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[186533_s_at]

Description Y37E11B.5 /REP_DB=WormBase Gene ID /WP=CE19615 /TR=Q9TYN2 /GB=AAD12825.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 68      6grp_total > 69      4grp_total > 03      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 569
1 2 3 4 range
A 238.4
(-2.4)
P 		238.8
(-2.4)
P 		195.9
(-3)
M 		600.3
(3)
P 		404
B 237.7
(-2)
P 		209.5
(-2.3)
A 		146.5
(-3)
A 		678.3
(3)
P 		532
C 239.9
(-1.5)
P 		169.3
(-2.3)
P 		108.9
(-3)
M 		628.3
(3)
P 		519
avr 238.7
(-1.9) 		205.9
(-2.3) 		150.4
(-3) 		635.6
(3) 		485

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 239.3
(0.3)
P 		272.5
(1.7)
P 		156.2
(-3)
P 		192.8
(-1.5)
P 		186.7
(-1.8)
P 		305.5
(3)
P 		149
B 170.3
(0.5)
P 		190.2
(1.4)
P 		91.8
(-3)
P 		114.1
(-2)
P 		175.1
(0.7)
P 		226.2
(3)
P 		134
C 169.5
(0.2)
P 		172.1
(0.3)
P 		245.9
(2.5)
P 		66.6
(-3)
A 		176.5
(0.4)
P 		261
(3)
P 		194
avr 193
(-0.1) 		211.6
(0.7) 		164.6
(-1.3) 		124.5
(-3) 		179.4
(-0.6) 		264.2
(3) 		140
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 86.8
(2.8)
P 		51.8
(-2.9)
P 		51.2
(-3)
M 		87.9
(3)
P 		37
B 75.8
(-0.7)
P 		51.7
(-2)
A 		33.9
(-3)
A 		144.6
(3)
P 		111
C 79.4
(0.7)
P 		48.7
(-2.1)
P 		38
(-3)
M 		105.9
(3)
P 		68
avr 80.7
(0.3) 		50.7
(-2.2) 		41
(-3) 		112.8
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 44.1
(-2)
P 		96.9
(3)
P 		34
(-3)
P 		48.9
(-1.6)
P 		56.3
(-0.9)
P 		74.4
(0.9)
P 		63
B 59.3
(0.6)
P 		63.3
(1)
P 		22.3
(-3)
P 		30.2
(-2.2)
P 		71.1
(1.7)
P 		84.3
(3)
P 		62
C 62.8
(1)
P 		52.5
(0.2)
P 		62.6
(1)
P 		13.4
(-3)
A 		57.1
(0.5)
P 		87.4
(3)
P 		74
avr 55.4
(-0.1) 		70.9
(1.7) 		39.6
(-2) 		30.8
(-3) 		61.5
(0.6) 		82
(3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186533_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11161
Sequence Type Exemplar sequence
Representative Public ID Y37E11B.5 NCBI
Target Description Y37E11B.5 /REP_DB=WormBase Gene ID /WP=CE19615 /TR=Q9TYN2 /GB=AAD12825.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067978(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067978 NCBI Caenorhabditis elegans Y37E11B.5 (Y37E11B.5) mRNA, complete cds. 11/11 None
SNAP00000044694 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3594256:3599234:-1 11/11 None
GENEFINDER00000044703 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3584730:3598541:-1 11/11 None
Y37E11B.5 ENSEMBL cdna:known chromosome:CEL140:IV:3593965:3598547:-1 gene:Y37E11B.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3594261-3598547(-) 99.94 100.0
Public Domain and Genome References
Gene Title
Ensembl Y37E11B.5
Entrez Gene 177120 Entrez gene
SwissProt Q9TYN2 EMBL-EBI
WormBase CE19615 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:252968_AT dihydrouridine synthase family protein at
CANINE_2:CFAAFFX.28653.1.S1_S_AT similar to dihydrouridine synthase 3-like cfa
DROSOPHILA_2:1640143_AT dm
DROSGENOME1:146511_AT dm
CHICKEN:GGA.15947.1.S1_AT dihydrouridine synthase 3-like (S. cerevisiae) gga
CHICKEN:GGA.15947.1.S1_S_AT dihydrouridine synthase 3-like (S. cerevisiae) gga
CHICKEN:GGAAFFX.25199.1.S1_AT dihydrouridine synthase 3-like (S. cerevisiae) gga
HU35KSUBA:RC_AA454923_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
HU35KSUBC:RC_AA101419_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
U133_X3P:HS.284297.2.S1_3P_A_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
HG-U133_PLUS_2:224966_S_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
HG-U133B:224966_S_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
