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[186648_at]

Description F44G4.1 /REP_DB=WormBase Gene ID /WP=CE28332 /TR=SW:P54073 /GB=CAA90124.4 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 54      6grp_high1_10_10 > 45      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 66      6grp_total > 36      4grp_total > 04      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6708
1 2 3 4 range
A 2172
(-2)
P 		1266.8
(-2.8)
P 		1057.6
(-3)
P 		7578.3
(3)
P 		6521
B 2384.7
(-1.6)
P 		1176.8
(-2.7)
P 		870.5
(-3)
P 		7137.5
(3)
P 		6267
C 2088.3
(-2.1)
P 		1233.1
(-3)
P 		1538.5
(-2.7)
P 		6959.8
(3)
P 		5727
avr 2215
(-2) 		1225.6
(-2.9) 		1155.5
(-3) 		7225.2
(3) 		6070

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 856
1 2 3 4 5 6 range
A 1516.8
(-1.3)
P 		2006.4
(3)
P 		1330.1
(-3)
P 		1612.3
(-0.5)
P 		1431
(-2.1)
P 		1658.6
(-0.1)
P 		676
B 1456.4
(0.9)
P 		1622.1
(3)
P 		1150
(-3)
P 		1519.5
(1.7)
P 		1521.4
(1.7)
P 		1539.3
(1.9)
P 		472
C 1778.6
(3)
P 		1718.8
(2.3)
P 		1717.6
(2.2)
P 		1638.4
(1.3)
P 		1297.6
(-3)
P 		1664.9
(1.6)
P 		481
avr 1583.9
(-0.1) 		1782.4
(3) 		1399.2
(-3) 		1590.1
(-0) 		1416.7
(-2.7) 		1620.9
(0.5) 		383
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1319
1 2 3 4 range
A 790.8
(0.7)
P 		275
(-3)
P 		276.5
(-3)
P 		1109.3
(3)
P 		834
B 760.5
(-0.5)
P 		290.6
(-2.6)
P 		201.7
(-3)
P 		1521
(3)
P 		1319
C 691.3
(-0.5)
P 		354.5
(-3)
P 		536.2
(-1.7)
P 		1173.5
(3)
P 		819
avr 747.5
(-0.2) 		306.7
(-3) 		338.1
(-2.8) 		1267.9
(3) 		961

