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[186717_at]

Description F42A8.3 /REP_DB=WormBase Gene ID /WP=CE01580 /TR=SW:Q09322 /GB=CAA87781.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 96      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 95      6grp_total > 86      4grp_total > 60      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 424
1 2 3 4 range
A 470.7
(1.8)
P 		449.3
(1.1)
P 		507.9
(3)
P 		325.5
(-3)
P 		182
B 537.1
(3)
P 		493.7
(2.2)
P 		528.4
(2.8)
P 		216.3
(-3)
P 		321
C 449.6
(-0.6)
P 		640.2
(3)
P 		514.8
(0.6)
P 		323.6
(-3)
P 		317
avr 485.8
(1.9) 		527.7
(3) 		517
(2.7) 		288.5
(-3) 		239

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 755
1 2 3 4 5 6 range
A 447.2
(-3)
P 		450.5
(-3)
P 		710.1
(0.4)
P 		667.5
(-0.2)
P 		845.7
(2.1)
P 		915.6
(3)
P 		468
B 448.2
(-3)
P 		601.3
(-1.5)
P 		643.2
(-1.1)
P 		639.7
(-1.2)
P 		850.7
(0.9)
P 		1075.1
(3)
P 		627
C 383.7
(-3)
P 		558.2
(-1.6)
P 		476.9
(-2.3)
P 		725.6
(-0.3)
P 		719.3
(-0.3)
P 		1138.8
(3)
P 		755
avr 426.4
(-3) 		536.7
(-1.9) 		610.1
(-1.2) 		677.6
(-0.6) 		805.2
(0.7) 		1043.2
(3) 		617
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 171.4
(3)
P 		97.5
(-0.6)
P 		132.8
(1.1)
P 		47.6
(-3)
P 		124
B 171.3
(3)
P 		121.9
(0.6)
P 		122.4
(0.7)
P 		46.1
(-3)
P 		125
C 148.8
(1.4)
P 		184.1
(3)
P 		179.4
(2.8)
P 		54.6
(-3)
P 		130
avr 163.8
(3) 		134.5
(1.5) 		144.9
(2) 		49.4
(-3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 319
1 2 3 4 5 6 range
A 82.4
(-3)
P 		160.2
(-0.3)
P 		154.6
(-0.5)
P 		169.3
(0)
P 		255.2
(3)
P 		222.9
(1.9)
P 		173
B 156
(-3)
P 		200.2
(-1.9)
P 		156.2
(-3)
P 		169.4
(-2.7)
P 		345.6
(1.6)
P 		400.9
(3)
P 		245
C 142.2
(-2.5)
P 		170.3
(-1.9)
P 		121.4
(-3)
P 		145.9
(-2.4)
P 		232.8
(-0.4)
P 		381.2
(3)
P 		260
avr 126.9
(-3) 		176.9
(-1.6) 		144.1
(-2.5) 		161.5
(-2) 		277.9
(1.4) 		335
(3) 		208
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186717_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3737
Sequence Type Exemplar sequence
Representative Public ID F42A8.3 NCBI
Target Description F42A8.3 /REP_DB=WormBase Gene ID /WP=CE01580 /TR=SW:Q09322 /GB=CAA87781.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063592(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063592 NCBI Caenorhabditis elegans F42A8.3 (F42A8.3) mRNA, complete cds. 11/11 None
SNAP00000044380 ENSEMBL cdna:SNAP chromosome:CEL140:II:9350787:9354084:1 11/11 None
GENEFINDER00000044385 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9352912:9354084:1 11/11 None
F42A8.3 ENSEMBL cdna:known chromosome:CEL140:II:9352912:9354175:1 gene:F42A8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9352722-9354081(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F42A8.3
Entrez Gene 174483 Entrez gene
WormBase CE35875 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA87781 Hypothetical protein F42A8.3 [Caenorhabditis elegans] ref|NP_495993.2| F42A8.3 [Caenorhabditis elegans] 0.0
blast Q09322 Hypothetical protein F42A8.3 in chromosome II precursor 0.0
blast CAA87781 Hypothetical protein F42A8.3 [Caenorhabditis elegans] ref|NP_495993.2| F42A8.3 [Caenorhabditis elegans] 1.0E-175
blast Q09322 Hypothetical protein F42A8.3 in chromosome II precursor 1.0E-175
blast CAE57842 Hypothetical protein CBG00871 [Caenorhabditis briggsae] 1.0E-129
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495993.2 1 245-267
F42A8.3 1 245-267
SNAP00000044380 1 242-264
GENEFINDER00000044385 1 245-267
Sequence
Target Sequence 
>C. ELEGANS:186717_AT
atccagatgtgactcctgcataacaattaaatggaattttccgcaaaacaaatcggaagt
gtcattcagtgagaggacaatgcaatttcagaacagaatgtatgaatggagaaattgtgt
tgttaattacacatctggaggtgtactggatgataatcttaccaatggaagtaaaatctg
taatctctgcaagaccacctttgacgaactctttggatattactggaaaatctacacaac
tccagatgtagatttttgtgtcgacgtggagactacgatgaacgatacaattcatttatg
ggatgatgtgtggaaatgtgctgaaaaacaagacagaaatcgtgatttatttggaattat
gatcacttttggaacattattgctgctcacagctctattttatgctgccagctacattca
gggtggcggtgaaacgaggcgtcttattcaatatgctcgtctatcagatccacatggcca
aagatcacgtcttctttcatcgggaatgtcggatgcagatcttgttagtcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCAGATGTGACTCCTGCATAACA 642 39 402 Antisense
ATCTGTAATCTCTGCAAGACCACCT 602 33 577 Antisense
AGACCACCTTTGACGAACTCTTTGG 209 71 593 Antisense
TTTGGAACATTATTGCTGCTCACAG 258 667 769 Antisense
GCTGCTCACAGCTCTATTTTATGCT 552 297 783 Antisense
TATGCTGCCAGCTACATTCAGGGTG 425 657 802 Antisense
AGGGTGGCGGTGAAACGAGGCGTCT 503 57 821 Antisense
TATGCTCGTCTATCAGATCCACATG 541 657 853 Antisense
TCCACATGGCCAAAGATCACGTCTT 618 587 870 Antisense
TCACGTCTTCTTTCATCGGGAATGT 428 625 886 Antisense
GTCGGATGCAGATCTTGTTAGTCGT 210 473 909 Antisense
Sequence Source Affymetrix Proprietary Database