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[186517_at]

Description H04M03.12 /REP_DB=WormBase Gene ID /WP=CE20931 /TR=Q9TYQ7 /GB=AAD12797.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 59      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 72      6grp_total > 38      4grp_total > 21      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 823
1 2 3 4 range
A 437.7
(-0.6)
P 		286.4
(-1.7)
P 		114
(-3)
P 		937.2
(3)
P 		823
B 549
(1.3)
P 		269.5
(-1.9)
P 		174.8
(-3)
P 		692.4
(3)
P 		518
C 511.4
(0.9)
P 		322.9
(-1)
P 		132.7
(-3)
P 		709.7
(3)
P 		577
avr 499.4
(0.4) 		292.9
(-1.6) 		140.5
(-3) 		779.8
(3) 		639

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 532.9
(3)
P 		357.5
(-1.9)
P 		405.1
(-0.5)
P 		316.1
(-3)
P 		521.5
(2.7)
P 		402.3
(-0.6)
P 		217
B 368.7
(-2.9)
P 		487.4
(2.6)
P 		366.2
(-3)
P 		396.2
(-1.6)
P 		445.3
(0.7)
P 		495.4
(3)
P 		129
C 376.1
(-2.1)
P 		538.3
(3)
P 		393.1
(-1.6)
P 		347.2
(-3)
P 		386.8
(-1.8)
P 		473.8
(1)
P 		191
avr 425.9
(1) 		461.1
(3) 		388.1
(-1.1) 		353.2
(-3) 		451.2
(2.4) 		457.2
(2.8) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 159.4
(3)
P 		62.2
(-1.5)
P 		29.8
(-3)
P 		137.2
(2)
P 		130
B 175.1
(3)
P 		66.5
(-1.8)
P 		40.5
(-3)
P 		147.5
(1.8)
P 		135
C 169.3
(3)
P 		92.8
(-0.7)
P 		46.2
(-3)
P 		119.7
(0.6)
P 		123
avr 167.9
(3) 		73.8
(-1.4) 		38.8
(-3) 		134.8
(1.5) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 98.2
(-1.6)
P 		127.1
(0.6)
P 		88.2
(-2.4)
P 		80.2
(-3)
P 		157.4
(3)
P 		98
(-1.6)
P 		77
B 128.3
(-0.5)
P 		162.2
(1.6)
P 		88.9
(-3)
P 		104.9
(-2)
P 		180.9
(2.8)
P 		184.7
(3)
P 		96
C 139.4
(1.4)
P 		164.3
(3)
P 		100.1
(-1.1)
P 		69.8
(-3)
P 		125.2
(0.5)
P 		158.6
(2.6)
P 		95
avr 122
(0.2) 		151.2
(2.7) 		92.4
(-2.4) 		85
(-3) 		154.5
(3) 		147.1
(2.4) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186517_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10757
Sequence Type Exemplar sequence
Representative Public ID H04M03.12 NCBI
Target Description H04M03.12 /REP_DB=WormBase Gene ID /WP=CE20931 /TR=Q9TYQ7 /GB=AAD12797.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068487(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068487 NCBI Caenorhabditis elegans H04M03.12 (H04M03.12) mRNA, complete cds. 11/11 None
GENEFINDER00000034807 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5899710:5901397:-1 10/11 None
H04M03.12 ENSEMBL cdna:known chromosome:CEL140:IV:5899651:5901309:-1 gene:H04M03.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034805 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5899656-5901315(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H04M03.12
Entrez Gene 186690 Entrez gene
SwissProt Q9TYQ7 EMBL-EBI
WormBase CE20931 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD12797 Hypothetical protein H04M03.12 [Caenorhabditis elegans] ref|NP_500888.1| H04M03.12 [Caenorhabditis elegans] 1.0E-124
blast AAD12797 Hypothetical protein H04M03.12 [Caenorhabditis elegans] ref|NP_500888.1| H04M03.12 [Caenorhabditis elegans] 3.0E-92
blast CAE72628 Hypothetical protein CBG19826 [Caenorhabditis briggsae] 7.0E-77
blast CAE72628 Hypothetical protein CBG19826 [Caenorhabditis briggsae] 8.0E-62
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500888.1 1 195-217
H04M03.12 1 195-217
Sequence
Target Sequence 
>C. ELEGANS:186517_AT
ctggactcaacgcctcgtgaagaacatatgtactttttatgggtaaaggatacggaatgc
aataagataacagtaatatcaataaaaacattggaaaatcacttaaagaatgatagtcgt
gtgcggagagtaaaatggaggcatttatcatctgaaagagtttccatttatcgatatagt
ttgcctgcgatcacttatgaaatgagttttataaagtttattattcaagaaatttcgaaa
ctccaacatcagacacatttttttctgtataaacaaaatttactttttctaaaacatgcc
aagaattgcttcacatccttatttttgaaagaaacactgtatggaaaaaaatttcaaatt
catattcaaatactgtttcaaaaatccaaaatgtgtaaagaatgtatgtgccgtcacaag
aagctgtgtcttctcctcgtctggcttgcatttgtactatttctctgccttgtcgttgtt
cttggatccttatcaccagatgatagaacacatccatttgctcataatcaatcaagaaca
attccacctcaatcactgcttttagcccaacaacagccacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGACTCAACGCCTCGTGAAGAAC 471 241 172 Antisense
GATATAGTTTGCCTGCGATCACTTA 22 427 344 Antisense
GAAACTCCAACATCAGACACATTTT 391 357 408 Antisense
GAATTGCTTCACATCCTTATTTTTG 50 325 474 Antisense
ACAAGAAGCTGTGTCTTCTCCTCGT 699 113 587 Antisense
CCTCGTCTGGCTTGCATTTGTACTA 299 267 606 Antisense
GCATTTGTACTATTTCTCTGCCTTG 242 309 619 Antisense
GTTGTTCTTGGATCCTTATCACCAG 211 437 646 Antisense
GAACACATCCATTTGCTCATAATCA 73 353 677 Antisense
TCAAGAACAATTCCACCTCAATCAC 641 631 703 Antisense
TGCTTTTAGCCCAACAACAGCCACC 562 579 728 Antisense
Sequence Source Affymetrix Proprietary Database