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[186640_s_at]

Description Y38C1AB.4 /REP_DB=WormBase Gene ID /WP=CE25254 /TR=Q9N531 /GB=AAF60499.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 416.7
(3)
P 		356.9
(0.6)
P 		267.3
(-3)
P 		276.7
(-2.6)
P 		149
B 360.3
(3)
P 		338.3
(2.1)
P 		279.3
(-0.3)
P 		211.4
(-3)
P 		149
C 365.5
(3)
P 		357.9
(2.7)
P 		284.9
(-0.4)
P 		223.1
(-3)
P 		142
avr 380.8
(3) 		351
(1.8) 		277.2
(-1.3) 		237.1
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 335
1 2 3 4 5 6 range
A 351.2
(2.7)
P 		358.8
(3)
P 		283.6
(0.4)
P 		186.8
(-3)
P 		227.9
(-1.6)
P 		194.8
(-2.7)
P 		172
B 485
(3)
P 		314.9
(-0.2)
P 		238.5
(-1.7)
P 		236.2
(-1.7)
P 		170.4
(-3)
P 		193.6
(-2.6)
P 		315
C 463
(3)
P 		330.1
(0.5)
P 		250.9
(-1.1)
P 		255.3
(-1)
P 		215.3
(-1.8)
P 		150.2
(-3)
P 		313
avr 433.1
(3) 		334.6
(0.7) 		257.7
(-1.1) 		226.1
(-1.9) 		204.5
(-2.4) 		179.5
(-3) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 151.7
(3)
P 		77.5
(-1)
P 		69.9
(-1.4)
P 		40.5
(-3)
P 		111
B 114.9
(3)
P 		83.5
(0.3)
P 		64.7
(-1.3)
P 		45.1
(-3)
P 		70
C 121
(3)
P 		102.9
(1.7)
P 		99.3
(1.4)
P 		37.6
(-3)
P 		83
avr 129.2
(3) 		88
(0.2) 		78
(-0.5) 		41.1
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 64.7
(-1.7)
P 		127.6
(3)
P 		61.8
(-1.9)
P 		47.4
(-3)
P 		68.8
(-1.4)
P 		47.4
(-3)
P 		80
B 168.7
(3)
P 		104.8
(-0.5)
P 		57.9
(-3)
P 		62.6
(-2.7)
P 		69.2
(-2.4)
P 		72.2
(-2.2)
P 		111
C 171.6
(3)
P 		100.7
(-0.5)
P 		63.9
(-2.3)
P 		51.3
(-3)
P 		69.7
(-2)
P 		50.3
(-3)
P 		121
avr 135
(3) 		111
(1.2) 		61.2
(-2.5) 		53.8
(-3) 		69.2
(-1.9) 		56.6
(-2.8) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186640_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10997
Sequence Type Exemplar sequence
Representative Public ID Y38C1AB.4 NCBI
Target Description Y38C1AB.4 /REP_DB=WormBase Gene ID /WP=CE25254 /TR=Q9N531 /GB=AAF60499.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067449(11), NM_067455(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067449 NCBI Caenorhabditis elegans Y38C1AB.4 (Y38C1AB.4) mRNA, complete cds. 11/11 A 
NM_067455 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-5) (frm-5) mRNA, complete cds. 11/11 A 
SNAP00000032135 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11313:14926:1 11/11 None
SNAP00000032143 ENSEMBL cdna:SNAP chromosome:CEL140:IV:32953:36566:-1 11/11 None
GENEFINDER00000032146 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:32953:36611:-1 11/11 None
GENEFINDER00000032154 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11268:14926:1 11/11 None
Y38C1AB.4 ENSEMBL cdna:known chromosome:CEL140:IV:695:14926:1 gene:Y38C1AB.4 11/11 A 
Y38C1AB.8 ENSEMBL cdna:known chromosome:CEL140:IV:32953:47184:-1 gene:Y38C1AB.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:694-14926(+) 99.05 99.15
chrIV:32952-47184(-) 99.03 99.12
Public Domain and Genome References
Gene Title

Gene Symbol frm-5
Ensembl Y38C1AB.4
Y38C1AB.8
Entrez Gene 176821 Entrez gene
176822 Entrez gene
SwissProt Q9N531 EMBL-EBI
Q9N530 EMBL-EBI
WormBase CE25254 Wormbase
CE27782 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5856 cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60499 Hypothetical protein Y38C1AB.4 [Caenorhabditis elegans] ref|NP_499850.1| Y38C1AB.4 [Caenorhabditis elegans] 0.0
blast CAE58494 Hypothetical protein CBG01639 [Caenorhabditis briggsae] 0.0
blast AAF60494 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 5 [Caenorhabditis elegans] ref|NP_499856.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-5) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 2.6E-5
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Sequence
Target Sequence 
>C. ELEGANS:186640_S_AT
atccctgagtggccgagtatgcatcaaacagcttacatcggagccagcaatctctcatcc
tgatatccgtgtgggtgatgtgttgctctatgtgaatggaatcgcagtcgaaggaaaagt
acatcaggaggtggtggcgatgctccgtggtggtggagatcgagtggttcttggagttca
aaggccccctccggcatactcggatcagcatcatgcgagttcgacatctgcttcggctcc
gctgatatcggttatgcttctgaagaagccgatggcaacactaggcctctcccttgccaa
acgtacaatgagtgacggaatcttcattcgaaacattgctcaagactcggcggcctcctc
tgaaggtactctccgagttggtgatcgtcttgtgtcccttgacggagaacctgtggacgg
attcacaccagcgactattttggaaaagctgaagctggtacagggtcccgtacag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCCTGAGTGGCCGAGTATGCATC 531 37 3615 Antisense
TTACATCGGAGCCAGCAATCTCTCA 495 681 3647 Antisense
CTCTCATCCTGATATCCGTGTGGGT 324 229 3666 Antisense
TCATGCGAGTTCGACATCTGCTTCG 123 623 3825 Antisense
TCGGCTCCGCTGATATCGGTTATGC 479 605 3847 Antisense
GAAGCCGATGGCAACACTAGGCCTC 548 341 3879 Antisense
CTCTCCCTTGCCAAACGTACAATGA 238 231 3901 Antisense
GTACTCTCCGAGTTGGTGATCGTCT 585 459 3980 Antisense
GTCTTGTGTCCCTTGACGGAGAACC 437 465 4001 Antisense
ACCTGTGGACGGATTCACACCAGCG 229 89 4023 Antisense
GAAGCTGGTACAGGGTCCCGTACAG 557 317 4065 Antisense
Sequence Source Affymetrix Proprietary Database