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[186499_at]

Description ZK1248.1 /REP_DB=WormBase Gene ID /WP=CE19646 /TR=Q23427 /GB=AAC71099.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 70      4grp_total > 34      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 28.8
(-1)
A 		52.5
(3)
A 		17.2
(-3)
A 		52.6
(3)
A 		35
B 4
(-3)
A 		71
(3)
P 		39.4
(0.2)
P 		31.7
(-0.5)
A 		67
C 20.8
(-3)
A 		40
(2.4)
P 		32.3
(0.2)
P 		42.1
(3)
A 		21
avr 17.9
(-3) 		54.5
(3) 		29.6
(-1.1) 		42.1
(1) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 28
(-0.2)
A 		18
(-3)
A 		28
(-0.2)
A 		27.6
(-0.3)
P 		39.5
(3)
A 		32
(0.9)
A 		22
B 11.3
(-3)
A 		22.8
(-1.3)
A 		52.9
(3)
A 		40.9
(1.3)
A 		28.1
(-0.6)
A 		25.2
(-1)
A 		42
C 24.6
(-1.8)
A 		11.1
(-3)
A 		40.2
(-0.5)
A 		80.1
(3)
A 		19.5
(-2.3)
A 		27.8
(-1.5)
A 		69
avr 21.3
(-2.3) 		17.3
(-3) 		40.4
(1.3) 		49.5
(3) 		29
(-0.8) 		28.3
(-1) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 10.5
(2.2)
A 		11.4
(3)
A 		4.5
(-3)
A 		7.7
(-0.2)
A 		7
B 1.3
(-3)
A 		17.5
(3)
P 		9.1
(-0.1)
P 		6.8
(-1)
A 		16
C 6.9
(-3)
A 		11.5
(3)
P 		11.2
(2.6)
P 		7.1
(-2.7)
A 		5
avr 6.2
(-3) 		13.5
(3) 		8.3
(-1.3) 		7.2
(-2.2) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 5.2
(-3)
A 		6.4
(-1.9)
A 		6.1
(-2.2)
A 		7
(-1.4)
P 		11.9
(3)
A 		7.8
(-0.7)
A 		7
B 3.9
(-3)
A 		7.6
(-0.5)
A 		12.9
(3)
A 		10.8
(1.6)
A 		11.4
(2)
A 		9.4
(0.7)
A 		9
C 9.1
(-0.3)
A 		3.4
(-3)
A 		10.2
(0.2)
A 		16.1
(3)
A 		6.3
(-1.6)
A 		9.3
(-0.2)
A 		13
avr 6.1
(-2.7) 		5.8
(-3) 		9.7
(1.3) 		11.3
(3) 		9.9
(1.5) 		8.8
(0.3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186499_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4232
Sequence Type Exemplar sequence
Representative Public ID ZK1248.1 NCBI
Target Description ZK1248.1 /REP_DB=WormBase Gene ID /WP=CE19646 /TR=Q23427 /GB=AAC71099.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062763(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062763 NCBI Caenorhabditis elegans ZK1248.1 (ZK1248.1) mRNA, complete cds. 11/11 None
SNAP00000041383 ENSEMBL cdna:SNAP chromosome:CEL140:II:5841902:5844540:1 11/11 None
GENEFINDER00000041387 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5842208:5844540:1 11/11 None
ZK1248.1 ENSEMBL cdna:known chromosome:CEL140:II:5842208:5844540:1 gene:ZK1248.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5842204-5844537(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK1248.1
Entrez Gene 191543 Entrez gene
SwissProt Q23427 EMBL-EBI
WormBase CE35373 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC71099 Hypothetical protein ZK1248.1 [Caenorhabditis elegans] ref|NP_495164.2| ZK1248.1 [Caenorhabditis elegans] 0.0
blast CAE59207 Hypothetical protein CBG02520 [Caenorhabditis briggsae] 1.0E-114
blast CAE59207 Hypothetical protein CBG02520 [Caenorhabditis briggsae] 1.0E-105
blast CAE69323 Hypothetical protein CBG15395 [Caenorhabditis briggsae] 7.0E-90
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 5.8E-84
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 5.8E-84
Sequence
Target Sequence 
>C. ELEGANS:186499_AT
aaatgagttcctgcttcttctgaatacggactacttttctgatttgtacgggagcatcgg
gttttcaatgatccatgaaatcatgcacacattggtatttgacgataacgatctagacaa
gccactttcaaaactttggacaaaaaatgcagattgtgtaaaggatcaagcactcaaaac
atgtgaaacgtttccgactgtaactgaaatgcataacatgaaaccagcgtgtaatactac
agtaacattcgaagaagatgctgctgatttagctgcgtatcgaattgcgtggagaattta
cgaaaactcctatgccagaaaaacggttgttccgaactatgaggcccttgataaaaagca
attattcttctacggagctgcagttttcttttgcaatcaggatggaatgtccagagttcc
atttcaaaaccttgcacattcaaacaactaccagagagtcaattccttgatgtctcaaat
gaaacagttctccgatgcatttaagtgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGAGTTCCTGCTTCTTCTGAAT 657 119 879 Antisense
GAATACGGACTACTTTTCTGATTTG 17 325 900 Antisense
TGTACGGGAGCATCGGGTTTTCAAT 446 559 923 Antisense
TAACGATCTAGACAAGCCACTTTCA 684 631 984 Antisense
GTGAAACGTTTCCGACTGTAACTGA 657 459 1061 Antisense
GATTTAGCTGCGTATCGAATTGCGT 405 429 1144 Antisense
ATTTACGAAAACTCCTATGCCAGAA 694 1 1174 Antisense
TTGTTCCGAACTATGAGGCCCTTGA 29 675 1205 Antisense
CAATTATTCTTCTACGGAGCTGCAG 64 179 1237 Antisense
GGAGCTGCAGTTTTCTTTTGCAATC 514 521 1252 Antisense
CAGTTCTCCGATGCATTTAAGTGTA 614 205 1363 Antisense
Sequence Source Affymetrix Proprietary Database