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[186600_at]

Description ZK945.4 /REP_DB=WormBase Gene ID /WP=CE01735 /TR=SW:Q09381 /GB=CAA88438.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 85      4grp_total > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 70.4
(-2.2)
A 		54.1
(-3)
A 		73.4
(-2)
A 		171.9
(3)
P 		118
B 86.3
(0.8)
A 		38.3
(-2)
A 		22.2
(-3)
A 		123.1
(3)
P 		101
C 66
(-2.8)
A 		61.5
(-3)
A 		62.3
(-3)
A 		173.8
(3)
P 		112
avr 74.2
(-1.7) 		51.3
(-3) 		52.6
(-2.9) 		156.3
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 97.4
(-2.2)
A 		90.8
(-3)
A 		132.8
(2)
P 		140.2
(2.8)
P 		131.2
(1.8)
P 		141.6
(3)
P 		51
B 76.9
(-3)
A 		102.3
(-0.2)
P 		86.4
(-1.9)
A 		79.5
(-2.7)
P 		122
(2)
P 		130.8
(3)
P 		54
C 46.6
(-3)
A 		109.8
(0.6)
P 		133.1
(1.9)
A 		120.3
(1.2)
P 		118.6
(1.1)
P 		151.6
(3)
P 		105
avr 73.6
(-3) 		101
(-0.6) 		117.4
(0.9) 		113.3
(0.5) 		123.9
(1.5) 		141.3
(3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 25.6
(3)
A 		11.7
(-3)
A 		19.2
(0.2)
A 		25.2
(2.8)
P 		14
B 27.5
(3)
A 		9.5
(-1.8)
A 		5.1
(-3)
A 		26.2
(2.7)
P 		22
C 21.9
(-0.8)
A 		17.7
(-3)
A 		21.7
(-0.9)
A 		29.3
(3)
P 		12
avr 25
(2.2) 		13
(-3) 		15.3
(-2) 		26.9
(3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 18
(-3)
A 		32.3
(1)
A 		28.9
(0)
P 		35.6
(1.9)
P 		39.6
(3)
P 		34.5
(1.6)
P 		22
B 26.7
(-1.8)
A 		34.1
(-0.3)
P 		21
(-3)
A 		21.1
(-3)
P 		49.6
(3)
P 		48.8
(2.8)
P 		29
C 17.3
(-3)
A 		33.5
(-0.1)
P 		33.9
(-0)
A 		24.2
(-1.8)
P 		38.4
(0.8)
P 		50.8
(3)
P 		34
avr 20.7
(-3) 		33.3
(0.1) 		27.9
(-1.2) 		27
(-1.4) 		42.5
(2.5) 		44.7
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186600_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3824
Sequence Type Exemplar sequence
Representative Public ID ZK945.4 NCBI
Target Description ZK945.4 /REP_DB=WormBase Gene ID /WP=CE01735 /TR=SW:Q09381 /GB=CAA88438.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063778(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063778 NCBI Caenorhabditis elegans ZK945.4 (ZK945.4) mRNA, complete cds. 10/11 None
SNAP00000015335 ENSEMBL cdna:SNAP chromosome:CEL140:II:10103024:10105601:-1 10/11 None
GENEFINDER00000015347 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10103024:10105607:-1 10/11 None
ZK945.4 ENSEMBL cdna:known chromosome:CEL140:II:10103024:10103930:-1 gene:ZK945.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10103020-10103927(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK945.4
Entrez Gene 174573 Entrez gene
WormBase CE35922 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88438 Hypothetical protein ZK945.4 [Caenorhabditis elegans] ref|NP_496179.2| ZK945.4 [Caenorhabditis elegans] 1.0E-130
blast Q09381 Hypothetical protein ZK945.4 in chromosome II 1.0E-127
blast CAA88443 Hypothetical protein ZK945.5 [Caenorhabditis elegans] ref|NP_496180.1| ZK945.5 [Caenorhabditis elegans] sp|Q09623|YS85_CAEEL Hypothetical RING finger protein ZK945.5 in chromosome II 1.0E-119
blast CAA88443 Hypothetical protein ZK945.5 [Caenorhabditis elegans] ref|NP_496180.1| ZK945.5 [Caenorhabditis elegans] sp|Q09623|YS85_CAEEL Hypothetical RING finger protein ZK945.5 in chromosome II 1.0E-118
Sequence
Target Sequence 
>C. ELEGANS:186600_AT
ttatagagttcctggctgactgctcgacgtgtattgttcatgttctatcacaacgtccat
tgcaaattgagatgataccggcatttagattggaaattcgagagtgtcaccgaaacgaag
tgagaaagttcatcgaaaaccacagcactcgaatttcgtcccgatcaaacattcttcgtt
ataattgcgactttttagaaatcattgacagaactgaagatcttacgatttattctgaag
gattgcgcttaataatgatagttgatctgctcgatggtgatcgtgatcgatactttgtcg
ctctggagcagttggaagaagctgtagcatatgaaaaaataattgttggtctgaaaagct
attctcgtggacatggcgaagctgcatcgaattttctcactaagctggcggatctccata
acaaaatgccaaaaatcaccgttgtcgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATAGAGTTCCTGGCTGACTGCTC 524 673 278 Antisense
GCTGACTGCTCGACGTGTATTGTTC 236 297 292 Antisense
GTTCATGTTCTATCACAACGTCCAT 222 443 313 Antisense
AAAACCACAGCACTCGAATTTCGTC 33 133 413 Antisense
TTCGTCCCGATCAAACATTCTTCGT 525 695 432 Antisense
GATAGTTGATCTGCTCGATGGTGAT 407 425 534 Antisense
GATCGATACTTTGTCGCTCTGGAGC 56 417 562 Antisense
GAAAAGCTATTCTCGTGGACATGGC 75 355 630 Antisense
GAAGCTGCATCGAATTTTCTCACTA 287 343 655 Antisense
TTTCTCACTAAGCTGGCGGATCTCC 525 671 670 Antisense
AATGCCAAAAATCACCGTTGTCGTA 106 165 702 Antisense
Sequence Source Affymetrix Proprietary Database