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[186715_at]

Description F46C5.2 /REP_DB=WormBase Gene ID /WP=CE03344 /TR=SW:P52881 /GB=CAA91050.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 31      4grp_high_8_8 > 20      6grp_highest_nonorm > 51      6grp_total > 13      4grp_total > 20      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 399
1 2 3 4 range
A 599
(-0.6)
P 		468
(-2.6)
P 		439
(-3)
P 		837.7
(3)
P 		399
B 576.5
(0.6)
P 		651.2
(3)
P 		465.7
(-3)
P 		630.8
(2.3)
P 		186
C 601.5
(-3)
P 		700.4
(-0.4)
P 		611.2
(-2.7)
P 		829.9
(3)
P 		228
avr 592.3
(-1) 		606.5
(-0.7) 		505.3
(-3) 		766.1
(3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 501
1 2 3 4 5 6 range
A 705.4
(3)
P 		655.9
(2.3)
P 		668.1
(2.5)
P 		496.1
(0.2)
P 		368.6
(-1.5)
P 		260.9
(-3)
P 		445
B 740
(3)
P 		661.3
(1.9)
P 		507.8
(-0.1)
P 		566.8
(0.7)
P 		352.5
(-2.2)
P 		296.9
(-3)
P 		443
C 487.3
(-0.8)
P 		761.5
(3)
P 		464.8
(-1.2)
P 		503.4
(-0.6)
P 		333.8
(-3)
P 		383.8
(-2.3)
P 		428
avr 644.2
(2.2) 		692.9
(3) 		546.9
(0.7) 		522.1
(0.3) 		351.6
(-2.4) 		313.9
(-3) 		379
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 218.1
(3)
P 		101.6
(-3)
P 		114.8
(-2.3)
P 		122.6
(-1.9)
P 		117
B 183.9
(3)
P 		160.8
(1.2)
P 		107.9
(-3)
P 		134.4
(-0.9)
P 		76
C 199.1
(1.9)
P 		201.4
(2)
P 		213
(3)
P 		139.9
(-3)
P 		73
avr 200.4
(3) 		154.6
(-1) 		145.2
(-1.9) 		132.3
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 130
(-0.7)
P 		233.3
(3)
P 		145.5
(-0.1)
P 		125.8
(-0.8)
P 		111.2
(-1.3)
P 		63.5
(-3)
P 		170
B 257.5
(3)
P 		220.1
(1.5)
P 		123.3
(-2.5)
P 		150.1
(-1.4)
P 		143.2
(-1.7)
P 		110.7
(-3)
P 		147
C 180.6
(0.6)
P 		232.4
(3)
P 		118.3
(-2.2)
P 		101.2
(-3)
P 		108
(-2.7)
P 		128.5
(-1.8)
P 		131
avr 189.4
(1.2) 		228.6
(3) 		129
(-1.7) 		125.7
(-1.8) 		120.8
(-2.1) 		100.9
(-3) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186715_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2981
Sequence Type Exemplar sequence
Representative Public ID F46C5.2 NCBI
Target Description F46C5.2 /REP_DB=WormBase Gene ID /WP=CE03344 /TR=SW:P52881 /GB=CAA91050.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063480(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063480 NCBI Caenorhabditis elegans F46C5.2 (F46C5.2) mRNA, complete cds. 11/11 None
SNAP00000022656 ENSEMBL cdna:SNAP chromosome:CEL140:II:8828186:8829132:-1 11/11 None
GENEFINDER00000022662 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8828425:8829729:-1 10/11 None
F46C5.2 ENSEMBL cdna:known chromosome:CEL140:II:8828066:8829201:-1 gene:F46C5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8828182-8829030(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F46C5.2
Entrez Gene 185844 Entrez gene
SwissProt P52881 EMBL-EBI
WormBase CE03344 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91050 Hypothetical protein F46C5.2 [Caenorhabditis elegans] ref|NP_495881.1| F46C5.2 [Caenorhabditis elegans] sp|P52881|YAF2_CAEEL Hypothetical protein F46C5.2 in chromosome II 2.0E-91
blast CAA91050 Hypothetical protein F46C5.2 [Caenorhabditis elegans] ref|NP_495881.1| F46C5.2 [Caenorhabditis elegans] sp|P52881|YAF2_CAEEL Hypothetical protein F46C5.2 in chromosome II 3.0E-91
blast CAE59746 Hypothetical protein CBG03190 [Caenorhabditis briggsae] 1.0E-87
blast CAE59746 Hypothetical protein CBG03190 [Caenorhabditis briggsae] 2.0E-87
blast CAA91050 Hypothetical protein F46C5.2 [Caenorhabditis elegans] ref|NP_495881.1| F46C5.2 [Caenorhabditis elegans] sp|P52881|YAF2_CAEEL Hypothetical protein F46C5.2 in chromosome II 3.0E-81
blast CAE59746 Hypothetical protein CBG03190 [Caenorhabditis briggsae] 3.0E-78
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495881.1 1 158-180
F46C5.2 1 158-180
SNAP00000022656 2 15-32,190-212
Sequence
Target Sequence 
>C. ELEGANS:186715_AT
gtgttcagtgtgttcaactttggcgttcagcttcagcaaaagaagggaaggtgtgcacga
gtggagctaccacttgcaaaggaaatgcttgttttatgagacaatgcaagcactgtccag
tatatcaatacatgtcaggatgtgtcaacttctctccgtggcaattggcagatttagaaa
tgaaccgtcgtacatcagaacttcgaatgagacgggttggtgcagttctgttatgtgaag
atacttttaatcagacaacgtgtgtctgtaatagacgagacaaatgcaatgatattcact
ctcgactaccatttgccacgtatgctgagggccttttccgaggtgtcgtcaattttgaca
ctattattgcggctatcgatccaagatatttggaagtgatgtctggttatcatttccggt
ttctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTCAGTGTGTTCAACTTTGGCG 172 485 62 Antisense
AACTTTGGCGTTCAGCTTCAGCAAA 192 139 77 Antisense
AGGTGTGCACGAGTGGAGCTACCAC 108 55 110 Antisense
TCAGGATGTGTCAACTTCTCTCCGT 395 623 196 Antisense
TTCTCTCCGTGGCAATTGGCAGATT 534 691 211 Antisense
TGAACCGTCGTACATCAGAACTTCG 695 571 242 Antisense
GAGACGGGTTGGTGCAGTTCTGTTA 599 393 270 Antisense
CAATGATATTCACTCTCGACTACCA 472 181 348 Antisense
TGCCACGTATGCTGAGGGCCTTTTC 232 587 375 Antisense
TTGACACTATTATTGCGGCTATCGA 285 705 416 Antisense
GTCTGGTTATCATTTCCGGTTTCTT 676 471 462 Antisense
Sequence Source Affymetrix Proprietary Database