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[186661_at]

Description F46C5.4 /REP_DB=WormBase Gene ID /WP=CE03346 /TR=SW:P52882 /GB=CAA91046.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 14      4grp_high_8_8 > 08      6grp_total > 14      4grp_total > 23      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 437.9
(3)
P 		148
(-3)
A 		226.7
(-1.4)
M 		391.5
(2)
P 		290
B 259.6
(1.8)
P 		229.3
(0.8)
P 		115.7
(-3)
A 		296.5
(3)
P 		181
C 426.8
(3)
P 		264.8
(-2.6)
M 		252.5
(-3)
P 		270.9
(-2.4)
P 		174
avr 374.8
(3) 		214
(-2.5) 		198.3
(-3) 		319.6
(1.1) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 292
1 2 3 4 5 6 range
A 275
(1)
P 		360.6
(3)
P 		278
(1.1)
P 		243.3
(0.2)
P 		150.8
(-1.9)
A 		105
(-3)
A 		256
B 396.9
(3)
P 		233.8
(-1.1)
P 		224.5
(-1.4)
P 		180.7
(-2.5)
A 		252.7
(-0.7)
P 		160.2
(-3)
P 		237
C 254.1
(2.5)
P 		262.3
(3)
P 		227
(0.6)
P 		211.7
(-0.4)
P 		178.8
(-2.6)
A 		172.8
(-3)
P 		90
avr 308.7
(3) 		285.6
(2.1) 		243.2
(0.6) 		211.9
(-0.6) 		194.1
(-1.2) 		146
(-3) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 159.5
(3)
P 		32.1
(-3)
A 		59.3
(-1.7)
M 		57.3
(-1.8)
P 		127
B 82.8
(3)
P 		56.6
(0.2)
P 		26.8
(-3)
A 		63.2
(0.9)
P 		56
C 141.3
(3)
P 		76.1
(-1.1)
M 		88
(-0.3)
P 		45.7
(-3)
P 		96
avr 127.9
(3) 		54.9
(-3) 		58
(-2.7) 		55.4
(-3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 50.7
(-1.5)
P 		128.3
(3)
P 		60.5
(-1)
P 		61.7
(-0.9)
P 		45.5
(-1.8)
A 		25.6
(-3)
A 		103
B 138.1
(3)
P 		77.8
(-1)
P 		54.5
(-2.6)
P 		47.9
(-3)
A 		102.7
(0.6)
P 		59.7
(-2.2)
P 		90
C 94.2
(3)
P 		80
(1.3)
P 		57.8
(-1.2)
P 		42.6
(-3)
P 		57.9
(-1.2)
A 		57.8
(-1.2)
P 		52
avr 94.3
(2.9) 		95.4
(3) 		57.6
(-1.8) 		50.7
(-2.6) 		68.7
(-0.4) 		47.7
(-3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186661_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3122
Sequence Type Exemplar sequence
Representative Public ID F46C5.4 NCBI
Target Description F46C5.4 /REP_DB=WormBase Gene ID /WP=CE03346 /TR=SW:P52882 /GB=CAA91046.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063481(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063481 NCBI Caenorhabditis elegans F46C5.4 (F46C5.4) mRNA, complete cds. 11/11 None
GENEFINDER00000022659 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8836356:8839153:1 9/11 None
F46C5.4 ENSEMBL cdna:known chromosome:CEL140:II:8836356:8838697:1 gene:F46C5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8836352-8838694(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F46C5.4
Entrez Gene 185845 Entrez gene
SwissProt P52882 EMBL-EBI
WormBase CE03346 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91046 Hypothetical protein F46C5.4 [Caenorhabditis elegans] ref|NP_495882.1| F46C5.4 [Caenorhabditis elegans] sp|P52882|YAF4_CAEEL Hypothetical protein F46C5.4 in chromosome II 2.0E-99
blast CAA91046 Hypothetical protein F46C5.4 [Caenorhabditis elegans] ref|NP_495882.1| F46C5.4 [Caenorhabditis elegans] sp|P52882|YAF4_CAEEL Hypothetical protein F46C5.4 in chromosome II 5.0E-73
blast CAE59747 Hypothetical protein CBG03192 [Caenorhabditis briggsae] 9.0E-66
blast CAE59747 Hypothetical protein CBG03192 [Caenorhabditis briggsae] 1.0E-60
Sequence
Target Sequence 
>C. ELEGANS:186661_AT
cgatttcgtcgatacattggcttgttattcgtgtattgctttgaattacagacaaaacgt
gctctcccgcaacgacgctttgtctcctccgcagaatcgcgagaatctcactgcgttgtt
tgatgtcttgtctaaggtatccattttcatctttgccttttcatttcaacatgatcagtt
tcagaacaacattagccacgtggaagtgtcctcgagttgtgctgacgtcactttaacaac
ccaaccgtcttttctgaatactccgattgcaatatgtgatatgaatgataaatgcgttaa
aatggacttttattattctggtaaagtatattctatgtcaatcactaattcaaaacattt
aaaattttcaggagagaaggtagtgcttcgcaattgtctttcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATTTCGTCGATACATTGGCTTGT 595 247 72 Antisense
GGCTTGTTATTCGTGTATTGCTTTG 508 537 90 Antisense
ATTACAGACAAAACGTGCTCTCCCG 453 11 116 Antisense
GTCTCCTCCGCAGAATCGCGAGAAT 497 469 153 Antisense
GCGAGAATCTCACTGCGTTGTTTGA 485 289 170 Antisense
AGGTATCCATTTTCATCTTTGCCTT 659 57 206 Antisense
ATCTTTGCCTTTTCATTTCAACATG 394 31 220 Antisense
AAGTGTCCTCGAGTTGTGCTGACGT 430 159 275 Antisense
TGCTGACGTCACTTTAACAACCCAA 200 583 291 Antisense
CCAACCGTCTTTTCTGAATACTCCG 354 275 312 Antisense
TAGTGCTTCGCAATTGTCTTTCAAA 96 655 452 Antisense
Sequence Source Affymetrix Proprietary Database