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[186582_s_at]

Description Y47G6A.24 /REP_DB=WormBase Gene ID /WP=CE24370 /TR=Q9N3R7 /GB=AAF60669.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 48      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 86.2
(-2.4)
A 		107.7
(-1.2)
A 		179
(3)
A 		76.3
(-3)
A 		103
B 45.1
(-3)
A 		122
(3)
A 		98.1
(1.1)
A 		105
(1.7)
A 		77
C 53.7
(-2.9)
A 		160.2
(1.5)
A 		195.9
(3)
M 		51.9
(-3)
A 		144
avr 61.7
(-3) 		130
(1.3) 		157.7
(3) 		77.7
(-2) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 235
1 2 3 4 5 6 range
A 136.6
(0.1)
A 		92.3
(-2.2)
A 		75.8
(-3)
A 		102.1
(-1.7)
A 		193.8
(3)
A 		162.4
(1.4)
P 		118
B 114.3
(-1.3)
P 		48.9
(-3)
A 		93.7
(-1.9)
A 		50.7
(-3)
A 		283.7
(3)
P 		231.9
(1.7)
P 		235
C 122.6
(-1.1)
M 		65.2
(-3)
A 		68
(-2.9)
A 		120
(-1.2)
A 		187
(1)
A 		249
(3)
P 		184
avr 124.5
(-0.8) 		68.8
(-3) 		79.2
(-2.6) 		90.9
(-2.1) 		221.5
(3) 		214.4
(2.7) 		153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 31.4
(0.4)
A 		23.4
(-0.9)
A 		46.8
(3)
A 		11.2
(-3)
A 		36
B 14.4
(-3)
A 		30.1
(3)
A 		22.7
(0.2)
A 		22.4
(0.1)
A 		16
C 17.8
(-2.1)
A 		46.1
(0.8)
A 		68.3
(3)
M 		8.8
(-3)
A 		60
avr 21.2
(-1.7) 		33.2
(0.6) 		45.9
(3) 		14.1
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 25.2
(-1.8)
A 		32.8
(-0.7)
A 		16.5
(-3)
A 		25.9
(-1.7)
A 		58.5
(3)
A 		39.5
(0.3)
P 		42
B 39.8
(-1.4)
P 		16.3
(-2.8)
A 		22.8
(-2.4)
A 		13.4
(-3)
A 		115.3
(3)
P 		86.5
(1.3)
P 		102
C 45.4
(-0.4)
M 		19.9
(-2.8)
A 		17.3
(-3)
A 		24.1
(-2.4)
A 		60.5
(0.9)
A 		83.4
(3)
P 		66
avr 36.8
(-1.2) 		23
(-2.6) 		18.9
(-3) 		21.1
(-2.8) 		78.1
(3) 		69.8
(2.2) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186582_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.131
Sequence Type Exemplar sequence
Representative Public ID Y47G6A.24 NCBI
Target Description Y47G6A.24 /REP_DB=WormBase Gene ID /WP=CE24370 /TR=Q9N3R7 /GB=AAF60669.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058783(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058783 NCBI Caenorhabditis elegans human/fission yeast MIS (minichromosome stability) homolog family member (mis-12) (mis-12) mRNA, complete cds. 11/11 None
SNAP00000033996 ENSEMBL cdna:SNAP chromosome:CEL140:I:3604681:3605696:-1 9/11 None
GENEFINDER00000034083 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3604681:3605696:-1 11/11 None
Y47G6A.24 ENSEMBL cdna:known chromosome:CEL140:I:3604586:3605696:-1 gene:Y47G6A.24 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3604680-3605696(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mis-12
Ensembl Y47G6A.24
Entrez Gene 171927 Entrez gene
SwissProt Q9N3R7 EMBL-EBI
WormBase CE24370 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491184 human/fission yeast MIS (minichromosome stability) homolog family member (mis-12) [Caenorhabditis elegans] gb|AAF60669.1| Human/fission yeast mis (minichromosome stability) homolog protein 12 [Caenorhabditis elegans] 6.0E-84
blast NP_491184 human/fission yeast MIS (minichromosome stability) homolog family member (mis-12) [Caenorhabditis elegans] gb|AAF60669.1| Human/fission yeast mis (minichromosome stability) homolog protein 12 [Caenorhabditis elegans] 1.0E-68
blast CAE66627 Hypothetical protein CBG11963 [Caenorhabditis briggsae] 3.0E-49
blast CAE66627 Hypothetical protein CBG11963 [Caenorhabditis briggsae] 9.0E-38
Sequence
Target Sequence 
>C. ELEGANS:186582_S_AT
gagcgaattgtcgggaatctctaatgaaaatcaaatctcgtttttcggattttcggccgg
ttcgttcagcgattccattttcaatatttacgtcgacgcatgggaagaagtttgcagcaa
tgagtttaagaactcattgaaaaagctcaccgacaaagaccgtctctgcttgctatcctt
cccattcagcgacaaaactaacgagaaagcattcaaaatgatgaagagattctgcgtcac
aaatatcttcagaattcccgcctctgtgactcttccagaatgcaagaaaatgctcatcgc
gtcggccaaggagcacaaaagtctgaaacaggtcgagaaggagttcgacgagaaattgga
gaaaattcgacagctccgcatcaaactggcaaaacgacatgacgagctagaagcagtgaa
caatgctattgaagtgctccatgtgatggagaagagccaagagcagcttataagccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGAATTGTCGGGAATCTCTAAT 524 385 18 Antisense
TTTTCAATATTTACGTCGACGCATG 125 677 95 Antisense
GAACTCATTGAAAAAGCTCACCGAC 428 349 147 Antisense
TCCTTCCCATTCAGCGACAAAACTA 658 583 193 Antisense
GAAGAGATTCTGCGTCACAAATATC 261 339 240 Antisense
CAAATATCTTCAGAATTCCCGCCTC 466 193 257 Antisense
TGCTCATCGCGTCGGCCAAGGAGCA 138 579 308 Antisense
GCTCCGCATCAAACTGGCAAAACGA 433 297 390 Antisense
AACGACATGACGAGCTAGAAGCAGT 36 145 410 Antisense
GCTATTGAAGTGCTCCATGTGATGG 33 305 442 Antisense
GAGCCAAGAGCAGCTTATAAGCCAG 280 385 471 Antisense
Sequence Source Affymetrix Proprietary Database