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[186712_s_at]

Description T28A11.17 /REP_DB=WormBase Gene ID /WP=CE14311 /TR=P91515 /GB=AAC48126.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 61      4grp_total > 01      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 1.4
(-3)
A 		5.5
(-2)
A 		1.8
(-2.9)
A 		27.2
(3)
A 		26
B 2.5
(-2.8)
A 		1.9
(-3)
A 		2.5
(-2.8)
A 		22.5
(3)
A 		21
C 3.5
(-2.7)
A 		2.4
(-3)
A 		8.6
(-1.5)
A 		27.6
(3)
A 		25
avr 2.5
(-3) 		3.3
(-2.8) 		4.3
(-2.5) 		25.8
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 0.8
(-3)
A 		0.9
(-3)
A 		26.7
(3)
A 		2.6
(-2.6)
A 		4.2
(-2.2)
A 		1.1
(-2.9)
A 		26
B 1.1
(-2.8)
A 		3.1
(-1.3)
A 		4.8
(0)
A 		8.7
(3)
A 		1.9
(-2.2)
A 		0.9
(-3)
A 		8
C 1
(-2.9)
A 		3.1
(-2.2)
A 		2.6
(-2.3)
A 		17.2
(3)
A 		2.2
(-2.5)
A 		0.8
(-3)
A 		16
avr 1
(-2.9) 		2.4
(-2.1) 		11.4
(3) 		9.5
(1.9) 		2.8
(-1.9) 		0.9
(-3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 0.5
(-3)
A 		1.2
(-1.8)
A 		0.5
(-3)
A 		4
(3)
A 		4
B 0.8
(-2.6)
A 		0.5
(-3)
A 		0.6
(-2.9)
A 		4.8
(3)
A 		4
C 1.2
(-2.3)
A 		0.7
(-3)
A 		3
(0.5)
A 		4.7
(3)
A 		4
avr 0.8
(-3) 		0.8
(-3) 		1.4
(-2) 		4.5
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 0.2
(-3)
A 		0.3
(-2.9)
A 		5.8
(3)
A 		0.7
(-2.5)
A 		1.3
(-1.8)
A 		0.3
(-2.9)
A 		6
B 0.4
(-2.7)
A 		1
(-0.9)
A 		1.2
(-0.3)
A 		2.3
(3)
A 		0.8
(-1.5)
A 		0.3
(-3)
A 		2
C 0.4
(-2.8)
A 		0.9
(-1.9)
A 		0.7
(-2.3)
A 		3.5
(3)
A 		0.7
(-2.3)
A 		0.3
(-3)
A 		3
avr 0.3
(-3) 		0.7
(-2) 		2.6
(3) 		2.2
(2) 		0.9
(-1.4) 		0.3
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186712_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14830
Sequence Type Exemplar sequence
Representative Public ID T28A11.17 NCBI
Target Description T28A11.17 /REP_DB=WormBase Gene ID /WP=CE14311 /TR=P91515 /GB=AAC48126.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071497(11), NM_071520(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071497 NCBI Caenorhabditis elegans T28A11.17 (T28A11.17) mRNA, complete cds. 11/11 None
NM_071520 NCBI Caenorhabditis elegans C17B7.8 (C17B7.8) mRNA, complete cds. 11/11 None
SNAP00000020303 ENSEMBL cdna:SNAP chromosome:CEL140:V:3320294:3323708:-1 11/11 None
GENEFINDER00000020322 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3319819:3324080:-1 11/11 None
SNAP00000028210 ENSEMBL cdna:SNAP chromosome:CEL140:V:3263453:3266081:-1 11/11 None
GENEFINDER00000028228 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3263453:3266081:-1 11/11 None
T28A11.17 ENSEMBL cdna:known chromosome:CEL140:V:3263453:3266081:-1 gene:T28A11.17 11/11 None
C17B7.8 ENSEMBL cdna:known chromosome:CEL140:V:3320574:3323938:-1 gene:C17B7.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3263452-3266081(-) 100.0 100.0
chrV:3320664-3324080(-) 92.79 92.79
Public Domain and Genome References
Gene Title

Ensembl T28A11.17
C17B7.8
Entrez Gene 182708 Entrez gene
189022 Entrez gene
SwissProt O45151 EMBL-EBI
P91515 EMBL-EBI
WormBase CE16856 Wormbase
CE35789 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503898 T28A11.17 [Caenorhabditis elegans] gb|AAC48126.2| Hypothetical protein T28A11.17 [Caenorhabditis elegans] 0.0
blast AAC04402 Hypothetical protein C17B7.8 [Caenorhabditis elegans] ref|NP_503921.1| C17B7.8 [Caenorhabditis elegans] 0.0
blast AAF59425 Hypothetical protein Y19D10B.6 [Caenorhabditis elegans] ref|NP_503636.1| Y19D10B.6 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.9E-86
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.0E-88
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.0E-88
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.0E-88
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.9E-86
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.9E-86
Sequence
Target Sequence 
>C. ELEGANS:186712_S_AT
cactttgtctatccattcggaatacggttactgccttctaatgttacaaaagcagcaaac
aagtgtacacaagatgaagtcaagatgatgggtgactctgatgccgtgaagccaatagat
ggatggttcagtgaaaacatcactcacgaagatcttccaaatttattgggattaaggatg
gttatgaagatggtcgcaaggaaatcggcgaatgaagagcagctgaaaaatgcgcttgaa
acaattttgagcggattatgcatccaaggcaaaccaaaaaactcaattctaccaaatcat
catccctttgaaatatccatcaacacagccgttcgccaatatccattgttcagttcactc
tatggatgtcgccttggtgatcgaatgtttgctgaaccggaaaagttttgcaatccactg
ggaggcgacgtgaacttggaggactataaaatcaagaatgatactgaagatgtcggtgga
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTTTGTCTATCCATTCGGAATAC 428 195 961 Antisense
GTCTATCCATTCGGAATACGGTTAC 481 469 967 Antisense
AATACGGTTACTGCCTTCTAATGTT 387 171 981 Antisense
GGTTACTGCCTTCTAATGTTACAAA 286 507 986 Antisense
GGTTCAGTGAAAACATCACTCACGA 418 507 1085 Antisense
AACATCACTCACGAAGATCTTCCAA 154 139 1096 Antisense
GATGGTCGCAAGGAAATCGGCGAAT 200 407 1149 Antisense
TCCACTGGGAGGCGACGTGAACTTG 332 279 1374 Antisense
GGGAGGCGACGTGAACTTGGAGGAC 7 495 1380 Antisense
GGCGACGTGAACTTGGAGGACTATA 102 541 1384 Antisense
AATGATACTGAAGATGTCGGTGGAT 282 163 1417 Antisense
Sequence Source Affymetrix Proprietary Database