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[186576_at]

Description F45D11.10 /REP_DB=WormBase Gene ID /WP=CE23733 /TR=Q9N5U0 /GB=AAF39824.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 58      4grp_total > 47      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 25.2
(3)
A 		3.4
(-3)
A 		3.9
(-2.9)
A 		6.2
(-2.2)
A 		22
B 38.3
(3)
A 		3.3
(-2.8)
A 		1.8
(-3)
A 		3.3
(-2.8)
A 		37
C 33.8
(3)
A 		2.1
(-3)
A 		2.6
(-2.9)
A 		7.8
(-1.9)
A 		32
avr 32.4
(3) 		2.9
(-3) 		2.8
(-3) 		5.8
(-2.4) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 8
(-2.4)
A 		10.1
(-2.1)
A 		23.8
(-0.3)
A 		3.8
(-3)
A 		23.5
(-0.3)
A 		48.2
(3)
A 		44
B 24.3
(1.4)
A 		13
(-1.1)
A 		6.8
(-2.5)
A 		4.5
(-3)
A 		4.9
(-2.9)
A 		31.7
(3)
A 		27
C 40.3
(1.4)
A 		6.2
(-2.5)
A 		5.3
(-2.6)
A 		5
(-2.6)
A 		1.8
(-3)
A 		54.2
(3)
A 		52
avr 24.2
(-0.1) 		9.8
(-2.2) 		12
(-1.9) 		4.4
(-3) 		10.1
(-2.2) 		44.7
(3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 9.2
(3)
A 		0.7
(-3)
A 		1
(-2.8)
A 		0.9
(-2.9)
A 		9
B 12.2
(3)
A 		0.8
(-2.8)
A 		0.4
(-3)
A 		0.7
(-2.8)
A 		12
C 11.2
(3)
A 		0.6
(-3)
A 		0.9
(-2.8)
A 		1.3
(-2.6)
A 		11
avr 10.9
(3) 		0.7
(-3) 		0.8
(-2.9) 		1
(-2.8) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 1.5
(-2.7)
A 		3.6
(-1.5)
A 		5.2
(-0.6)
A 		1
(-3)
A 		7.1
(0.4)
A 		11.7
(3)
A 		11
B 8.5
(1.1)
A 		4.3
(-1.2)
A 		1.6
(-2.8)
A 		1.2
(-3)
A 		2
(-2.5)
A 		11.8
(3)
A 		11
C 15
(1.9)
A 		1.9
(-2.6)
A 		1.4
(-2.7)
A 		1
(-2.9)
A 		0.6
(-3)
A 		18.2
(3)
A 		18
avr 8.3
(0.4) 		3.3
(-2) 		2.7
(-2.3) 		1.1
(-3) 		3.2
(-2) 		13.9
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186576_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5239
Sequence Type Exemplar sequence
Representative Public ID F45D11.10 NCBI
Target Description F45D11.10 /REP_DB=WormBase Gene ID /WP=CE23733 /TR=Q9N5U0 /GB=AAF39824.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061475(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061475 NCBI Caenorhabditis elegans F45D11.10 (F45D11.10) mRNA, complete cds. 11/11 None
SNAP00000019550 ENSEMBL cdna:SNAP chromosome:CEL140:II:988496:990983:1 11/11 None
GENEFINDER00000019573 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:988501:991610:1 9/11 None
F45D11.10 ENSEMBL cdna:known chromosome:CEL140:II:988719:991080:1 gene:F45D11.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:988717-990982(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45D11.10
Entrez Gene 173478 Entrez gene
SwissProt Q9N5U0 EMBL-EBI
WormBase CE23733 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39824 Hypothetical protein F45D11.10 [Caenorhabditis elegans] ref|NP_493876.1| F45D11.10 [Caenorhabditis elegans] 0.0
blast AAF39824 Hypothetical protein F45D11.10 [Caenorhabditis elegans] ref|NP_493876.1| F45D11.10 [Caenorhabditis elegans] 1.0E-177
blast AAF39824 Hypothetical protein F45D11.10 [Caenorhabditis elegans] ref|NP_493876.1| F45D11.10 [Caenorhabditis elegans] 1.0E-173
blast NP_493875 C32B5.1a [Caenorhabditis elegans] gb|AAK71358.1| Hypothetical protein C32B5.1a [Caenorhabditis elegans] 9.0E-56
blast NP_493875 C32B5.1a [Caenorhabditis elegans] gb|AAK71358.1| Hypothetical protein C32B5.1a [Caenorhabditis elegans] 3.0E-44
blast NP_493875 C32B5.1a [Caenorhabditis elegans] gb|AAK71358.1| Hypothetical protein C32B5.1a [Caenorhabditis elegans] 1.0E-39
blast NP_872007 C32B5.1b [Caenorhabditis elegans] gb|AAN63439.1| Hypothetical protein C32B5.1b [Caenorhabditis elegans] 9.0E-38
Sequence
Target Sequence 
>C. ELEGANS:186576_AT
gaaaccgcaatttaccagtcaaagtcaacaggatggagttcaaaaattatttttcaagac
aagtgtgggacctgagaaatgttaaactcagaataaagaagctggaagtccataatgaaa
ttaagcaggttctaagcacacttgctccgtgcctcgatgaatctagctacccgttggaat
ccctcgaactagtgcaacagttcttcacttcggatgatttgttcaaccatcatatcatcc
aaacagcaaaggatctgagaatcatcggtatccacggaaatttccacagacttccaaatc
ttagagtgcacatccagaaggtgaacgtttccccggcaacgctaatcaaagcgatcgatt
attggttgacccgtggcaaagagattggaactcatttttcaatttcttcctgtgaaatgt
tggaggaagaagcacgagttttgtggaaggccataaggacaaactatattgatctagttg
aggaaaatcagagattaatcgatttttctcccgagccactgaaaatcaaatcacgctttt
tttcggaactctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACCGCAATTTACCAGTCAAAGT 274 357 503 Antisense
AGCAGGTTCTAAGCACACTTGCTCC 106 79 626 Antisense
CGTGCCTCGATGAATCTAGCTACCC 652 245 650 Antisense
TGGAATCCCTCGAACTAGTGCAACA 587 545 677 Antisense
GCAACAGTTCTTCACTTCGGATGAT 369 319 696 Antisense
TCAACCATCATATCATCCAAACAGC 328 631 725 Antisense
GGAAATTTCCACAGACTTCCAAATC 393 527 778 Antisense
CATCCAGAAGGTGAACGTTTCCCCG 38 3 813 Antisense
GCGATCGATTATTGGTTGACCCGTG 419 291 853 Antisense
GATTAATCGATTTTTCTCCCGAGCC 78 429 995 Antisense
AATCACGCTTTTTTTCGGAACTCTG 79 167 1031 Antisense
Sequence Source Affymetrix Proprietary Database