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[186725_at]

Description Y54E10A.5 /REP_DB=WormBase Gene ID /WP=CE24449 /TR=Q9N3F1 /GB=AAF60762.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 73      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 65      6grp_total > 74      4grp_total > 63      6grp_high1_sub > 03_04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 604
1 2 3 4 range
A 1376.8
(2.9)
P 		785.9
(-3)
P 		1389.5
(3)
P 		1020.1
(-0.7)
P 		604
B 1175.1
(-1.4)
P 		1126.6
(-2.8)
P 		1325.8
(3)
P 		1120.5
(-3)
P 		205
C 1365.7
(3)
P 		1215.1
(-0.2)
P 		1192.2
(-0.7)
P 		1085.4
(-3)
P 		280
avr 1305.9
(3) 		1042.5
(-3) 		1302.5
(2.9) 		1075.3
(-2.3) 		263

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 552
1 2 3 4 5 6 range
A 1035.2
(-3)
P 		1076.9
(-2.4)
P 		1477
(3)
P 		1429.4
(2.4)
P 		1240.3
(-0.2)
P 		1387.7
(1.8)
P 		442
B 1227.5
(-0.8)
P 		1033.4
(-3)
P 		1554.5
(3)
P 		1231.1
(-0.7)
P 		1306.2
(0.1)
P 		1332.2
(0.4)
P 		521
C 1050.7
(-3)
P 		1061.6
(-2.9)
P 		1232.4
(-1)
P 		1585.8
(3)
P 		1346.2
(0.3)
P 		1285.6
(-0.4)
P 		535
avr 1104.5
(-2.2) 		1057.3
(-3) 		1421.3
(3) 		1415.4
(2.9) 		1297.6
(1) 		1335.2
(1.6) 		364
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 501.3
(3)
P 		170.6
(-2.6)
P 		363.2
(0.6)
P 		149.3
(-3)
P 		352
B 374.7
(3)
P 		278.2
(-1.3)
P 		307.2
(0)
P 		238.8
(-3)
P 		136
C 452.1
(3)
P 		349.4
(0.7)
P 		415.5
(2.2)
P 		183
(-3)
P 		269
avr 442.7
(3) 		266.1
(-1.2) 		362
(1.1) 		190.4
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 340
1 2 3 4 5 6 range
A 190.8
(-3)
P 		383
(3)
P 		321.7
(1.1)
P 		362.5
(2.4)
P 		374.3
(2.7)
P 		337.9
(1.6)
P 		192
B 427.1
(-0)
P 		344
(-2.5)
P 		377.5
(-1.5)
P 		326.1
(-3)
P 		530.7
(3)
P 		496.8
(2)
P 		205
C 389.5
(0.7)
P 		323.9
(-2.5)
P 		313.7
(-3)
P 		318.9
(-2.7)
P 		435.6
(3)
P 		430.4
(2.7)
P 		122
avr 335.8
(-3) 		350.3
(-2.2) 		337.6
(-2.9) 		335.8
(-3) 		446.9
(3) 		421.7
(1.6) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186725_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.301
Sequence Type Exemplar sequence
Representative Public ID Y54E10A.5 NCBI
Target Description Y54E10A.5 /REP_DB=WormBase Gene ID /WP=CE24449 /TR=Q9N3F1 /GB=AAF60762.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058715(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058715 NCBI Caenorhabditis elegans Y54E10A.5 (Y54E10A.5) mRNA, complete cds. 11/11 None
GENEFINDER00000033840 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3163693:3171124:1 10/11 None
SNAP00000033828 ENSEMBL cdna:SNAP chromosome:CEL140:I:3168135:3171124:1 11/11 None
Y54E10A.5.2 ENSEMBL cdna:known chromosome:CEL140:I:3168161:3171124:1 gene:Y54E10A.5 11/11 None
Y54E10A.5.1 ENSEMBL cdna:known chromosome:CEL140:I:3168194:3171170:1 gene:Y54E10A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3168134-3171124(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E10A.5
Entrez Gene 171891 Entrez gene
SwissProt Q9N3F1 EMBL-EBI
WormBase CE34408 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1588863_AT similar to Dynactin subunit 6 (Dynactin subunit p27) (WS-3 protein) cfa
CANINE_2:CFA.3404.1.S1_AT similar to Dynactin subunit 6 (Dynactin subunit p27) (WS-3 protein) cfa
CANINE_2:CFAAFFX.10737.1.S1_S_AT similar to Dynactin subunit 6 (Dynactin subunit p27) (WS-3 protein) cfa
CANINE_2:CFAAFFX.10743.1.S1_AT similar to Dynactin subunit 6 (Dynactin subunit p27) (WS-3 protein) cfa
DROSOPHILA_2:1626156_AT dm
DROSGENOME1:146480_AT dm
CHICKEN:GGA.5816.1.S1_AT similar to Dynactin 6 (WS-3 protein) gga
CHICKEN:GGA.5816.2.S1_A_AT similar to Dynactin 6 (WS-3 protein) gga
HG-U133A:203261_AT dynactin 6 hs
