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[186676_s_at]

Description F35H10.10 /REP_DB=WormBase Gene ID /WP=CE24945 /TR=Q20073 /GB=AAA81683.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_high_8_8 > 52      6grp_highest_nonorm > 44      6grp_total > 82      4grp_total > 59      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2107
1 2 3 4 range
A 1467.6
(0.7)
P 		2244.7
(3)
P 		1877.7
(1.9)
P 		179.4
(-3)
A 		2065
B 1215.5
(0.8)
P 		1828.7
(3)
P 		1771.9
(2.8)
P 		162
(-3)
P 		1667
C 1288.7
(0.7)
P 		1845.6
(2.5)
P 		2002.5
(3)
P 		137.3
(-3)
P 		1865
avr 1323.9
(0.9) 		1973
(3) 		1884
(2.7) 		159.6
(-3) 		1813

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1559
1 2 3 4 5 6 range
A 1414.5
(-1.1)
P 		2309.2
(2.8)
P 		1567.9
(-0.4)
P 		2355.2
(3)
P 		2204.6
(2.3)
P 		969.2
(-3)
P 		1386
B 1482.5
(-1)
P 		2199.5
(2)
P 		1458.2
(-1.1)
P 		2430.4
(3)
P 		2098.9
(1.6)
P 		1013.6
(-3)
P 		1417
C 1238.5
(-1.5)
P 		2314.1
(3)
P 		1876.6
(1.2)
P 		2309
(3)
P 		2315
(3)
P 		871
(-3)
P 		1444
avr 1378.5
(-1.2) 		2274.3
(2.6) 		1634.2
(-0.1) 		2364.9
(3) 		2206.2
(2.3) 		951.3
(-3) 		1414
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 675
1 2 3 4 range
A 534.4
(3)
P 		487.3
(2.4)
P 		490.9
(2.5)
P 		26.3
(-3)
A 		508
B 387.6
(2.1)
P 		451.6
(3)
P 		410.6
(2.4)
P 		34.5
(-3)
P 		417
C 426.6
(0.6)
P 		530.6
(1.5)
P 		697.9
(3)
P 		23.2
(-3)
P 		675
avr 449.5
(2) 		489.8
(2.5) 		533.1
(3) 		28
(-3) 		505

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 617
1 2 3 4 5 6 range
A 260.8
(-2.7)
P 		821.3
(3)
P 		341.5
(-1.9)
P 		597.3
(0.7)
P 		665.2
(1.4)
P 		236
(-3)
P 		585
B 515.8
(-1.1)
P 		732.1
(1.5)
P 		354.1
(-3)
P 		643.7
(0.5)
P 		852.7
(3)
P 		378
(-2.7)
P 		499
C 459.1
(-0.8)
P 		706.1
(2.4)
P 		477.6
(-0.6)
P 		464.3
(-0.7)
P 		749.1
(3)
P 		291.6
(-3)
P 		458
avr 411.9
(-1.5) 		753.2
(3) 		391.1
(-1.8) 		568.4
(0.5) 		755.7
(3) 		301.9
(-3) 		454
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186676_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9833
Sequence Type Exemplar sequence
Representative Public ID F35H10.10 NCBI
Target Description F35H10.10 /REP_DB=WormBase Gene ID /WP=CE24945 /TR=Q20073 /GB=AAA81683.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068999(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068999 NCBI Caenorhabditis elegans F35H10.10 (F35H10.10) mRNA, complete cds. 11/11 A 
SNAP00000039427 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8307639:8312525:-1 11/11 None
GENEFINDER00000039436 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8307639:8312902:-1 11/11 None
F35H10.10 ENSEMBL cdna:known chromosome:CEL140:IV:8307301:8312921:-1 gene:F35H10.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8307644-8312908(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H10.10
Entrez Gene 177627 Entrez gene
SwissProt Q20073 EMBL-EBI
WormBase CE24945 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8067 metabotropic glutamate, GABA-B-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81683 Hypothetical protein F35H10.10 [Caenorhabditis elegans] ref|NP_501400.1| F35H10.10 [Caenorhabditis elegans] gb|AAB51198.1| CELF35-1 [Caenorhabditis elegans] 0.0
blast CAE58455 Hypothetical protein CBG01593 [Caenorhabditis briggsae] 0.0
blast XP_692887 PREDICTED: similar to glutamate receptor, metabotropic 2 precursor [Danio rerio] 1.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR000337 EMBL-EBI G-protein coupled receptor family 3, metabotropic glutamate receptor-like 2.0E-83
Pfam IPR000337 EMBL-EBI G-protein coupled receptor family 3, metabotropic glutamate receptor-like 2.0E-83
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501400.1 7 1025-1047,1060-1080,1090-1107,1128-1150,1175-1197,1210-1232,1242-1264
F35H10.10 7 1025-1047,1060-1080,1090-1107,1128-1150,1175-1197,1210-1232,1242-1264
SNAP00000039427 7 977-999,1012-1032,1042-1059,1080-1102,1127-1149,1162-1184,1194-1216
GENEFINDER00000039436 7 1025-1047,1060-1080,1090-1107,1128-1150,1175-1197,1210-1232,1242-1264
Sequence
Target Sequence 
>C. ELEGANS:186676_S_AT
tcttcacttgggtcgcatggatcacactttacttggttctcgatcatgagttcagggaca
ctgttattgtcgttgaactggttgcctgtgcaacaattcttcttgggttcttgttcggac
ctaaaatctatattcttctatcctatgagccagttgtagttgcatttaagagagatccat
tcccaaatcatactgatctattcgaaaaagacgatgatcttccatcacaacgtgcggtgt
ctccagcttcatcaacatcttcttcttcaaatcgttcatcatctggatcctcatacacat
catcttcaaaacgacctgttaacgctgttggaccacttccattaaacaagaatgttgagg
atcagtcaccaatattccatacggttatgaggaagaagaccaaagttcggcgatccaata
gcgaacatgataccattatgaacgttggtattccgatgacatcggtccctatcattcgaa
gtccatcggctcgtgacgaaaaggttcatcgagtggccatatcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCACTTGGGTCGCATGGATCAC 76 617 3668 Antisense
GGATCACACTTTACTTGGTTCTCGA 553 511 3686 Antisense
TGTCGTTGAACTGGTTGCCTGTGCA 557 557 3735 Antisense
TCTTCTTGGGTTCTTGTTCGGACCT 203 615 3765 Antisense
TATTCTTCTATCCTATGAGCCAGTT 233 663 3798 Antisense
ATCTTCCATCACAACGTGCGGTGTC 405 31 3884 Antisense
ACATCTTCTTCTTCAAATCGTTCAT 170 107 3922 Antisense
GTTCATCATCTGGATCCTCATACAC 71 445 3941 Antisense
TGTTAACGCTGTTGGACCACTTCCA 45 561 3984 Antisense
ATCGGTCCCTATCATTCGAAGTCCA 394 21 4128 Antisense
AGGTTCATCGAGTGGCCATATCGCC 568 57 4169 Antisense
Sequence Source Affymetrix Proprietary Database