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[186687_at]

Description T28F3.3 /REP_DB=WormBase Gene ID /WP=CE19807 /TR=SW:Q9XUC4 /GB=CAB05297.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 32      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 29.9
(-3)
A 		67.7
(0.2)
A 		101.2
(3)
A 		60.5
(-0.4)
P 		71
B 71.8
(0)
A 		44.3
(-3)
A 		82.7
(1.2)
A 		99.1
(3)
M 		55
C 51.8
(-3)
A 		68.5
(-1.2)
A 		107.6
(3)
P 		88.5
(0.9)
A 		56
avr 51.2
(-3) 		60.2
(-1.8) 		97.2
(3) 		82.7
(1.1) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 423
1 2 3 4 5 6 range
A 67.9
(-2.8)
A 		75.3
(-2.7)
P 		55
(-3)
A 		82.8
(-2.5)
P 		277.5
(0.7)
P 		416.5
(3)
P 		362
B 39.6
(-3)
M 		78.1
(-2.5)
A 		73.8
(-2.5)
P 		131
(-1.7)
P 		321.3
(1)
P 		462.9
(3)
P 		423
C 61
(-3)
M 		102.1
(-2.4)
P 		85.1
(-2.6)
A 		105.5
(-2.3)
P 		247.5
(-0.2)
P 		461.3
(3)
P 		400
avr 56.2
(-3) 		85.2
(-2.6) 		71.3
(-2.8) 		106.4
(-2.2) 		282.1
(0.5) 		446.9
(3) 		391
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 10.9
(-2.3)
A 		14.7
(-1)
A 		26.4
(3)
A 		8.9
(-3)
P 		18
B 22.9
(3)
A 		11
(-3)
A 		19.2
(1.1)
A 		21.1
(2.1)
M 		12
C 17.2
(-2.4)
A 		19.7
(-1.7)
A 		37.5
(3)
P 		14.9
(-3)
A 		23
avr 17
(-2.1) 		15.1
(-3) 		27.7
(3) 		15
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 12.5
(-3)
A 		26.8
(-2)
P 		12
(-3)
A 		21
(-2.4)
P 		83.7
(1.8)
P 		101.4
(3)
P 		89
B 13.8
(-3)
M 		26
(-2.5)
A 		17.9
(-2.8)
P 		34.7
(-2.2)
P 		130.5
(1.4)
P 		172.6
(3)
P 		159
C 22.6
(-2.9)
M 		31.2
(-2.5)
P 		21.7
(-3)
A 		21.2
(-3)
P 		80.1
(-0.3)
P 		154.4
(3)
P 		133
avr 16.3
(-3) 		28
(-2.4) 		17.2
(-3) 		25.6
(-2.6) 		98.1
(0.9) 		142.8
(3) 		127
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186687_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10168
Sequence Type Exemplar sequence
Representative Public ID T28F3.3 NCBI
Target Description T28F3.3 /REP_DB=WormBase Gene ID /WP=CE19807 /TR=SW:Q9XUC4 /GB=CAB05297.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070669(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070669 NCBI Caenorhabditis elegans T28F3.3 (T28F3.3) mRNA, complete cds. 11/11 None
SNAP00000032158 ENSEMBL cdna:SNAP chromosome:CEL140:IV:17286032:17287407:-1 11/11 None
GENEFINDER00000032168 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:17285930:17287407:-1 11/11 None
T28F3.3 ENSEMBL cdna:known chromosome:CEL140:IV:17285957:17287407:-1 gene:T28F3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17285935-17287413(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28F3.3
Entrez Gene 178509 Entrez gene
SwissProt Q9XUC4 EMBL-EBI
WormBase CE36329 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30001 metal ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
46873 metal ion transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05297 Hypothetical protein T28F3.3 [Caenorhabditis elegans] ref|NP_503070.2| T28F3.3 [Caenorhabditis elegans] sp|Q9XUC4|YGJK_CAEEL Hypothetical protein T28F3.3 in chromosome IV 1.0E-164
blast CAB05297 Hypothetical protein T28F3.3 [Caenorhabditis elegans] ref|NP_503070.2| T28F3.3 [Caenorhabditis elegans] sp|Q9XUC4|YGJK_CAEEL Hypothetical protein T28F3.3 in chromosome IV 1.0E-162
blast CAE57421 Hypothetical protein CBG00379 [Caenorhabditis briggsae] 1.0E-136
blast CAE63324 Hypothetical protein CBG07716 [Caenorhabditis briggsae] 4.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 4.0E-116
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 3.0E-116
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503070.2 7 83-105,117-139,169-191,253-275,298-320,333-355,370-392
T28F3.3 7 83-105,117-139,169-191,253-275,298-320,333-355,370-392
SNAP00000032158 7 83-105,117-139,169-191,253-275,298-320,333-355,370-392
GENEFINDER00000032168 7 83-105,117-139,169-191,253-275,298-320,333-355,370-388
Sequence
Target Sequence 
>C. ELEGANS:186687_AT
aagccagtgcctacttgaacttggttgccgatttcgtgcataatgtcaccgatggattgg
cgattggcgcttcattttcagctggaaatactcttggatggatcacaacactgactgtgc
ttcttcacgagctacctcacgaagtcggagattttgcgattctcgtgcaatctggattct
ctaaatatcaggcaatccgtttgcaagccgttactgctctcggagcaatcaccggatgcg
ttttctcccttcttgtctcaaatcctggatccctgaacaacgatgcagataccagtgcta
ttatgccttttacagctggcggattcatctacatcgcgacggtttccgttgtcccggagc
ttctcgagtccggagatcacaacaatctgtcgaaagtggcaaaaatggcgcagagcttgg
tgcatgttttggccatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCCAGTGCCTACTTGAACTTGGT 370 149 713 Antisense
GAACTTGGTTGCCGATTTCGTGCAT 31 351 729 Antisense
TCACCGATGGATTGGCGATTGGCGC 594 625 758 Antisense
ATTGGCGCTTCATTTTCAGCTGGAA 530 3 775 Antisense
CAACACTGACTGTGCTTCTTCACGA 403 189 818 Antisense
TCACGAGCTACCTCACGAAGTCGGA 320 627 837 Antisense
TTTGCAAGCCGTTACTGCTCTCGGA 417 667 912 Antisense
AGCAATCACCGGATGCGTTTTCTCC 141 75 936 Antisense
GCTGGCGGATTCATCTACATCGCGA 222 295 1027 Antisense
TACATCGCGACGGTTTCCGTTGTCC 668 641 1042 Antisense
GAGCTTGGTGCATGTTTTGGCCATC 77 389 1125 Antisense
Sequence Source Affymetrix Proprietary Database