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[186525_at]

Description VF13D12L.3 /REP_DB=WormBase Gene ID /WP=CE21229 /TR=Q9XTB4 /GB=CAA88951.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 33      6grp_total > 63      4grp_total > 63      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 495
1 2 3 4 range
A 1130.8
(3)
P 		767.8
(-3)
P 		866.3
(-1.4)
P 		834.1
(-1.9)
P 		363
B 912.5
(-0)
P 		802.2
(-3)
P 		1024.9
(3)
P 		816.8
(-2.6)
P 		223
C 1132.9
(3)
P 		638.4
(-3)
P 		934.2
(0.6)
P 		640.4
(-3)
P 		495
avr 1058.7
(3) 		736.1
(-3) 		941.8
(0.8) 		763.8
(-2.5) 		323

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1705
1 2 3 4 5 6 range
A 906.5
(-3)
P 		1705.4
(1.7)
P 		1924.6
(3)
P 		1850
(2.6)
P 		1258.7
(-0.9)
P 		1415.3
(-0)
P 		1018
B 868.8
(-3)
P 		1436.4
(-0.1)
P 		2058
(3)
P 		1532.4
(0.3)
P 		1577.5
(0.6)
P 		1368.1
(-0.5)
P 		1189
C 899.6
(-3)
P 		1451.6
(-1)
P 		2573.4
(3)
P 		1203.5
(-1.9)
P 		1449.6
(-1)
P 		1481.4
(-0.9)
P 		1674
avr 891.6
(-3) 		1531.1
(-0) 		2185.3
(3) 		1528.6
(-0) 		1428.6
(-0.5) 		1421.6
(-0.5) 		1294
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 304
1 2 3 4 range
A 411.7
(3)
P 		166.7
(-2.1)
P 		226.5
(-0.8)
P 		122.1
(-3)
P 		290
B 291
(3)
P 		198.1
(-1.8)
P 		237.5
(0.3)
P 		174.1
(-3)
P 		117
C 375
(3)
P 		183.6
(-1.3)
P 		325.6
(1.9)
P 		108
(-3)
P 		267
avr 359.2
(3) 		182.8
(-1.7) 		263.2
(0.4) 		134.7
(-3) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 488
1 2 3 4 5 6 range
A 167.1
(-3)
P 		606.5
(3)
P 		419.1
(0.4)
P 		469.2
(1.1)
P 		379.8
(-0.1)
P 		344.6
(-0.6)
P 		439
B 302.3
(-3)
P 		478.1
(0.1)
P 		499.8
(0.5)
P 		405.9
(-1.2)
P 		640.9
(3)
P 		510.2
(0.7)
P 		339
C 333.4
(-1.7)
P 		442.9
(-0.1)
P 		655
(3)
P 		242
(-3)
P 		469.1
(0.3)
P 		495.9
(0.7)
P 		413
avr 267.6
(-3) 		509.2
(2.6) 		524.6
(3) 		372.4
(-0.6) 		496.6
(2.3) 		450.2
(1.3) 		257
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186525_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3500
Sequence Type Exemplar sequence
Representative Public ID VF13D12L.3 NCBI
Target Description VF13D12L.3 /REP_DB=WormBase Gene ID /WP=CE21229 /TR=Q9XTB4 /GB=CAA88951.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064099(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064099 NCBI Caenorhabditis elegans VF13D12L.3 (VF13D12L.3) mRNA, complete cds. 11/11 None
SNAP00000015025 ENSEMBL cdna:SNAP chromosome:CEL140:II:11710902:11713402:1 11/11 None
GENEFINDER00000015050 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11710024:11713402:1 11/11 None
VF13D12L.3 ENSEMBL cdna:known chromosome:CEL140:II:11705919:11713506:1 gene:VF13D12L.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11705948-11713399(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl VF13D12L.3
Entrez Gene 174795 Entrez gene
SwissProt Q9XTB4 EMBL-EBI
WormBase CE21229 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1636843_A_AT dm
DROSGENOME1:152050_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88951 Hypothetical protein VF13D12L.3 [Caenorhabditis elegans] emb|CAA22133.1| Hypothetical protein VF13D12L.3 [Caenorhabditis elegans] ref|NP_496500.1| VF13D12L.3 [Caenorhabditis elegans] 0.0
blast CAE59707 Hypothetical protein CBG03139 [Caenorhabditis briggsae] 0.0
blast EAA44260 ENSANGP00000025275 [Anopheles gambiae str. PEST] ref|XP_316604.2| ENSANGP00000025275 [Anopheles gambiae str. PEST] 1.0E-110
blast T20825 hypothetical protein F13D12.1 - Caenorhabditis elegans 3.0E-93
blast CAA88951 Hypothetical protein VF13D12L.3 [Caenorhabditis elegans] emb|CAA22133.1| Hypothetical protein VF13D12L.3 [Caenorhabditis elegans] ref|NP_496500.1| VF13D12L.3 [Caenorhabditis elegans] 1.0E-82
blast T20825 hypothetical protein F13D12.1 - Caenorhabditis elegans 9.0E-82
blast CAA88951 Hypothetical protein VF13D12L.3 [Caenorhabditis elegans] emb|CAA22133.1| Hypothetical protein VF13D12L.3 [Caenorhabditis elegans] ref|NP_496500.1| VF13D12L.3 [Caenorhabditis elegans] 1.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR003767 EMBL-EBI Malate/L-lactate dehydrogenase 2.0E-24
Pfam IPR003767 EMBL-EBI Malate/L-lactate dehydrogenase 9.8E-13
Pfam IPR003767 EMBL-EBI Malate/L-lactate dehydrogenase 7.3E-83
Sequence
Target Sequence 
>C. ELEGANS:186525_AT
aggagaatccagtaccattgtcatggggagttggagaaggtggcaaagaaacaactgatc
cgacgaaggttctgtatggaggaggactacttccattgggtggagtcgaagttagcggcg
gctacaagggatatggtctgagttcgatgatcgagatattctgtggaatattggctggag
ctcattggggtcctcacgttcgaaaatggatgagcacaaagtcggaagctgatttgggac
aatgcttcgtggcaatcgacccagaagccttcgcaccaggctttgcagaccgacttcagg
actttatgcagacgatgagagcactgccaacgtcatcaccttcgttcaaagtggaagtcg
ccggggatatggaaagacgtcacgaggcgcttgtggaacaactagggggaatcccatatc
acaagaatcagatcactttcgtgaacgatctagccgccaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGAGAATCCAGTACCATTGTCATG 702 55 722 Antisense
GAGGAGGACTACTTCCATTGGGTGG 567 403 800 Antisense
GTGGAGTCGAAGTTAGCGGCGGCTA 506 489 821 Antisense
ATTGGCTGGAGCTCATTGGGGTCCT 704 1 891 Antisense
TTGGGGTCCTCACGTTCGAAAATGG 106 591 906 Antisense
GACAATGCTTCGTGGCAATCGACCC 15 365 959 Antisense
CAGACCGACTTCAGGACTTTATGCA 390 203 1007 Antisense
CCAACGTCATCACCTTCGTTCAAAG 247 275 1048 Antisense
AGACGTCACGAGGCGCTTGTGGAAC 75 71 1096 Antisense
GGGGGAATCCCATATCACAAGAATC 573 497 1126 Antisense
TTTCGTGAACGATCTAGCCGCCAAG 705 661 1158 Antisense
Sequence Source Affymetrix Proprietary Database