HG-U133A_2:203261_AT dynactin 6 hs
HG-U133_PLUS_2:203261_AT dynactin 6 hs
HG-FOCUS:203261_AT dynactin 6 hs
HUGENEFL:D84145_AT dynactin 6 hs
U133_X3P:G5730115_3P_AT dynactin 6 hs
HG-U95AV2:34781_AT dynactin 6 hs
MU11KSUBA:AA217493_S_AT dynactin 6 mm
MG-U74AV2:160327_AT dynactin 6 mm
MOE430A:1416499_A_AT dynactin 6 mm
MOUSE430A_2:1416499_A_AT dynactin 6 mm
MOUSE430_2:1416499_A_AT dynactin 6 mm
MOE430A:1448368_AT dynactin 6 mm
MOUSE430A_2:1448368_AT dynactin 6 mm
MOUSE430_2:1448368_AT dynactin 6 mm
MU19KSUBA:TC14483_S_AT Dynactin 6, mRNA (cDNA clone MGC:36027 IMAGE:4486579) mm
MU19KSUBB:TC26028_S_AT Dynactin 6, mRNA (cDNA clone MGC:36027 IMAGE:4486579) mm
RG-U34C:RC_AI175025_AT dynactin 6 (predicted) rn
RAE230A:1371533_AT dynactin 6 (predicted) rn
RAT230_2:1371533_AT dynactin 6 (predicted) rn
RAE230B:1396392_AT Dynactin 6 (predicted) rn
RAT230_2:1396392_AT Dynactin 6 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60763 Hypothetical protein Y54E10A.6 [Caenorhabditis elegans] ref|NP_491115.1| Y54E10A.6 [Caenorhabditis elegans] 0.0
blast CAE60545 Hypothetical protein CBG04172 [Caenorhabditis briggsae] 0.0
blast AAF60762 Hypothetical protein Y54E10A.5 [Caenorhabditis elegans] ref|NP_491116.2| Y54E10A.5 [Caenorhabditis elegans] 3.0E-92
blast AAF60762 Hypothetical protein Y54E10A.5 [Caenorhabditis elegans] ref|NP_491116.2| Y54E10A.5 [Caenorhabditis elegans] 4.0E-92
blast AAF60762 Hypothetical protein Y54E10A.5 [Caenorhabditis elegans] ref|NP_491116.2| Y54E10A.5 [Caenorhabditis elegans] 1.0E-88
blast CAE60544 Hypothetical protein CBG04171 [Caenorhabditis briggsae] 1.0E-81
blast CAE60544 Hypothetical protein CBG04171 [Caenorhabditis briggsae] 4.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.17
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0035
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.026
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 2.7E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.085
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.086
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 10.0
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.16
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.086
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 10.0
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.16
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.086
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 10.0
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.16
Sequence
Target Sequence 
>C. ELEGANS:186725_AT
gaaactagcacagtatccatcgccagtagtgcaatcgtctgcgtcgaagcggatataaaa
ggagaagttataattaaagaaggctgcgtcgtccatccatttgtagtgttcgacgccaca
aaaggcccaatttatgttggagaaaataatatttttgaagaatacgcagttattcggaat
aacagcgacggtcaaccaatgataatcggtgactggaacatctttcaagtccactcgaaa
tccagtgcaaaatacgttggaagccggaatgtgattggtgtgcatgcggtgctcgaagac
ggttgctccgtttcagatgactgtagcgttggagccaagtgtacagtgttttcccatcaa
aatctggaaccttctgtgtctgtttatgcagccacgaatttgagtagaacgacgaaaacg
ccgaatatgacatcgccccaccaaattgaattcctgcgtaaaatcctaccatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACTAGCACAGTATCCATCGCCA 481 355 97 Antisense
ATCCATCGCCAGTAGTGCAATCGTC 154 37 111 Antisense
GCAATCGTCTGCGTCGAAGCGGATA 618 321 127 Antisense
TTAAAGAAGGCTGCGTCGTCCATCC 83 677 170 Antisense
GTCGTCCATCCATTTGTAGTGTTCG 316 473 184 Antisense
CATGCGGTGCTCGAAGACGGTTGCT 248 211 379 Antisense
GACGGTTGCTCCGTTTCAGATGACT 694 375 394 Antisense
GGAACCTTCTGTGTCTGTTTATGCA 249 531 462 Antisense
AAAACGCCGAATATGACATCGCCCC 494 127 511 Antisense
GCCCCACCAAATTGAATTCCTGCGT 564 285 531 Antisense
ATTCCTGCGTAAAATCCTACCATCA 590 7 546 Antisense
Sequence Source Affymetrix Proprietary Database