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[186734_s_at]

Description Y105C5B.28 /REP_DB=WormBase Gene ID /WP=CE24078 /TR=Q9U307 /GB=CAB60321.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 47      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 37      6grp_total > 58      4grp_total > 10      6grp_highest_sub > Cu      6grp_cgc > 07      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8488
1 2 3 4 range
A 5110.8
(-3)
P 		6401.4
(-2.1)
P 		5905.6
(-2.4)
P 		13541.1
(3)
P 		8430
B 5441.5
(-3)
P 		5436.8
(-3)
P 		5688.2
(-2.8)
P 		12727.1
(3)
P 		7290
C 5052.9
(-3)
P 		5942.9
(-2.2)
P 		5454.2
(-2.6)
P 		11922.1
(3)
P 		6869
avr 5201.7
(-3) 		5927
(-2.4) 		5682.7
(-2.6) 		12730.1
(3) 		7528

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2455
1 2 3 4 5 6 range
A 4988.8
(0.2)
P 		4510.8
(-1.1)
P 		4669.7
(-0.7)
P 		4134.2
(-2.2)
P 		3829.4
(-3)
P 		6034.1
(3)
P 		2205
B 5114.4
(1.3)
P 		3931.3
(-2.2)
P 		4735.4
(0.2)
P 		3710.3
(-2.9)
P 		3662.7
(-3)
P 		5667.2
(3)
P 		2005
C 5307.5
(3)
P 		4128.2
(-1.1)
P 		5205
(2.6)
P 		3578.7
(-3)
P 		4633.6
(0.7)
P 		4546.4
(0.4)
P 		1729
avr 5136.9
(2) 		4190.1
(-1.6) 		4870
(1) 		3807.7
(-3) 		4041.9
(-2.1) 		5415.9
(3) 		1608
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1394
1 2 3 4 range
A 1860.8
(1.8)
P 		1389.6
(-3)
P 		1543.8
(-1.4)
P 		1982.2
(3)
P 		593
B 1735.2
(-1.2)
P 		1342.8
(-2.9)
P 		1318.1
(-3)
P 		2712.2
(3)
P 		1394
C 1672.6
(-3)
P 		1708.6
(-2.4)
P 		1900.9
(1.1)
P 		2010.2
(3)
P 		338
avr 1756.2
(-0.8) 		1480.3
(-3) 		1587.6
(-2.1) 		2234.9
(3) 		755

