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[186655_at]

Description T28A11.16 /REP_DB=WormBase Gene ID /WP=CE14309 /TR=P91512 /GB=AAC48125.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 52      4grp_high_8_8 > 30      6grp_total > 62      4grp_total > 33      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 75.4
(-3)
A 		150.1
(-0.1)
P 		181.4
(1.2)
P 		228.1
(3)
P 		153
B 78
(-3)
P 		160.5
(2.6)
P 		153.7
(2.2)
P 		166
(3)
P 		88
C 95.1
(-3)
P 		159.6
(1.1)
P 		139.5
(-0.2)
P 		189.2
(3)
P 		94
avr 82.8
(-3) 		156.7
(1) 		158.2
(1.1) 		194.4
(3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 256
1 2 3 4 5 6 range
A 83.3
(-2.4)
A 		193.6
(0.5)
P 		288.2
(3)
P 		216.1
(1.1)
P 		190.7
(0.4)
P 		59.5
(-3)
P 		229
B 75
(-1.8)
P 		194.7
(1.5)
P 		250.7
(3)
P 		185.3
(1.2)
P 		129.7
(-0.3)
P 		31.8
(-3)
A 		219
C 83.5
(-2.4)
P 		256.2
(3)
P 		243.7
(2.6)
P 		211.5
(1.6)
P 		120
(-1.3)
P 		65
(-3)
P 		191
avr 80.6
(-2.2) 		214.8
(1.7) 		260.9
(3) 		204.3
(1.4) 		146.8
(-0.3) 		52.1
(-3) 		209
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 27.4
(-3)
A 		32.6
(-1.4)
P 		47.4
(3)
P 		33.4
(-1.2)
P 		20
B 24.9
(-3)
P 		39.6
(3)
P 		35.6
(1.4)
P 		35.4
(1.3)
P 		15
C 31.5
(-3)
P 		45.9
(2.1)
P 		48.6
(3)
P 		31.9
(-2.9)
P 		17
avr 27.9
(-3) 		39.4
(1.3) 		43.9
(3) 		33.6
(-0.9) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 15.4
(-2.9)
A 		68.9
(3)
P 		62.8
(2.3)
P 		54.8
(1.4)
P 		57.6
(1.8)
P 		14.5
(-3)
P 		54
B 26.1
(-1.4)
P 		64.8
(3)
P 		60.9
(2.6)
P 		49.1
(1.2)
P 		52.7
(1.6)
P 		11.8
(-3)
A 		53
C 30.9
(-2)
P 		78.2
(3)
P 		62
(1.3)
P 		42.5
(-0.8)
P 		38.8
(-1.2)
P 		21.8
(-3)
P 		56
avr 24.1
(-2.1) 		70.6
(3) 		61.9
(2) 		48.8
(0.6) 		49.7
(0.7) 		16
(-3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186655_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15436
Sequence Type Exemplar sequence
Representative Public ID T28A11.16 NCBI
Target Description T28A11.16 /REP_DB=WormBase Gene ID /WP=CE14309 /TR=P91512 /GB=AAC48125.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071494(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071494 NCBI Caenorhabditis elegans T28A11.16 (T28A11.16) mRNA, complete cds. 11/11 None
SNAP00000028208 ENSEMBL cdna:SNAP chromosome:CEL140:V:3258347:3259042:-1 11/11 None
GENEFINDER00000028238 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3258347:3259042:-1 11/11 None
T28A11.16 ENSEMBL cdna:known chromosome:CEL140:V:3258347:3259042:-1 gene:T28A11.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3258346-3259042(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28A11.16
Entrez Gene 189021 Entrez gene
SwissProt P91512 EMBL-EBI
WormBase CE14309 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503895 T28A11.16 [Caenorhabditis elegans] gb|AAC48125.1| Hypothetical protein T28A11.16 [Caenorhabditis elegans] 1.0E-109
blast AAC04398 Hypothetical protein C17B7.4 [Caenorhabditis elegans] ref|NP_503926.1| C17B7.4 [Caenorhabditis elegans] 2.0E-60
blast NP_503901 T28A11.5 [Caenorhabditis elegans] gb|AAC48116.2| Hypothetical protein T28A11.5 [Caenorhabditis elegans] 2.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 3.6E-81
Sequence
Target Sequence 
>C. ELEGANS:186655_AT
gtgttccatcaaacttgctgtgcttggagcagtcctgcttgcattcgcatgtagttccaa
ccaggctccaagtaatgaaacaagtattatcgagtctgaacttggaacaaattgcaccac
ggttacctttaaaaatttcctcggttgcctttatcgattcggagattttatggcaaagat
gtactttctggatgtaacgaagcagaagagtttagagaaatttcataaatcgtgcaattc
catggccgattgtattgagaatcttgagtgtcataaagataaaaaccttaccgactccaa
aacaatgagaaatgcttgtgagactctgaaattcttcgtcaccgatttttttacttgttt
ggaaaaaattggagaattgaaaccggaaccccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTCCATCAAACTTGCTGTGCTT 323 485 18 Antisense
CTGTGCTTGGAGCAGTCCTGCTTGC 604 237 35 Antisense
AGTCCTGCTTGCATTCGCATGTAGT 378 61 48 Antisense
ATTCGCATGTAGTTCCAACCAGGCT 246 9 60 Antisense
CCAACCAGGCTCCAAGTAATGAAAC 275 275 74 Antisense
GAACAAATTGCACCACGGTTACCTT 248 339 122 Antisense
CATAAATCGTGCAATTCCATGGCCG 286 211 241 Antisense
AATTCCATGGCCGATTGTATTGAGA 151 179 253 Antisense
ACCTTACCGACTCCAAAACAATGAG 672 85 302 Antisense
TGAAATTCTTCGTCACCGATTTTTT 483 571 344 Antisense
TGGAGAATTGAAACCGGAACCCCAG 670 547 387 Antisense
Sequence Source Affymetrix Proprietary Database