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[186483_at]

Description Y54G11A.11 /REP_DB=WormBase Gene ID /WP=CE22483 /TR=Q9XVZ8 /GB=CAA22454.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 86      6grp_total > 49      4grp_total > 12      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 676
1 2 3 4 range
A 778.6
(-2.7)
P 		845.5
(-2.1)
P 		742.4
(-3)
P 		1407.9
(3)
P 		666
B 929
(-0.6)
P 		824.2
(-2)
P 		750.9
(-3)
P 		1204.4
(3)
P 		454
C 1102.4
(2.1)
P 		939
(-0.1)
P 		731.6
(-3)
P 		1165.3
(3)
P 		434
avr 936.7
(-0.7) 		869.6
(-1.5) 		741.6
(-3) 		1259.2
(3) 		518

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 642
1 2 3 4 5 6 range
A 1271.7
(3)
P 		673.8
(-3)
P 		720.6
(-2.5)
P 		826
(-1.5)
P 		1078.3
(1.1)
P 		1121.1
(1.5)
P 		598
B 1131.5
(1.1)
P 		846.7
(-2)
P 		798
(-2.6)
P 		760
(-3)
P 		1298.8
(3)
P 		1266.2
(2.6)
P 		539
C 1100
(1.9)
P 		991.1
(0.7)
P 		656.6
(-3)
P 		910.2
(-0.2)
P 		854.4
(-0.8)
P 		1204
(3)
P 		547
avr 1167.7
(2.6) 		837.2
(-1.6) 		725.1
(-3) 		832.1
(-1.6) 		1077.2
(1.5) 		1197.1
(3) 		472
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 283.5
(3)
P 		183.5
(-3)
P 		194.1
(-2.4)
P 		206.1
(-1.6)
P 		100
B 296.2
(3)
P 		203.5
(-1.6)
P 		174
(-3)
P 		256.7
(1.1)
P 		122
C 364.9
(3)
P 		270
(-0.4)
P 		255
(-0.9)
P 		196.5
(-3)
P 		168
avr 314.9
(3) 		219
(-2.4) 		207.7
(-3) 		219.8
(-2.3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 371
1 2 3 4 5 6 range
A 234.4
(-0.2)
P 		239.6
(-0.1)
P 		156.9
(-3)
P 		209.5
(-1.1)
P 		325.4
(3)
P 		273
(1.1)
P 		169
B 393.7
(0.6)
P 		281.8
(-1.4)
P 		193.8
(-3)
P 		201.3
(-2.9)
P 		527.7
(3)
P 		472.1
(2)
P 		334
C 407.7
(3)
P 		302.4
(0.4)
P 		167.1
(-3)
P 		183
(-2.6)
P 		276.5
(-0.3)
P 		403
(2.9)
P 		241
avr 345.3
(1.9) 		274.6
(-0.1) 		172.6
(-3) 		197.9
(-2.3) 		376.5
(2.8) 		382.7
(3) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186483_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3968
Sequence Type Exemplar sequence
Representative Public ID Y54G11A.11 NCBI
Target Description Y54G11A.11 /REP_DB=WormBase Gene ID /WP=CE22483 /TR=Q9XVZ8 /GB=CAA22454.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064582(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064582 NCBI Caenorhabditis elegans Y54G11A.11 (Y54G11A.11) mRNA, complete cds. 11/11 None
SNAP00000023656 ENSEMBL cdna:SNAP chromosome:CEL140:II:14340386:14350370:1 11/11 None
GENEFINDER00000023686 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14350067:14350370:1 11/11 None
Y54G11A.11 ENSEMBL cdna:known chromosome:CEL140:II:14350041:14350697:1 gene:Y54G11A.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14350063-14350367(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54G11A.11
Entrez Gene 175090 Entrez gene
SwissProt Q9XVZ8 EMBL-EBI
WormBase CE22483 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248884_AT expressed protein at
HU35KSUBA:RC_AA455267_AT elongation factor 1 homolog (ELF1, S. cerevisiae) hs
U133_X3P:HS.326422.0.S1_3P_X_AT elongation factor 1 homolog (ELF1, S. cerevisiae) hs