HG-U95C:65820_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
HG-U95E:78759_AT dihydrouridine synthase 3-like (S. cerevisiae) hs
HG-U95D:90045_S_AT Dihydrouridine synthase 3-like (S. cerevisiae) hs
HG-U95E:91155_AT Dihydrouridine synthase 3-like (S. cerevisiae) hs
U133_X3P:HS.284297.1.A1_3P_AT Dihydrouridine synthase 3-like (S. cerevisiae) hs
MG-U74BV2:162912_AT dihydrouridine synthase 3-like (S. cerevisiae) mm
MOE430A:1424066_AT dihydrouridine synthase 3-like (S. cerevisiae) mm
MOUSE430A_2:1424066_AT dihydrouridine synthase 3-like (S. cerevisiae) mm
MOUSE430_2:1424066_AT dihydrouridine synthase 3-like (S. cerevisiae) mm
MOE430B:1459805_X_AT dihydrouridine synthase 3-like (S. cerevisiae) mm
MOUSE430_2:1459805_X_AT dihydrouridine synthase 3-like (S. cerevisiae) mm
MU19KSUBA:TC21262_AT Dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l), mRNA mm
MU19KSUBA:TC21262_G_AT Dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l), mRNA mm
RG-U34C:RC_AI102764_AT Similar to putative zinc finger protein rn
RG-U34C:RC_AI177187_AT Similar to putative zinc finger protein rn
RAE230A:1374979_AT Similar to putative zinc finger protein rn
RAT230_2:1374979_AT Similar to putative zinc finger protein rn
RAE230B:1391053_AT Similar to putative zinc finger protein rn
RAT230_2:1391053_AT Similar to putative zinc finger protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8033 tRNA processing inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
50660 FAD binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD12825 Hypothetical protein Y37E11B.5 [Caenorhabditis elegans] ref|NP_500379.1| Y37E11B.5 [Caenorhabditis elegans] 0.0
blast CAE63785 Hypothetical protein CBG08326 [Caenorhabditis briggsae] 0.0
blast AAD12824 Taf (tbp-associated transcription factor) family protein 2 [Caenorhabditis elegans] ref|NP_500378.1| TAF (TBP-associated transcription factor) family member (taf-2) [Caenorhabditis elegans] 0.0
blast NP_064560 dihydrouridine synthase 3-like [Homo sapiens] gb|AAH09973.1| Dihydrouridine synthase 3-like [Homo sapiens] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR001269 EMBL-EBI Protein of unknown function UPF0034 1.5E-54
Pfam IPR001269 EMBL-EBI Protein of unknown function UPF0034 1.5E-54
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 5.0E-6
Sequence
Target Sequence 
>C. ELEGANS:186533_S_AT
caaagcgcccgaatacgtggagcacatgaaaaagcactcatggcatacacctgacctcat
cacattccacccacggagcaaagagcaacgatacacacggctcgccaattgggatttcac
atttccagttcaagaagctacaaaacctgttcccttatgggtttgtggagatattctgag
ctgggaagactattacgagcggctggagcaatatccagttaatggaattatgattggacg
tggagcactcattaaaccatggattttcactgaaattgatgagagacggacttgggatat
tagttcaactgagagatttgatttactcaaaaaattcgtcgactacggtctcgaccactg
gggatccgatgatgcaggcgtcgaacgtacccgtcgattccttctcgagtttctatcatt
ccagtgccgctacatccctgtcggaatccttgaacgacttccccaacggatcaacgatcg
cccaccaagattctgcggtcgaaatgagcttgaaacgttgctctcctcacaaaaagccga
cgattggatcgaaatttcgactcggctgctgggaccgacgccggaaggatttcagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGCGCCCGAATACGTGGAGCAC 104 193 1050 Antisense
AAAGCACTCATGGCATACACCTGAC 597 123 1080 Antisense
CACATTCCACCCACGGAGCAAAGAG 87 195 1110 Antisense
CACGGCTCGCCAATTGGGATTTCAC 141 199 1145 Antisense
GAAGCTACAAAACCTGTTCCCTTAT 249 337 1183 Antisense
TCTCGACCACTGGGGATCCGATGAT 473 609 1398 Antisense
GATGATGCAGGCGTCGAACGTACCC 417 411 1417 Antisense
ATTCCTTCTCGAGTTTCTATCATTC 132 9 1446 Antisense
TCGGAATCCTTGAACGACTTCCCCA 703 605 1490 Antisense
GTTGCTCTCCTCACAAAAAGCCGAC 539 439 1566 Antisense
GACCGACGCCGGAAGGATTTCAGTT 429 375 1622 Antisense
Sequence Source Affymetrix Proprietary Database