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[186505_at]

Description F49H12.3 /REP_DB=WormBase Gene ID /WP=CE20837 /TR=Q9N5S5 /GB=AAF39829.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 22      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 103.1
(2.9)
P 		47.7
(-1.3)
A 		25.8
(-3)
A 		104.5
(3)
A 		79
B 147.4
(3)
P 		63.8
(-3)
A 		64.1
(-3)
P 		130.4
(1.8)
P 		84
C 122.4
(2.4)
P 		94.1
(0.8)
A 		27.4
(-3)
P 		133.2
(3)
P 		106
avr 124.3
(3) 		68.5
(-0.9) 		39.1
(-3) 		122.7
(2.9) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 44.7
(-0.4)
A 		39.5
(-0.9)
A 		81.7
(3)
P 		48.9
(-0)
P 		16.7
(-3)
A 		34.6
(-1.3)
A 		65
B 88.6
(3)
P 		78.2
(1.9)
P 		33.6
(-3)
A 		35.5
(-2.8)
A 		48.1
(-1.4)
A 		55.5
(-0.6)
M 		55
C 59.1
(-0.7)
P 		71.8
(2)
P 		65
(0.6)
A 		76.8
(3)
A 		50.6
(-2.4)
A 		47.8
(-3)
A 		29
avr 64.1
(3) 		63.2
(2.8) 		60.1
(2.1) 		53.7
(0.6) 		38.5
(-3) 		46
(-1.2) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 37.5
(3)
P 		10.4
(-2.3)
A 		6.7
(-3)
A 		15.3
(-1.3)
A 		31
B 47
(3)
P 		15.8
(-2.8)
A 		14.9
(-3)
P 		27.8
(-0.6)
P 		32
C 40.5
(3)
P 		27
(0.4)
A 		9.6
(-3)
P 		22.5
(-0.5)
P 		31
avr 41.7
(3) 		17.7
(-1.6) 		10.4
(-3) 		21.9
(-0.8) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 8.2
(-1.5)
A 		14.1
(1.3)
A 		17.8
(3)
P 		12.4
(0.5)
P 		5
(-3)
A 		8.4
(-1.4)
A 		13
B 30.8
(3)
P 		26
(1.7)
P 		8.2
(-3)
A 		9.4
(-2.7)
A 		19.5
(0)
A 		20.7
(0.3)
M 		23
C 21.9
(3)
P 		21.9
(3)
P 		16.6
(-1.9)
A 		15.4
(-3)
A 		16.4
(-2.1)
A 		16
(-2.4)
A 		7
avr 20.3
(2.7) 		20.7
(3) 		14.2
(-1.7) 		12.4
(-3) 		13.6
(-2.1) 		15
(-1.1) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186505_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18251
Sequence Type Exemplar sequence
Representative Public ID F49H12.3 NCBI
Target Description F49H12.3 /REP_DB=WormBase Gene ID /WP=CE20837 /TR=Q9N5S5 /GB=AAF39829.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075982(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075982 NCBI Caenorhabditis elegans F49H12.3 (F49H12.3) mRNA, complete cds. 11/11 None
SNAP00000015651 ENSEMBL cdna:SNAP chromosome:CEL140:X:2084370:2084990:1 11/11 None
GENEFINDER00000015662 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2084370:2084990:1 11/11 None
F49H12.3 ENSEMBL cdna:known chromosome:CEL140:X:2084370:2084990:1 gene:F49H12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2084369-2084990(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49H12.3
Entrez Gene 180521 Entrez gene
SwissProt Q9N5S5 EMBL-EBI
WormBase CE20837 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39829 Hypothetical protein F49H12.3 [Caenorhabditis elegans] ref|NP_508383.1| F49H12.3 [Caenorhabditis elegans] 4.0E-75
blast CAE68358 Hypothetical protein CBG14096 [Caenorhabditis briggsae] 1.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 9.1E-9
Sequence
Target Sequence 
>C. ELEGANS:186505_AT
ggaggaactccaatggcaaccttgaagacgactttcccagttgactcaattttccacaaa
tggtttgtgtcaagaacaaaggcgtccccattcacaagcgaccgagatggcgcgtacttt
gtcgatagagacccattttcattcggcgtcgtgctcaactattttcgtctccggaaagct
ggacagctttgggaagcctgtctaccaaaagacccagacaggttggcaatgctgacacag
gaagcagatttcttccttctgcctcaactacgtgatcaagcaatctgcatgcttcagcta
tgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGGAACTCCAATGGCAACCTTGA 601 515 46 Antisense
GGCAACCTTGAAGACGACTTTCCCA 20 535 60 Antisense
ACGACTTTCCCAGTTGACTCAATTT 229 91 73 Antisense
GTGTCAAGAACAAAGGCGTCCCCAT 551 483 112 Antisense
GAGATGGCGCGTACTTTGTCGATAG 126 397 149 Antisense
TAGAGACCCATTTTCATTCGGCGTC 238 651 171 Antisense
TTCGTCTCCGGAAAGCTGGACAGCT 63 695 209 Antisense
ACAGCTTTGGGAAGCCTGTCTACCA 239 109 228 Antisense
GGAAGCAGATTTCTTCCTTCTGCCT 458 531 285 Antisense
CTTCTGCCTCAACTACGTGATCAAG 145 221 301 Antisense
GCAATCTGCATGCTTCAGCTATGTA 305 321 325 Antisense
Sequence Source Affymetrix Proprietary Database