U133_X3P:HS.326422.0.S1_3P_AT elongation factor 1 homolog (ELF1, S. cerevisiae) hs
HG-U133_PLUS_2:225156_AT elongation factor 1 homolog (ELF1, S. cerevisiae) hs
HG-U133B:225156_AT elongation factor 1 homolog (ELF1, S. cerevisiae) hs
HG-U95B:51139_AT elongation factor 1 homolog (ELF1, S. cerevisiae) hs
MG-U74AV2:95760_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MG-U74BV2:164409_I_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MG-U74CV2:170864_I_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MG-U74CV2:170333_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MOE430A:1423820_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MOUSE430A_2:1423820_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MOUSE430_2:1423820_AT elongation factor 1 homolog (ELF1, S. cerevisiae) mm
MU19KSUBB:TC34443_I_AT Elongation factor 1 homolog (ELF1, S. cerevisiae), mRNA (cDNA clone MGC:37411 IMAGE:4981209) mm
MU19KSUBB:TC34443_R_AT Elongation factor 1 homolog (ELF1, S. cerevisiae), mRNA (cDNA clone MGC:37411 IMAGE:4981209) mm
MU19KSUBB:TC34443_S_AT Elongation factor 1 homolog (ELF1, S. cerevisiae), mRNA (cDNA clone MGC:37411 IMAGE:4981209) mm
MU19KSUBC:TC36895_AT Elongation factor 1 homolog (ELF1, S. cerevisiae), mRNA (cDNA clone MGC:37411 IMAGE:4981209) mm
RAE230A:1371515_AT Similar to RIKEN cDNA 1110011K10 (predicted) rn
RAT230_2:1371515_AT Similar to RIKEN cDNA 1110011K10 (predicted) rn
YEAST_2:1779859_AT Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Sc
Protein Similarities
Method ID Description E-Value
blast CAA22454 Hypothetical protein Y54G11A.11 [Caenorhabditis elegans] ref|NP_496983.1| Y54G11A.11 [Caenorhabditis elegans] sp|Q9XVZ8|U222_CAEEL Hypothetical UPF0222 protein Y54G11A.11 in chromosome II 2.0E-45
blast CAE65930 Hypothetical protein CBG11103 [Caenorhabditis briggsae] 1.0E-44
blast AAM76200 RE67573p [Drosophila melanogaster] gb|EAA46293.1| CG40228-PA.3 [Drosophila melanogaster] ref|NP_001015130.1| CG40228-PA.3 [Drosophila melanogaster] sp|Q8MQI6|U222_DROME Hypothetical UPF0222 protein CG40228 6.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR007808 EMBL-EBI Putative zinc binding domain DUF701 8.7E-40
Pfam IPR007808 EMBL-EBI Putative zinc binding domain DUF701 8.7E-40
Sequence
Target Sequence 
>C. ELEGANS:186483_AT
gaaaggctccaaccaaggcaaaggctgtaatgccactcgacacccaattcaactgtccgt
tttgcaatcatgaacgcgtttgtgaagtgaaaatggatcgcgaaaagaatgtcggttata
tctcgtgtcgtgtctgctctgaagacttccaaacaaacatcaactacctctccgagccaa
tcgacgtctattctgattgggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGGCTCCAACCAAGGCAAAGGC 300 359 35 Antisense
GGCAAAGGCTGTAATGCCACTCGAC 340 535 51 Antisense
TCGACACCCAATTCAACTGTCCGTT 67 599 71 Antisense
TCAACTGTCCGTTTTGCAATCATGA 660 629 83 Antisense
GCAATCATGAACGCGTTTGTGAAGT 110 323 98 Antisense
GAATGTCGGTTATATCTCGTGTCGT 173 333 141 Antisense
CTCGTGTCGTGTCTGCTCTGAAGAC 139 233 156 Antisense
GTGTCTGCTCTGAAGACTTCCAAAC 284 487 164 Antisense
TCCAAACAAACATCAACTACCTCTC 514 585 182 Antisense
TCCGAGCCAATCGACGTCTATTCTG 225 595 205 Antisense
AATCGACGTCTATTCTGATTGGGTG 215 169 213 Antisense
Sequence Source Affymetrix Proprietary Database