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[186529_at]

Description Y116A8C.28 /REP_DB=WormBase Gene ID /WP=CE24150 /TR=Q9U2S1 /GB=CAB55156.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 64      6grp_total > 63      4grp_total > 54      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 927
1 2 3 4 range
A 1244.3
(3)
P 		337.2
(-3)
P 		555.7
(-1.6)
P 		361.9
(-2.8)
P 		907
B 686
(3)
P 		614
(1.8)
P 		589.6
(1.4)
P 		334.6
(-3)
P 		351
C 928.7
(3)
P 		699
(0.7)
P 		585.9
(-0.4)
P 		317.7
(-3)
P 		611
avr 953
(3) 		550.1
(-0.9) 		577.1
(-0.7) 		338.1
(-3) 		615

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 501
1 2 3 4 5 6 range
A 645.1
(-3)
P 		732.9
(-1.9)
P 		1146.3
(3)
P 		991.8
(1.2)
P 		878
(-0.2)
P 		845.2
(-0.6)
P 		501
B 736
(-2.9)
P 		732.3
(-3)
P 		913.9
(3)
P 		879.5
(1.9)
P 		781.5
(-1.4)
P 		790.4
(-1.1)
P 		182
C 726.1
(-3)
P 		745.9
(-2.5)
P 		908.4
(1.2)
P 		989.2
(3)
P 		766.4
(-2.1)
P 		742.3
(-2.6)
P 		263
avr 702.4
(-3) 		737
(-2.3) 		989.5
(3) 		953.5
(2.2) 		808.6
(-0.8) 		792.6
(-1.1) 		287
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 400
1 2 3 4 range
A 453
(3)
P 		73.2
(-2.7)
P 		145.3
(-1.6)
P 		53
(-3)
P 		400
B 218.8
(3)
P 		151.7
(0.3)
P 		136.6
(-0.3)
P 		71.3
(-3)
P 		148
C 307.4
(3)
P 		201
(0.5)
P 		204.2
(0.6)
P 		53.6
(-3)
P 		254
avr 326.4
(3) 		142
(-1.1) 		162
(-0.7) 		59.3
(-3) 		267

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 199
1 2 3 4 5 6 range
A 118.9
(-3)
P 		260.7
(2.8)
P 		249.6
(2.4)
P 		251.5
(2.4)
P 		265
(3)
P 		205.8
(0.6)
P 		146
B 256.1
(-0.9)
P 		243.7
(-1.6)
P 		221.9
(-3)
P 		233
(-2.3)
P 		317.5
(3)
P 		294.7
(1.6)
P 		96
C 269.1
(3)
P 		227.6
(-0.5)
P 		231.2
(-0.2)
P 		198.9
(-3)
P 		248
(1.2)
P 		248.5
(1.2)
P 		70
avr 214.7
(-3) 		244
(-0.2) 		234.2
(-1.1) 		227.8
(-1.7) 		276.8
(3) 		249.7
(0.4) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186529_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8891
Sequence Type Exemplar sequence
Representative Public ID Y116A8C.28 NCBI
Target Description Y116A8C.28 /REP_DB=WormBase Gene ID /WP=CE24150 /TR=Q9U2S1 /GB=CAB55156.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070630, NM_182292
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000004177 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:17096438:17100198:-1 None
NM_182292 NCBI Caenorhabditis elegans Y116A8C.28b (Y116A8C.28) mRNA, complete cds. None
Y116A8C.28a.2 ENSEMBL cdna:known chromosome:CEL140:IV:17096345:17100203:-1 gene:Y116A8C.28 None
Y116A8C.28a.1 ENSEMBL cdna:known chromosome:CEL140:IV:17096345:17099413:-1 gene:Y116A8C.28 None
Y116A8C.28b ENSEMBL cdna:known chromosome:CEL140:IV:17096353:17100203:-1 gene:Y116A8C.28 None
SNAP00000004101 ENSEMBL cdna:SNAP chromosome:CEL140:IV:17096483:17100198:-1 None
NM_070630 NCBI Caenorhabditis elegans Y116A8C.28a (Y116A8C.28) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070628 1/11 Cross Hyb Matching Probes None
SNAP00000004100 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004169 1/11 Cross Hyb Matching Probes None
Y116A8C.27 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17095985-17100204(-) 93.23 100.0
Public Domain and Genome References
Ensembl Y116A8C.28
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T31522 hypothetical protein Y116A8C.28 - Caenorhabditis elegans 0.0
blast T31522 hypothetical protein Y116A8C.28 - Caenorhabditis elegans 1.0E-149
blast CAE57459 Hypothetical protein CBG00424 [Caenorhabditis briggsae] 1.0E-103
blast CAE57459 Hypothetical protein CBG00424 [Caenorhabditis briggsae] 2.0E-91
blast AAK39291 Beta carbonic anhydrase protein 1, isoform a [Caenorhabditis elegans] ref|NP_741809.1| Beta Carbonic Anhydrase family member (bca-1) [Caenorhabditis elegans] 4.0E-83
blast CAD56251 Hypothetical protein Y116A8C.28a [Caenorhabditis elegans] ref|NP_503031.2| Y116A8C.28a [Caenorhabditis elegans] 3.0E-68
blast AAK39291 Beta carbonic anhydrase protein 1, isoform a [Caenorhabditis elegans] ref|NP_741809.1| Beta Carbonic Anhydrase family member (bca-1) [Caenorhabditis elegans] 2.0E-67
blast CAE57459 Hypothetical protein CBG00424 [Caenorhabditis briggsae] 3.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR001765 EMBL-EBI Carbonic anhydrase, prokaryotic and plant 3.9E-15
Pfam IPR001765 EMBL-EBI Carbonic anhydrase, prokaryotic and plant 1.4E-9
Sequence
Target Sequence 
>C. ELEGANS:186529_AT
gttctcattctcggcagtaattgacccggaagacaagctgaatgtcgaggataagctctc
acaaattaatacacttcaacaattggaaaatgtggccagtcatggatttttgaagagaaa
acctttttttttaatttctgaaataggaaattatgcccctcaaatagattttttttattc
aaaattcaaaacatattcaaaacacattttttcaggaattcctcgtgtctcaaactgtcg
atcttcatgcgatgtgatgctaaaacaatttccaagtaaaaaattatgtattcagtgggc
aagcagcggtgaaagagaaatgcacatgttcagcaaacccaacaaacaattcgttctggt
cgatgaatcgaatgtcgaagaattgattgatgagtgtcctatcgttgtaattttgaattt
taaatttttttttttagaaaaattcaatttcagtgctcaaatggcgtccaatcttggccg
tgttgctcggcgatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCTCATTCTCGGCAGTAATTGAC 650 441 789 Antisense
GAATGTCGAGGATAAGCTCTCACAA 569 331 828 Antisense
AAATGTGGCCAGTCATGGATTTTTG 233 121 876 Antisense
GGAAATTATGCCCCTCAAATAGATT 210 527 934 Antisense
TTCAGGAATTCCTCGTGTCTCAAAC 453 687 999 Antisense
TGTCTCAAACTGTCGATCTTCATGC 15 559 1014 Antisense
AATGCACATGTTCAGCAAACCCAAC 2 165 1107 Antisense
CAAACCCAACAAACAATTCGTTCTG 690 191 1122 Antisense
ATGAGTGTCCTATCGTTGTAATTTT 555 45 1178 Antisense
CAAATGGCGTCCAATCTTGGCCGTG 326 193 1246 Antisense
ATCTTGGCCGTGTTGCTCGGCGATT 86 31 1259 Antisense
Sequence Source Affymetrix Proprietary Database