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 434
1 2 3 4 5 6 range
A 279.6
(-3)
P 		713.6
(3)
P 		289.7
(-2.9)
P 		408.9
(-1.2)
P 		431.8
(-0.9)
P 		403.8
(-1.3)
P 		434
B 506.7
(1)
P 		539.9
(1.6)
P 		279.3
(-3)
P 		402.5
(-0.8)
P 		618.1
(3)
P 		574
(2.2)
P 		339
C 659.3
(3)
P 		524.5
(0.5)
P 		437.2
(-1)
P 		329.5
(-3)
P 		419.9
(-1.4)
P 		557.3
(1.1)
P 		330
avr 481.9
(0.4) 		592.7
(3) 		335.4
(-3) 		380.3
(-2) 		489.9
(0.6) 		511.7
(1.1) 		257
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186648_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3940
Sequence Type Exemplar sequence
Representative Public ID F44G4.1 NCBI
Target Description F44G4.1 /REP_DB=WormBase Gene ID /WP=CE28332 /TR=SW:P54073 /GB=CAA90124.4 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063517(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063517 NCBI Caenorhabditis elegans F44G4.1 (F44G4.1) mRNA, complete cds. 11/11 None
SNAP00000000988 ENSEMBL cdna:SNAP chromosome:CEL140:II:8994545:8996002:1 11/11 None
GENEFINDER00000001004 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8994545:8996808:1 11/11 None
F44G4.1 ENSEMBL cdna:known chromosome:CEL140:II:8994545:8996178:1 gene:F44G4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8993078-8995999(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44G4.1
Entrez Gene 174434 Entrez gene
SwissProt P54073 EMBL-EBI
WormBase CE28332 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13171_AT brix domain-containing protein at
ATH1-121501:255586_AT brix domain-containing protein at
CANINE:1604862_S_AT similar to RNA processing factor 1 cfa
CANINE:1592282_AT similar to RNA processing factor 1 cfa
CANINE:1590607_S_AT similar to RNA processing factor 1 cfa
CANINE_2:CFA.673.1.S1_AT similar to RNA processing factor 1 cfa
CANINE_2:CFA.673.2.S1_AT similar to RNA processing factor 1 cfa
CANINE_2:CFA.673.2.S1_S_AT similar to RNA processing factor 1 cfa
CANINE_2:CFAAFFX.31104.1.S1_S_AT similar to RNA processing factor 1 cfa
DROSOPHILA_2:1638350_AT dm
DROSGENOME1:154160_AT dm
CHICKEN:GGA.3567.1.S1_AT similar to RNA processing factor 1 gga
HG-U133_PLUS_2:218462_AT brix domain containing 5 hs
HG-U133A:218462_AT brix domain containing 5 hs
HG-U133A_2:218462_AT brix domain containing 5 hs
HU35KSUBA:W55890_AT brix domain containing 5 hs
HU35KSUBA:RC_AA279949_AT brix domain containing 5 hs
HU35KSUBC:RC_AA262912_AT brix domain containing 5 hs
U133_X3P:G13376604_3P_AT brix domain containing 5 hs
HG-U95B:44043_AT brix domain containing 5 hs
HG-U95B:48306_AT brix domain containing 5 hs
HG-U133_PLUS_2:234243_AT brix domain containing 5 hs
HG-U133B:234243_AT brix domain containing 5 hs
HG-U133_PLUS_2:234244_AT brix domain containing 5 hs
HG-U133B:234244_AT brix domain containing 5 hs
MOE430A:1428020_AT brix domain containing 5 mm
MOUSE430A_2:1428020_AT brix domain containing 5 mm
MOUSE430_2:1428020_AT brix domain containing 5 mm
MOUSE430A_2:1431784_A_AT brix domain containing 5 mm
MOE430A:1431784_A_AT brix domain containing 5 mm
MOUSE430_2:1431784_A_AT brix domain containing 5 mm
MG-U74AV2:97802_AT brix domain containing 5 mm
MG-U74CV2:169519_R_AT brix domain containing 5 mm
MOE430B:1459573_AT Brix domain containing 5 (Bxdc5), transcript variant 2, mRNA mm
MOUSE430_2:1459573_AT Brix domain containing 5 (Bxdc5), transcript variant 2, mRNA mm
MU19KSUBA:TC19666_AT Brix domain containing 5 (Bxdc5), transcript variant 2, mRNA mm
YEAST_2:1777405_AT Nucleolar protein involved in the assembly of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Sc
Protein Similarities
Method ID Description E-Value
blast CAA90124 Hypothetical protein F44G4.1 [Caenorhabditis elegans] ref|NP_495918.1| F44G4.1 [Caenorhabditis elegans] sp|P54073|YUY1_CAEEL Hypothetical protein F44G4.1 in chromosome II 1.0E-161
blast CAE59780 Hypothetical protein CBG03232 [Caenorhabditis briggsae] 1.0E-158
blast B88252 protein F44G4.1 [imported] - Caenorhabditis elegans 1.0E-158
blast CAA90124 Hypothetical protein F44G4.1 [Caenorhabditis elegans] ref|NP_495918.1| F44G4.1 [Caenorhabditis elegans] sp|P54073|YUY1_CAEEL Hypothetical protein F44G4.1 in chromosome II 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR007109 EMBL-EBI Brix domain 2.1E-83
Sequence
Target Sequence 
>C. ELEGANS:186648_AT
gagttcactgatttgcttgttgtacacgaagatagaaagaagccaaatggaattattttc
tgtcatttgcctgaaggacctactgcgtatttcaaaatcaatagtctaacattcacacag
gatttgaaggtttgttattttgataacttctttatgtattgcttaaaatcattaaaacta
ttttataaattcggtgagtcgacatctcatttcccggaagtgattctcaacaatttcaac
acacgtcttggacacaacatcgctcgaatgcttgcttgtctctttccacacgatccaaaa
ttcactggacgcagagttgtaacattccacaatcagagagattacatctttttcagacat
catagatacgaattcaaaaaggaaggttcaaaagcagcgcttctggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTTCACTGATTTGCTTGTTGTAC 36 399 1717 Antisense
GGAATTATTTTCTGTCATTTGCCTG 337 533 1765 Antisense
GCCTGAAGGACCTACTGCGTATTTC 171 283 1785 Antisense
ATAGTCTAACATTCACACAGGATTT 11 23 1817 Antisense
CGGTGAGTCGACATCTCATTTCCCG 92 243 1908 Antisense
TCATTTCCCGGAAGTGATTCTCAAC 186 619 1923 Antisense
ACACGTCTTGGACACAACATCGCTC 12 109 1957 Antisense
CAACATCGCTCGAATGCTTGCTTGT 259 189 1971 Antisense
TCTCTTTCCACACGATCCAAAATTC 615 611 1995 Antisense
GGACGCAGAGTTGTAACATTCCACA 589 523 2023 Antisense
GGTTCAAAAGCAGCGCTTCTGGAGT 333 505 2101 Antisense
Sequence Source Affymetrix Proprietary Database