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[186652_at]

Description ZK512.8 /REP_DB=WormBase Gene ID /WP=CE00414 /TR=SW:P34646 /GB=CAA80149.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 44      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 17.7
(-3)
A 		27.3
(-1.9)
A 		71.5
(3)
A 		44.2
(-0)
A 		54
B 47.6
(0.3)
A 		73
(3)
A 		41.4
(-0.3)
A 		15.7
(-3)
A 		57
C 73.3
(2.5)
A 		79.2
(3)
A 		12.6
(-3)
A 		43.6
(-0.2)
A 		67
avr 46.2
(-0.2) 		59.8
(3) 		41.8
(-1.3) 		34.5
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 19.2
(-2.8)
A 		9
(-3)
A 		13.2
(-2.9)
A 		77.2
(-1.9)
A 		209.3
(0.3)
M 		375.5
(3)
P 		367
B 27
(-3)
A 		39
(-2.7)
A 		29.7
(-2.9)
A 		32.4
(-2.9)
A 		216.5
(2.1)
P 		249.6
(3)
P 		223
C 49.5
(-3)
A 		54
(-2.9)
A 		63.7
(-2.7)
A 		87.3
(-2.2)
A 		248.2
(1)
P 		348.8
(3)
P 		299
avr 31.9
(-3) 		34
(-3) 		35.5
(-2.9) 		65.6
(-2.3) 		224.7
(1) 		324.6
(3) 		293
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 6.4
(-2.8)
A 		5.9
(-3)
A 		18.7
(3)
A 		6.5
(-2.7)
A 		13
B 15.2
(1.9)
A 		18
(3)
A 		9.6
(-0.4)
A 		3.3
(-3)
A 		15
C 24.3
(3)
A 		22.8
(2.5)
A 		4.4
(-3)
A 		7.4
(-2.1)
A 		20
avr 15.3
(2.8) 		15.6
(3) 		10.9
(0.2) 		5.7
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 3.5
(-3)
A 		3.2
(-3)
A 		2.9
(-3)
A 		19.6
(-1.9)
A 		63.2
(1.1)
M 		91.4
(3)
P 		89
B 9.4
(-2.8)
A 		13
(-2.6)
A 		7.2
(-3)
A 		8.6
(-2.9)
A 		88
(2.6)
P 		93.1
(3)
P 		86
C 18.3
(-2.9)
A 		16.5
(-3)
A 		16.2
(-3)
A 		17.6
(-2.9)
A 		80.3
(0.8)
P 		116.7
(3)
P 		101
avr 10.4
(-2.9) 		10.9
(-2.9) 		8.8
(-3) 		15.3
(-2.6) 		77.2
(1.5) 		100.4
(3) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186652_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8004
Sequence Type Exemplar sequence
Representative Public ID ZK512.8 NCBI
Target Description ZK512.8 /REP_DB=WormBase Gene ID /WP=CE00414 /TR=SW:P34646 /GB=CAA80149.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066626(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066626 NCBI Caenorhabditis elegans ZK512.8 (ZK512.8) mRNA, complete cds. 11/11 None
SNAP00000002537 ENSEMBL cdna:SNAP chromosome:CEL140:III:9145989:9147251:1 11/11 None
GENEFINDER00000002539 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9145989:9147251:1 11/11 None
ZK512.8 ENSEMBL cdna:known chromosome:CEL140:III:9145953:9147257:1 gene:ZK512.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9145984-9147247(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK512.8
Entrez Gene 176294 Entrez gene
SwissProt P34646 EMBL-EBI
WormBase CE00414 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80149 Hypothetical protein ZK512.8 [Caenorhabditis elegans] ref|NP_499027.1| ZK512.8 [Caenorhabditis elegans] sp|P34646|YOQ8_CAEEL Hypothetical protein ZK512.8 in chromosome III 2.0E-84
blast CAB01532 Hypothetical protein ZC412.5 [Caenorhabditis elegans] ref|NP_506663.1| ZC412.5 [Caenorhabditis elegans] 1.0E-57
blast CAE62663 Hypothetical protein CBG06801 [Caenorhabditis briggsae] 5.0E-48
Sequence
Target Sequence 
>C. ELEGANS:186652_AT
atgtctcaccggaacgatagctctcgtaagcatcgtaaatcgcgttcaatcaagaatgat
caacactatccgacagtgtcttttgatggagcagagtctgtcgatattcctgcggacgaa
ggaaaaagtccagtgagcacgcacttgtcgttggcatcaaatccaactgaattcaagttc
atggatgaaattgcacttttgaagcgtggccgcatcgacaaagacgctccaaagcatccg
tacaatcgtcgtggccagcaaccaatgatgaagaaggagatttcggtgacttcggctcat
tccaacagctccttgcctgtcagaagtccagctatcatgacgttgatgggcggagttcca
gaggcagaaaaagagtacaatgttggaaagggtggacttctcaaaatttctaagatgaag
gaaggtggaaagagatacgaagtttccgccaacgagcgcttcaccgtcttccaacaaaca
gctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCTCACCGGAACGATAGCTCTC 358 47 13 Antisense
AGTCTGTCGATATTCCTGCGGACGA 400 61 107 Antisense
AAAGTCCAGTGAGCACGCACTTGTC 609 121 137 Antisense
CGCACTTGTCGTTGGCATCAAATCC 559 253 152 Antisense
GAAGCGTGGCCGCATCGACAAAGAC 7 343 213 Antisense
AAAGCATCCGTACAATCGTCGTGGC 517 123 243 Antisense
ATCGTCGTGGCCAGCAACCAATGAT 486 33 257 Antisense
AGGAGATTTCGGTGACTTCGGCTCA 83 55 287 Antisense
CTCCTTGCCTGTCAGAAGTCCAGCT 104 235 321 Antisense
ATACGAAGTTTCCGCCAACGAGCGC 206 19 447 Antisense
TCACCGTCTTCCAACAAACAGCTGG 158 625 473 Antisense
Sequence Source Affymetrix Proprietary Database