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1394
1 2 3 4 5 6 range
A 919.6
(-3)
P 		1604.3
(3)
P 		1017
(-2.1)
P 		1048.5
(-1.9)
P 		1155.5
(-0.9)
P 		1469.1
(1.8)
P 		685
B 1779.6
(1.2)
P 		1308.6
(-1.3)
P 		1149.9
(-2.1)
P 		982.7
(-3)
P 		1488.1
(-0.3)
P 		2113.3
(3)
P 		1131
C 1967.3
(3)
P 		1259.6
(-0.4)
P 		1324.7
(-0.1)
P 		719.7
(-3)
P 		1499.4
(0.7)
P 		1521.9
(0.9)
P 		1248
avr 1555.5
(1.9) 		1390.8
(0.6) 		1163.9
(-1.1) 		917
(-3) 		1381
(0.5) 		1701.4
(3) 		784
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186734_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9461
Sequence Type Exemplar sequence
Representative Public ID Y105C5B.28 NCBI
Target Description Y105C5B.28 /REP_DB=WormBase Gene ID /WP=CE24078 /TR=Q9U307 /GB=CAB60321.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070516(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070516 NCBI Caenorhabditis elegans GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-3) (gln-3) mRNA, complete cds. 11/11 None
GENEFINDER00000018428 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16167858:16170580:-1 11/11 None
Y105C5B.28 ENSEMBL cdna:known chromosome:CEL140:IV:16167746:16170786:-1 gene:Y105C5B.28 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018415 6/11 Cross Hyb Matching Probes None
SNAP00000018416 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16167863-16170645(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gln-3
Ensembl Y105C5B.28
Entrez Gene 178452 Entrez gene
SwissProt Q9U307 EMBL-EBI
WormBase CE24078 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258160_AT glutamine synthetase (GS1) at
CANINE:1582715_AT glutamate-ammonia ligase (glutamine synthetase) cfa
CANINE_2:CFA.1407.1.S2_AT glutamate-ammonia ligase (glutamine synthetase) cfa
CANINE_2:CFAAFFX.20286.1.S1_S_AT glutamate-ammonia ligase (glutamine synthetase) cfa
DROSOPHILA_2:1630653_A_AT Glutamine synthetase 2 dm
DROSGENOME1:143181_AT Glutamine synthetase 2 dm
CHICKEN:GGA.2464.1.S1_AT glutamate-ammonia ligase (glutamine synthetase) gga
CHICKEN:GGA.2464.1.S2_AT glutamate-ammonia ligase (glutamine synthetase) gga
HG-U133_PLUS_2:215001_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133A_2:215001_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-FOCUS:215001_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133A:215001_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133_PLUS_2:217202_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133A_2:217202_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133A:217202_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133_PLUS_2:200648_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133A:200648_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U133A_2:200648_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
HUGENEFL:X59834_AT glutamate-ammonia ligase (glutamine synthetase) hs
U133_X3P:G4504026_3P_A_AT glutamate-ammonia ligase (glutamine synthetase) hs
U133_X3P:HS.247984.0.S1_3P_S_AT glutamate-ammonia ligase (glutamine synthetase) hs
U133_X3P:HS.170171.1.A1_3P_A_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U95AV2:40522_AT glutamate-ammonia ligase (glutamine synthetase) hs
HG-U95D:74914_AT glutamate-ammonia ligase (glutamine synthetase) hs
HU35KSUBA:W26531_AT Glutamate-ammonia ligase (glutamine synthetase) hs
MOE430A:1426235_A_AT glutamate-ammonia ligase (glutamine synthetase) mm
MOUSE430_2:1426235_A_AT glutamate-ammonia ligase (glutamine synthetase) mm
MOUSE430A_2:1426235_A_AT glutamate-ammonia ligase (glutamine synthetase) mm
MU11KSUBB:X16314_S_AT glutamate-ammonia ligase (glutamine synthetase) mm
MG-U74AV2:99498_AT glutamate-ammonia ligase (glutamine synthetase) mm
MG-U74AV2:94852_AT glutamate-ammonia ligase (glutamine synthetase) mm
MG-U74BV2:117284_AT glutamate-ammonia ligase (glutamine synthetase) mm
MG-U74BV2:117285_G_AT glutamate-ammonia ligase (glutamine synthetase) mm
MG-U74AV2:161826_R_AT glutamate-ammonia ligase (glutamine synthetase) mm
MOE430A:1426236_A_AT glutamate-ammonia ligase (glutamine synthetase) mm
MOUSE430A_2:1426236_A_AT glutamate-ammonia ligase (glutamine synthetase) mm
MOUSE430_2:1426236_A_AT glutamate-ammonia ligase (glutamine synthetase) mm
MU19KSUBA:TC23020_S_AT Glutamine synthetase (Glul) mm
MU19KSUBB:TC30374_S_AT Glutamine synthetase (Glul) mm
YEAST_2:1774425_AT Glutamine synthetase (GS), synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9399 nitrogen fixation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4356 glutamate-ammonia ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60321 Hypothetical protein Y105C5B.28 [Caenorhabditis elegans] ref|NP_502917.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-3) [Caenorhabditis elegans] 0.0
blast CAE68163 Hypothetical protein CBG13820 [Caenorhabditis briggsae] 0.0
blast Q04831 Glutamine synthetase (Glutamate--ammonia ligase) gb|AAA02583.1| glutamine synthetase 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 6.9E-39
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 5.1E-110
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 6.9E-39
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 5.1E-110
Sequence
Target Sequence 
>C. ELEGANS:186734_S_AT
tctacgccggaatccagatctcgggaaccaacgccgaagtgatgcccggacaatgggagt
tccaggtcgggccatgcgaaggaatccagatgggagatcagttgtgggtggctcgctact
tgctgcaacgtgtcgccgaggagtttggcgtgatcgcctcgttcgattgtaagccgatca
agggagactggaacggagccggatgtcacaccaacttctccactgataagatgagaaatc
caggaggaattgacgagatcatgtccgccatcaacaagctctcccttgtccacccacaac
acattgcctactacgatccacacggtggaaaagacaacgagcgtcgtttgaccggactcc
acgagaccgcatcgatcgacaagttctcctacggagtcgcatcccgtgcctcatccattc
gtattccacgctccaccgacgatgatggatacggatacttcgaagatcgccgtccatcat
ccaactgtgacccgtacaccgtcaccggagccctcgtcagaactgtgtgtcttgagggag
ccgagcgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTACGCCGGAATCCAGATCTCGGG 499 611 578 Antisense
GAACCAACGCCGAAGTGATGCCCGG 520 343 602 Antisense
GGAGATCAGTTGTGGGTGGCTCGCT 148 519 670 Antisense
AGGAGTTTGGCGTGATCGCCTCGTT 616 55 716 Antisense
TCGCCTCGTTCGATTGTAAGCCGAT 248 599 731 Antisense
CCACAACACATTGCCTACTACGATC 540 275 871 Antisense
CCACGAGACCGCATCGATCGACAAG 343 273 936 Antisense
GATCGACAAGTTCTCCTACGGAGTC 174 415 951 Antisense
CGGATACTTCGAAGATCGCCGTCCA 598 243 1029 Antisense
AGCCCTCGTCAGAACTGTGTGTCTT 386 83 1086 Antisense
GTGTGTCTTGAGGGAGCCGAGCGTA 608 485 1102 Antisense
Sequence Source Affymetrix Proprietary Database