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[186497_at]

Description Y58G8A.4 /REP_DB=WormBase Gene ID /WP=CE24481 /TR=Q9N4R4 /GB=AAF60816.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 03      4grp_high_8_8 > 20      6grp_total > 15      4grp_total > 21      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 83.2
(-0.5)
P 		76.9
(-1.4)
P 		64.6
(-3)
P 		109.7
(3)
P 		45
B 74.9
(3)
P 		74.8
(3)
P 		40.2
(-3)
P 		66.5
(1.5)
P 		35
C 64.7
(-0.5)
P 		46.6
(-2.8)
A 		45.1
(-3)
P 		92.3
(3)
P 		47
avr 74.3
(0.7) 		66.1
(-0.6) 		50
(-3) 		89.5
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 75.5
(3)
P 		57.3
(0.8)
P 		34.5
(-1.9)
A 		31.9
(-2.3)
P 		44.6
(-0.7)
M 		25.8
(-3)
P 		50
B 62
(0.6)
P 		81.2
(3)
P 		61.5
(0.5)
P 		57.7
(0.1)
P 		36.1
(-2.6)
P 		33.2
(-3)
A 		48
C 88.5
(3)
P 		48.4
(-1.6)
M 		68.5
(0.7)
A 		38
(-2.8)
A 		36
(-3)
P 		56.7
(-0.6)
P 		53
avr 75.3
(3) 		62.3
(0.9) 		54.8
(-0.4) 		42.5
(-2.4) 		38.9
(-3) 		38.6
(-3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 30.3
(3)
P 		16.7
(-2.7)
P 		16.9
(-2.7)
P 		16.1
(-3)
P 		14
B 23.9
(3)
P 		18.5
(0.8)
P 		9.3
(-3)
P 		14.2
(-1)
P 		15
C 21.4
(3)
P 		13.4
(-3)
A 		15.7
(-1.3)
P 		15.6
(-1.4)
P 		8
avr 25.2
(3) 		16.2
(-1.8) 		14
(-3) 		15.3
(-2.3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 13.9
(0.2)
P 		20.4
(3)
P 		7.5
(-2.5)
A 		8.1
(-2.2)
P 		13.5
(0.1)
M 		6.3
(-3)
P 		14
B 21.6
(0.8)
P 		27
(3)
P 		14.9
(-2)
P 		15.3
(-1.8)
P 		14.7
(-2.1)
P 		12.4
(-3)
A 		15
C 32.8
(3)
P 		14.8
(-1.3)
M 		17.4
(-0.7)
A 		7.6
(-3)
A 		11.7
(-2)
P 		19
(-0.3)
P 		25
avr 22.8
(3) 		20.7
(2) 		13.3
(-1.6) 		10.3
(-3) 		13.3
(-1.6) 		12.6
(-1.9) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186497_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15915
Sequence Type Exemplar sequence
Representative Public ID Y58G8A.4 NCBI
Target Description Y58G8A.4 /REP_DB=WormBase Gene ID /WP=CE24481 /TR=Q9N4R4 /GB=AAF60816.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029094, NM_001029093
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
Y58G8A.4a ENSEMBL cdna:known chromosome:CEL140:V:276819:281545:-1 gene:Y58G8A.4 A 
NM_001029093 NCBI Caenorhabditis elegans Y58G8A.4a (Y58G8A.4) mRNA, complete cds. None
Y58G8A.4b ENSEMBL cdna:known chromosome:CEL140:V:276840:281533:-1 gene:Y58G8A.4 A 
SNAP00000024889 ENSEMBL cdna:SNAP chromosome:CEL140:V:277954:281533:-1 None
NM_001029094 NCBI Caenorhabditis elegans Y58G8A.4b (Y58G8A.4) mRNA, complete cds. None
GENEFINDER00000024891 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:280393:281545:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:281220-282054(-) 98.88 100.0
Public Domain and Genome References
Ensembl Y58G8A.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60815 Hypothetical protein Y58G8A.4a [Caenorhabditis elegans] ref|NP_001024264.1| Y58G8A.4a [Caenorhabditis elegans] 0.0
blast AAT68889 Hypothetical protein Y58G8A.4b [Caenorhabditis elegans] ref|NP_001024265.1| Y58G8A.4b [Caenorhabditis elegans] 0.0
blast CAE58004 Hypothetical protein CBG01072 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.9E-49
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.1E-44
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.5E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024264.1 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
NP_001024265.1 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
Y58G8A.4a 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
Y58G8A.4b 7 57-79,92-114,129-151,170-192,226-248,286-308,323-345
SNAP00000024889 5 57-79,92-114,129-151,170-192,226-248
GENEFINDER00000024891 2 61-83,96-118
Sequence
Target Sequence 
>C. ELEGANS:186497_AT
gttttttgaactacctggcacattttaaagcgggtttcgagattgatttatttaacttca
ttaacataacaatttataagaaatttccttttttttttcatcgactgaaatttgatagaa
aattggattttatgattaatttccagaacacgcttttcgattttcccattttagaaaaaa
acatttttcgattttcgaatttaaaaaatttaaaaactctaaaattttcaacgatatttt
tattttatggattttatgcatgtaattttcttcaaaacgtttttataaccttcgatgcat
ggttagttcggcggccaccatttcgaccatttcaaccacaacgactccctccaccatcag
caacgttatcacaagtcattcgaacaatggctcgtgcattcagatcgctgaggcgattgc
ggcacaaggcatcgatgatattactgtgtaagttttgggtatcctggatggattttctaa
cttataatttccaaaaaaacctgtcaacttttctcaccacaaaaatcacaatttccgtca
tagttcaacgatctcatctttttcaccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTTTGAACTACCTGGCACATTT 238 449 29 Antisense
TCCAGAACACGCTTTTCGATTTTCC 147 591 170 Antisense
CGATGCATGGTTAGTTCGGCGGCCA 88 249 321 Antisense
CATTTCGACCATTTCAACCACAACG 235 215 347 Antisense
ACCATCAGCAACGTTATCACAAGTC 40 87 381 Antisense
AAGTCATTCGAACAATGGCTCGTGC 254 157 401 Antisense
TGGCTCGTGCATTCAGATCGCTGAG 278 545 416 Antisense
GCTGAGGCGATTGCGGCACAAGGCA 432 295 435 Antisense
CTGTCAACTTTTCTCACCACAAAAA 162 229 529 Antisense
AAATCACAATTTCCGTCATAGTTCA 448 117 551 Antisense
TTCAACGATCTCATCTTTTTCACCC 514 685 572 Antisense
Sequence Source Affymetrix Proprietary Database