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[186516_at]

Description T28A11.21 /REP_DB=WormBase Gene ID /WP=CE14319 /TR=P91522 /GB=AAC48129.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 52      6grp_total > 62      4grp_total > 11      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 375
1 2 3 4 range
A 451.6
(3)
P 		389.3
(-3)
P 		391.4
(-2.8)
P 		433.9
(1.3)
P 		62
B 307.3
(-3)
P 		517
(2.2)
P 		450.6
(0.6)
P 		548.4
(3)
P 		241
C 591.2
(0.5)
P 		486.7
(-2.5)
P 		468.4
(-3)
P 		681.8
(3)
P 		213
avr 450
(-2.3) 		464.3
(-1.6) 		436.8
(-3) 		554.7
(3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1009
1 2 3 4 5 6 range
A 315.4
(-3)
P 		600.9
(-1.2)
P 		1275.3
(3)
P 		798.6
(0)
P 		518
(-1.7)
P 		316.8
(-3)
P 		960
B 563.5
(-2.4)
P 		674.5
(-1.6)
P 		1317.3
(3)
P 		919.6
(0.2)
P 		474.5
(-3)
P 		524.7
(-2.6)
P 		843
C 346.6
(-2.7)
P 		830.6
(1.2)
P 		701.7
(0.1)
P 		1061.5
(3)
P 		308.2
(-3)
P 		491.5
(-1.5)
P 		753
avr 408.5
(-3) 		702
(-0.4) 		1098.1
(3) 		926.6
(1.5) 		433.6
(-2.8) 		444.3
(-2.7) 		690
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 164.4
(3)
P 		84.5
(-1.8)
P 		102.3
(-0.7)
P 		63.5
(-3)
P 		101
B 98
(-3)
P 		127.7
(3)
P 		104.4
(-1.7)
P 		116.9
(0.8)
P 		30
C 195.7
(3)
P 		139.9
(-1.1)
P 		163.2
(0.6)
P 		115
(-3)
P 		81
avr 152.7
(3) 		117.4
(-0.9) 		123.3
(-0.3) 		98.5
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 262
1 2 3 4 5 6 range
A 58.1
(-3)
P 		213.7
(1.3)
P 		277.7
(3)
P 		202.5
(0.9)
P 		156.3
(-0.3)
P 		77.1
(-2.5)
P 		220
B 196.1
(-2.8)
P 		224.5
(-1.5)
P 		319.9
(3)
P 		243.6
(-0.6)
P 		192.8
(-3)
P 		195.7
(-2.9)
P 		127
C 128.5
(-1.9)
P 		253.4
(3)
P 		178.6
(0.1)
P 		213.5
(1.4)
P 		99.7
(-3)
P 		164.5
(-0.5)
P 		154
avr 127.6
(-3) 		230.5
(1.7) 		258.7
(3) 		219.9
(1.2) 		149.6
(-2) 		145.8
(-2.2) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186516_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15623
Sequence Type Exemplar sequence
Representative Public ID T28A11.21 NCBI
Target Description T28A11.21 /REP_DB=WormBase Gene ID /WP=CE14319 /TR=P91522 /GB=AAC48129.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071504(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071504 NCBI Caenorhabditis elegans T28A11.21 (T28A11.21) mRNA, complete cds. 10/11 None
SNAP00000028217 ENSEMBL cdna:SNAP chromosome:CEL140:V:3276241:3277957:-1 10/11 None
GENEFINDER00000028232 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3276913:3277957:-1 10/11 None
T28A11.21 ENSEMBL cdna:known chromosome:CEL140:V:3276913:3277957:-1 gene:T28A11.21 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3276436-3277957(-) 87.78 100.0
Public Domain and Genome References
Gene Title
Ensembl T28A11.21
Entrez Gene 189026 Entrez gene
SwissProt P91522 EMBL-EBI
WormBase CE32215 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503905 T28A11.21 [Caenorhabditis elegans] gb|AAC48129.2| Hypothetical protein T28A11.21 [Caenorhabditis elegans] 1.0E-171
blast NP_503905 T28A11.21 [Caenorhabditis elegans] gb|AAC48129.2| Hypothetical protein T28A11.21 [Caenorhabditis elegans] 1.0E-168
blast AAC04395 Hypothetical protein C17B7.11 [Caenorhabditis elegans] ref|NP_503929.1| C17B7.11 [Caenorhabditis elegans] 1.0E-151
blast AAC04395 Hypothetical protein C17B7.11 [Caenorhabditis elegans] ref|NP_503929.1| C17B7.11 [Caenorhabditis elegans] 1.0E-149
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 2.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.8E-62
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 8.0E-11
Sequence
Target Sequence 
>C. ELEGANS:186516_AT
gtcttgatgatcaatccctggaagcaattaaattgagcaatccaagccaaattaatcaac
tcgcccagattactcatttgaatcaatggaaaactgcaaagaattttgaactttgcaatg
gaaaactggacaacagtgaacaaattgagcatctcttccactttgaggaatttggttgcg
gaactgatgttttctcagtatcaaatgcagttaaagttagagatgatcttatgaaaagga
gttcatttcaaacatgccgcatttatttcaatgtgtcacagtcaaatccagtcgatattg
caaaagttttcaagccggactatgttggtggcaatgaatattcgtttgaatattcggatg
atatccacaaatttgctattgattgtttccgcgttggtgaatctcatcggttttctgatg
cgtacaatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTTGATGATCAATCCCTGGAAGC 113 469 524 Antisense
ACAAATTGAGCATCTCTTCCACTTT 594 111 663 Antisense
GAGCATCTCTTCCACTTTGAGGAAT 405 389 670 Antisense
TTGGTTGCGGAACTGATGTTTTCTC 88 709 695 Antisense
AGGAGTTCATTTCAAACATGCCGCA 187 57 760 Antisense
GCCGCATTTATTTCAATGTGTCACA 583 283 779 Antisense
CGGATGATATCCACAAATTTGCTAT 286 243 878 Antisense
AATTTGCTATTGATTGTTTCCGCGT 188 175 893 Antisense
TGTTTCCGCGTTGGTGAATCTCATC 269 561 907 Antisense
GCGTTGGTGAATCTCATCGGTTTTC 450 291 914 Antisense
TCGGTTTTCTGATGCGTACAATTTT 500 605 930 Antisense
Sequence Source Affymetrix Proprietary Database