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[186471_at]

Description K02G10.6 /REP_DB=WormBase Gene ID /WP=CE04706 /TR=Q21158 /GB=AAK39252.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 22      4grp_high_8_8 > 29      6grp_total > 23      4grp_total > 34      6grp_cgc > 71      6grp_high1_sub > De      6grp_cgc_sub > 03      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 182.9
(-0.6)
P 		213.8
(3)
P 		183.4
(-0.6)
P 		162.6
(-3)
A 		51
B 119.5
(-3)
P 		206.4
(3)
P 		169.1
(0.4)
P 		175.6
(0.9)
P 		87
C 166.6
(-3)
P 		179.6
(-1)
P 		167.7
(-2.8)
P 		205.8
(3)
P 		39
avr 156.3
(-3) 		199.9
(3) 		173.4
(-0.6) 		181.3
(0.4) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 243
(3)
P 		130.9
(-1.9)
P 		229.9
(2.4)
P 		104.7
(-3)
P 		197.5
(1)
P 		121.2
(-2.3)
P 		138
B 264.4
(3)
P 		254.7
(2.4)
P 		254.6
(2.4)
P 		204.1
(-0.8)
P 		184.1
(-2.1)
P 		170
(-3)
P 		94
C 134.1
(-1.4)
P 		256.3
(3)
P 		155.9
(-0.6)
P 		213.2
(1.4)
P 		90
(-3)
M 		171.6
(-0.1)
P 		166
avr 213.8
(3) 		214
(3) 		213.5
(2.9) 		174
(-1) 		157.2
(-2.7) 		154.3
(-3) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 66.6
(3)
P 		46.4
(0.2)
P 		47.9
(0.4)
P 		23.8
(-3)
A 		43
B 38.1
(-2.7)
P 		51
(3)
P 		39.2
(-2.2)
P 		37.4
(-3)
P 		14
C 55.2
(2.2)
P 		51.6
(1.3)
P 		58.5
(3)
P 		34.7
(-3)
P 		24
avr 53.3
(3) 		49.7
(2) 		48.5
(1.6) 		32
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 44.8
(0.3)
P 		46.6
(0.6)
P 		50.1
(1.3)
P 		26.6
(-3)
P 		59.6
(3)
P 		29.5
(-2.5)
P 		33
B 92
(3)
P 		84.8
(1.9)
P 		61.8
(-1.8)
P 		54
(-3)
P 		74.8
(0.3)
P 		63.4
(-1.5)
P 		38
C 49.7
(-0.5)
P 		78.2
(3)
P 		39.7
(-1.7)
P 		42.9
(-1.3)
P 		29.1
(-3)
M 		57.4
(0.5)
P 		49
avr 62.2
(1.4) 		69.9
(3) 		50.5
(-1.1) 		41.2
(-3) 		54.5
(-0.2) 		50.1
(-1.1) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186471_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16156
Sequence Type Exemplar sequence
Representative Public ID K02G10.6 NCBI
Target Description K02G10.6 /REP_DB=WormBase Gene ID /WP=CE04706 /TR=Q21158 /GB=AAK39252.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029584(10), NM_001029585(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029584 NCBI Caenorhabditis elegans Homolog of Yeast Longevity gene family member (hyl-2) (hyl-2) mRNA, complete cds. 10/11 None
NM_001029585 NCBI Caenorhabditis elegans Homolog of Yeast Longevity gene family member (hyl-2) (hyl-2) mRNA, complete cds. 11/11 None
SNAP00000043395 ENSEMBL cdna:SNAP chromosome:CEL140:X:4697216:4699429:-1 11/11 None
GENEFINDER00000043405 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4697216:4699429:-1 11/11 None
K02G10.6b ENSEMBL cdna:known chromosome:CEL140:X:4696755:4699431:-1 gene:K02G10.6 10/11 None
K02G10.6a ENSEMBL cdna:known chromosome:CEL140:X:4697216:4699891:-1 gene:K02G10.6 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4697216-4699892(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hyl-2
Ensembl K02G10.6
Entrez Gene 180743 Entrez gene
SwissProt Q7Z139 EMBL-EBI
WormBase CE34329 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.28276.1.S1_AT similar to LAG1 longevity assurance homolog 4 cfa
CHICKEN:GGAAFFX.193.1.S1_AT similar to LAG1 longevity assurance homolog 4 gga
CHICKEN:GGAAFFX.193.2.S1_AT similar to LAG1 longevity assurance homolog 4 gga
CHICKEN:GGAAFFX.193.2.S1_S_AT similar to LAG1 longevity assurance homolog 4 gga
HG-U133_PLUS_2:218922_S_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
HG-U133A:218922_S_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
HG-U133A_2:218922_S_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
