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[186731_at]

Description ZK1127.12 /REP_DB=WormBase Gene ID /WP=CE07650 /TR=Q23401 /GB=AAB93434.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 92      6grp_total > 66      4grp_total > 21      6grp_highest_sub > Da      6grp_high1_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 290.4
(-1.2)
P 		248.7
(-3)
P 		294.9
(-1)
P 		384.3
(3)
P 		136
B 321.4
(0.9)
P 		340.1
(1.7)
P 		233.1
(-3)
P 		368.5
(3)
P 		135
C 351.6
(3)
P 		320.4
(0.6)
P 		272.5
(-3)
P 		314
(0.1)
P 		79
avr 321.1
(0.7) 		303.1
(-0.5) 		266.8
(-3) 		355.6
(3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 381
1 2 3 4 5 6 range
A 222.9
(-3)
P 		291.9
(0.8)
P 		332.1
(3)
P 		262.5
(-0.8)
P 		241.2
(-2)
P 		330.5
(2.9)
P 		109
B 229.8
(-3)
P 		244.5
(-2.7)
P 		360.8
(-0.1)
P 		308.3
(-1.3)
P 		272.4
(-2.1)
P 		499.1
(3)
P 		269
C 201.6
(-2.6)
P 		265.9
(-1.6)
P 		179
(-3)
P 		252.7
(-1.8)
P 		197.6
(-2.7)
P 		560.3
(3)
P 		381
avr 218.1
(-3) 		267.4
(-1.8) 		290.6
(-1.2) 		274.5
(-1.6) 		237.1
(-2.5) 		463.3
(3) 		245
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 105.7
(3)
P 		54
(-3)
P 		77.1
(-0.3)
P 		56.3
(-2.7)
P 		52
B 102.5
(3)
P 		84
(0.7)
P 		54
(-3)
P 		78.5
(0)
P 		49
C 116.4
(3)
P 		92.1
(0.7)
P 		95
(1)
P 		52.9
(-3)
P 		64
avr 108.2
(3) 		76.7
(-1.1) 		75.4
(-1.3) 		62.6
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 41.1
(-3)
P 		103.8
(3)
P 		72.3
(-0)
P 		66.6
(-0.6)
P 		72.8
(0)
P 		80.5
(0.8)
P 		63
B 80
(-3)
P 		81.4
(-2.9)
P 		87.6
(-2.6)
P 		81.6
(-2.9)
P 		110.7
(-1.3)
P 		186.1
(3)
P 		106
C 74.7
(-1.8)
P 		81.1
(-1.5)
P 		45.6
(-3)
P 		50.8
(-2.8)
P 		63.9
(-2.2)
P 		187.6
(3)
P 		142
avr 65.3
(-3) 		88.8
(-1.4) 		68.5
(-2.8) 		66.3
(-2.9) 		82.5
(-1.8) 		151.4
(3) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186731_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5393
Sequence Type Exemplar sequence
Representative Public ID ZK1127.12 NCBI
Target Description ZK1127.12 /REP_DB=WormBase Gene ID /WP=CE07650 /TR=Q23401 /GB=AAB93434.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063045(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063045 NCBI Caenorhabditis elegans ZK1127.12 (ZK1127.12) mRNA, complete cds. 11/11 None
ZK1127.12 ENSEMBL cdna:known chromosome:CEL140:II:7052029:7053717:1 gene:ZK1127.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033590 5/11 Cross Hyb Matching Probes None
SNAP00000033591 6/11 Cross Hyb Matching Probes None
GENEFINDER00000033601 6/11 Cross Hyb Matching Probes None
GENEFINDER00000033605 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7052286-7053663(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK1127.12
Entrez Gene 174152 Entrez gene
SwissProt Q23401 EMBL-EBI
WormBase CE33491 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB93434 Hypothetical protein ZK1127.12 [Caenorhabditis elegans] ref|NP_495446.2| ZK1127.12 [Caenorhabditis elegans] 1.0E-138
blast CAE66019 Hypothetical protein CBG11212 [Caenorhabditis briggsae] 9.0E-55
Sequence
Target Sequence 
>C. ELEGANS:186731_AT
tagacggctttcaaattatttcgctagaaatagatgaagaagagaatgggcatctcttag
ttcgactgaagtcgtttaaatataacgaaacattaacaatttctttgaatgtcgaggatt
tccgaggttttcctcggtttctccgctgctcgaatccgtcaagaatggcaagcttcgatt
gtgaaaaatggattgccgaagataaattgggaaccaatctgcgaagaatttatggcgaat
tggaggcagcccccctttcggaaaagcaatttgacaataaatcgaaagattcattggagg
aatatacaggaccggctcagatttctgcaaactttattcgtgagaaaatcgatggagaat
cgatggaaagaggaatgttcagaggaagaggattggaaggtgctgagtggaatgcaccag
aaggttatgcgatacatgttctgaaggagagaaaaggtccgaaaggaacgattctcgata
tagagtctggacctgattcgattcgtgtttggcaatgggaccgaacgtgcggcgaaaaca
atgctgatcgcacggcgattgcacgagcctcgaatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGACGGCTTTCAAATTATTTCGCT 145 653 425 Antisense
AATGGGCATCTCTTAGTTCGACTGA 323 163 469 Antisense
TGTCGAGGATTTCCGAGGTTTTCCT 284 557 534 Antisense
GCTGCTCGAATCCGTCAAGAATGGC 339 297 569 Antisense
TTATGGCGAATTGGAGGCAGCCCCC 300 671 654 Antisense
TACAGGACCGGCTCAGATTTCTGCA 98 645 729 Antisense
GATTTCTGCAAACTTTATTCGTGAG 693 427 744 Antisense
AGTCTGGACCTGATTCGATTCGTGT 306 61 908 Antisense
GGCAATGGGACCGAACGTGCGGCGA 658 531 935 Antisense
AAAACAATGCTGATCGCACGGCGAT 343 129 959 Antisense
CGGCGATTGCACGAGCCTCGAATTA 140 241 977 Antisense
Sequence Source Affymetrix Proprietary Database