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[186522_s_at]

Description T28A11.20 /REP_DB=WormBase Gene ID /WP=CE14317 /TR=P91519 /GB=AAC48128.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 16      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 4.2
(-3)
A 		16.3
(-0.7)
A 		23.2
(0.7)
A 		35.1
(3)
A 		31
B 1
(-3)
A 		7.9
(-1.6)
A 		5
(-2.2)
A 		29.7
(3)
A 		29
C 1.3
(-3)
A 		1.3
(-3)
A 		12.5
(-0.6)
A 		29
(3)
A 		28
avr 2.2
(-3) 		8.5
(-1.7) 		13.6
(-0.6) 		31.3
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 33.7
(3)
A 		14.4
(-0.6)
A 		20.9
(0.6)
A 		1.6
(-2.9)
A 		1.3
(-3)
A 		4.5
(-2.4)
A 		32
B 1.5
(-3)
A 		11.9
(0.8)
A 		4.2
(-2)
A 		1.4
(-3)
A 		11.2
(0.5)
A 		18.2
(3)
A 		17
C 0.7
(-3)
A 		4.7
(-2.2)
A 		21.3
(1.1)
A 		27.9
(2.4)
A 		8.3
(-1.5)
A 		30.8
(3)
A 		30
avr 12
(-0.2) 		10.3
(-1.1) 		15.5
(1.7) 		10.3
(-1.1) 		6.9
(-3) 		17.8
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 1.5
(-3)
A 		3.5
(-0.4)
A 		6.1
(3)
A 		5.1
(1.7)
A 		5
B 0.3
(-3)
A 		1.9
(-1.4)
A 		1.2
(-2.1)
A 		6.3
(3)
A 		6
C 0.4
(-3)
A 		0.4
(-3)
A 		4.4
(2.3)
A 		4.9
(3)
A 		5
avr 0.7
(-3) 		1.9
(-1.5) 		3.9
(1.1) 		5.4
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 6.2
(3)
A 		5.1
(1.9)
A 		4.5
(1.2)
A 		0.4
(-3)
A 		0.4
(-3)
A 		1.1
(-2.3)
A 		6
B 0.5
(-2.9)
A 		4
(0.4)
A 		1
(-2.4)
A 		0.4
(-3)
A 		4.6
(0.9)
A 		6.8
(3)
A 		6
C 0.3
(-3)
A 		1.4
(-2.3)
A 		5.4
(0.1)
A 		5.6
(0.2)
A 		2.7
(-1.6)
A 		10.3
(3)
A 		10
avr 2.3
(-2.7) 		3.5
(-0.9) 		3.6
(-0.8) 		2.1
(-3) 		2.6
(-2.3) 		6.1
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186522_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15284
Sequence Type Exemplar sequence
Representative Public ID T28A11.20 NCBI
Target Description T28A11.20 /REP_DB=WormBase Gene ID /WP=CE14317 /TR=P91519 /GB=AAC48128.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071501(11), NM_071526(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071501 NCBI Caenorhabditis elegans T28A11.20 (T28A11.20) mRNA, complete cds. 11/11 None
NM_071526 NCBI Caenorhabditis elegans C17B7.10 (C17B7.10) mRNA, complete cds. 11/11 None
SNAP00000020309 ENSEMBL cdna:SNAP chromosome:CEL140:V:3340040:3341832:-1 11/11 None
GENEFINDER00000020323 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3340040:3342237:-1 11/11 None
SNAP00000028214 ENSEMBL cdna:SNAP chromosome:CEL140:V:3271222:3273083:-1 11/11 None
GENEFINDER00000028240 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3269911:3272715:-1 11/11 None
T28A11.20 ENSEMBL cdna:known chromosome:CEL140:V:3271222:3272906:-1 gene:T28A11.20 11/11 None
C17B7.10 ENSEMBL cdna:known chromosome:CEL140:V:3340040:3342571:-1 gene:C17B7.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3271221-3272936(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl T28A11.20
C17B7.10
Entrez Gene 182710 Entrez gene
189025 Entrez gene
SwissProt O45157 EMBL-EBI
P91519 EMBL-EBI
WormBase CE16858 Wormbase
CE35790 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503902 T28A11.20 [Caenorhabditis elegans] gb|AAC48128.2| Hypothetical protein T28A11.20 [Caenorhabditis elegans] 0.0
blast AAC04404 Hypothetical protein C17B7.10 [Caenorhabditis elegans] ref|NP_503927.1| C17B7.10 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 3.8E-73
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.7E-72
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.7E-72
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.7E-72
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.1E-71
Sequence
Target Sequence 
>C. ELEGANS:186522_S_AT
tacttcattaccacacacgaaattatgcatttaatttatccagttgccaatagttatata
aacgaaagtgtatggtccggtgctctcgaatgtacccttcgtgatgttaaaatgttagga
gagactgatgcagtgaaacccgtggatggatggtataatgcagatattggctacgaagat
ctgatcaatgtaatggcgatgagagttcttatgaaaatggcagcggagaagtcagtcaat
gagcagcagctgaaagaagccctggaaactgtaatcggaggtctttgcaagcacactggt
aggaaaaacgagttgatccccgaccatcatccgttggaaatttctcgaaatgcggcagta
cgtcaatatccgttattcaactcattgtatggatgtaaggcgggagatcgtatgtttgca
aaaccaaatgagtattgcaaaccactaggggatcccatcaacgttgaggattttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTTCATTACCACACACGAAATTA 605 643 784 Antisense
GCATTTAATTTATCCAGTTGCCAAT 157 307 810 Antisense
TGCTCTCGAATGTACCCTTCGTGAT 631 579 864 Antisense
GACTGATGCAGTGAAACCCGTGGAT 490 371 906 Antisense
GAGCAGCAGCTGAAAGAAGCCCTGG 665 389 1024 Antisense
GAAGCCCTGGAAACTGTAATCGGAG 70 345 1039 Antisense
CTGTAATCGGAGGTCTTTGCAAGCA 535 239 1052 Antisense
CCCGACCATCATCCGTTGGAAATTT 153 259 1102 Antisense
GAAATGCGGCAGTACGTCAATATCC 71 363 1130 Antisense
GTCAATATCCGTTATTCAACTCATT 632 463 1145 Antisense
GATCCCATCAACGTTGAGGATTTTC 222 417 1234 Antisense
Sequence Source Affymetrix Proprietary Database