HU35KSUBB:RC_H38246_S_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
U133_X3P:G13375716_3P_X_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
U133_X3P:G13375716_3P_A_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
U133_X3P:218922_3P_S_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
HG-U95B:52955_AT LAG1 longevity assurance homolog 4 (S. cerevisiae) hs
MG-U74BV2:105180_AT longevity assurance homolog 4 (S. cerevisiae) mm
MG-U74BV2:114997_AT longevity assurance homolog 4 (S. cerevisiae) mm
MG-U74BV2:163912_AT longevity assurance homolog 4 (S. cerevisiae) mm
MG-U74CV2:167629_R_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOE430A:1417780_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOUSE430A_2:1417780_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOUSE430_2:1417780_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOE430A:1417781_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOUSE430A_2:1417781_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOUSE430_2:1417781_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOE430A:1417782_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOUSE430A_2:1417782_AT longevity assurance homolog 4 (S. cerevisiae) mm
MOUSE430_2:1417782_AT longevity assurance homolog 4 (S. cerevisiae) mm
MU19KSUBB:TC34642_AT Longevity assurance homolog 4 (S. cerevisiae), mRNA (cDNA clone MGC:7360 IMAGE:3487343) mm
RG-U34C:RC_AI136564_AT longevity assurance homolog 4 (S. cerevisiae) (predicted) rn
RAE230A:1389741_AT longevity assurance homolog 4 (S. cerevisiae) (predicted) rn
RAT230_2:1389741_AT longevity assurance homolog 4 (S. cerevisiae) (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_001024755 Homolog of Yeast Longevity gene family member (hyl-2) [Caenorhabditis elegans] 0.0
blast AAP68933 Homolog of yeast longevity gene protein 2 [Caenorhabditis elegans] ref|NP_001024756.1| Homolog of Yeast Longevity gene family member (hyl-2) [Caenorhabditis elegans] 0.0
blast T16539 hypothetical protein K02G10.6 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005547 EMBL-EBI Longevity-assurance protein (LAG1) 4.4E-109
Pfam IPR005547 EMBL-EBI Longevity-assurance protein (LAG1) 4.4E-109
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024755.1 4 176-195,218-240,304-326,353-375
NP_001024756.1 5 38-60,91-110,133-155,219-241,268-290
K02G10.6b 5 38-60,91-110,133-155,219-241,268-290
K02G10.6a 4 176-195,218-240,304-326,353-375
SNAP00000043395 5 38-60,91-110,133-155,219-241,268-290
GENEFINDER00000043405 5 38-60,91-110,133-155,219-241,268-290
Sequence
Target Sequence 
>C. ELEGANS:186471_AT
gtctgcggaattcttttcctcgatgcaaagcgcagtgatttctggcaaatgcttgttcat
cattttatcaccctcgcacttattggtgtttcatggacgatgaatatggttcgtgttgga
acgctgattcttgtcagtcacgacgcagttgatattttgattgatgtcggaaagattctt
cgatacgaacaatttgaaactgccttgacgatttgcttcgccggagttctttttgtatgg
gtggccacgaggcttgtctattatccattctggatcatcagatccgtttggtttgatgct
ccagctcttatccaagacgactacgaatggttgaactttgaccagcaacctcaagcccca
agattcatcatgcttctgttaacggcacttctcattcttcacatcttctgggcttacatt
ctgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTGCGGAATTCTTTTCCTCGATG 208 471 574 Antisense
CTTGTTCATCATTTTATCACCCTCG 120 219 625 Antisense
ACCCTCGCACTTATTGGTGTTTCAT 523 89 643 Antisense
GATTCTTGTCAGTCACGACGCAGTT 615 429 699 Antisense
AAACTGCCTTGACGATTTGCTTCGC 565 127 770 Antisense
CTTCGCCGGAGTTCTTTTTGTATGG 681 219 789 Antisense
TGGCCACGAGGCTTGTCTATTATCC 145 545 815 Antisense
TTGATGCTCCAGCTCTTATCCAAGA 303 705 866 Antisense
AGCCCCAAGATTCATCATGCTTCTG 399 83 927 Antisense
CATGCTTCTGTTAACGGCACTTCTC 453 209 942 Antisense
ACATCTTCTGGGCTTACATTCTGTT 699 105 974 Antisense
Sequence Source Affymetrix Proprietary Database