAGTG 627 257 166 Antisense
GTATGTGTGGAGTGTCTGTGGAGAC 49 457 177 Antisense
TGTCTGTGGAGACGCCTGTGAACCA 442 555 189 Antisense
GAGACGCCTGTGAACCACAAGAAGG 531 393 197 Antisense
AGAAGGAATTGACATCGCAACACAA 365 73 216 Antisense
GACATCGCAACACAATGCTGCACCT 397 369 226 Antisense
ATGCTGCACCTATCAATGCACTGCC 123 43 240 Antisense
TCAATGCACTGCCGAGTATATACAA 440 627 252 Antisense
Sequence Source Affymetrix Proprietary Database